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Porous silicon nanoparticles for cancer photothermotherapy.

Hong C, Lee J, Zheng H, Hong SS, Lee C - Nanoscale Res Lett (2011)

Bottom Line: Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis.Tumors have not recurred at all in the PSi/NIR treatment groups thereafter.Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Materials Science and Engineering, Inha University, 253 Yonghyeon-dong, Incheon, 402-751, Republic of Korea. cmlee@inha.ac.kr.

ABSTRACT
The in vitro cell tests and in vivo animal tests were performed to investigate the feasibility of the photothermal therapy based on porous silicon (PSi) in combination with near-infrared (NIR) laser. According to the Annexin V- fluorescein isothiocyanate Apoptosis assay test results, the untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%. Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis. The in vivo animal test results showed that the Murine colon carcinoma (CT-26) tumors were completely resorbed without nearly giving damage to surrounding healthy tissue within 5 days of PSi and NIR laser treatment. Tumors have not recurred at all in the PSi/NIR treatment groups thereafter. Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

No MeSH data available.


Related in: MedlinePlus

Trypan blue staining. Optical microscopic images of the CT-26 cells treated with a PSi/EtOH:PEG solution (0.7 g/L) followed by NIR laser treatments (for 20 min at 600 mW/cm2) 4 times for 2 min each time with a time interval of 2 min. The cells were stained using trypan blue dye after the NIR laser treatments to examine cell damage. The cells indicated by arrows are some examples of dead cells turned blue after staining.
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Figure 4: Trypan blue staining. Optical microscopic images of the CT-26 cells treated with a PSi/EtOH:PEG solution (0.7 g/L) followed by NIR laser treatments (for 20 min at 600 mW/cm2) 4 times for 2 min each time with a time interval of 2 min. The cells were stained using trypan blue dye after the NIR laser treatments to examine cell damage. The cells indicated by arrows are some examples of dead cells turned blue after staining.

Mentions: To determine whether the effect of PSi nanoparticles under the NIR laser irradiation on cells was cytotoxic, trypan blue cell death assay was performed on mouse CT-26 cells to investigate localized photothermal destruction of the cancer cells. High-magnification optical microscopy images of the cells dispersed in the PSi/EtOH:PEG solution given the laser treatment is shown in Figure 4. The cells were first treated with a 0.7 g/L PSi/EtOH:PEG solution. Next, the cells were exposed to laser and then stained by trypan blue dye to examine cell damage. The color of the dead cells usually turns black after staining treatment. It can be seen in Figure 4 that more than 60% of the cells irradiated with NIR laser have turned black in color, indicating cell death.


Porous silicon nanoparticles for cancer photothermotherapy.

Hong C, Lee J, Zheng H, Hong SS, Lee C - Nanoscale Res Lett (2011)

Trypan blue staining. Optical microscopic images of the CT-26 cells treated with a PSi/EtOH:PEG solution (0.7 g/L) followed by NIR laser treatments (for 20 min at 600 mW/cm2) 4 times for 2 min each time with a time interval of 2 min. The cells were stained using trypan blue dye after the NIR laser treatments to examine cell damage. The cells indicated by arrows are some examples of dead cells turned blue after staining.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211409&req=5

Figure 4: Trypan blue staining. Optical microscopic images of the CT-26 cells treated with a PSi/EtOH:PEG solution (0.7 g/L) followed by NIR laser treatments (for 20 min at 600 mW/cm2) 4 times for 2 min each time with a time interval of 2 min. The cells were stained using trypan blue dye after the NIR laser treatments to examine cell damage. The cells indicated by arrows are some examples of dead cells turned blue after staining.
Mentions: To determine whether the effect of PSi nanoparticles under the NIR laser irradiation on cells was cytotoxic, trypan blue cell death assay was performed on mouse CT-26 cells to investigate localized photothermal destruction of the cancer cells. High-magnification optical microscopy images of the cells dispersed in the PSi/EtOH:PEG solution given the laser treatment is shown in Figure 4. The cells were first treated with a 0.7 g/L PSi/EtOH:PEG solution. Next, the cells were exposed to laser and then stained by trypan blue dye to examine cell damage. The color of the dead cells usually turns black after staining treatment. It can be seen in Figure 4 that more than 60% of the cells irradiated with NIR laser have turned black in color, indicating cell death.

Bottom Line: Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis.Tumors have not recurred at all in the PSi/NIR treatment groups thereafter.Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Materials Science and Engineering, Inha University, 253 Yonghyeon-dong, Incheon, 402-751, Republic of Korea. cmlee@inha.ac.kr.

ABSTRACT
The in vitro cell tests and in vivo animal tests were performed to investigate the feasibility of the photothermal therapy based on porous silicon (PSi) in combination with near-infrared (NIR) laser. According to the Annexin V- fluorescein isothiocyanate Apoptosis assay test results, the untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%. Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis. The in vivo animal test results showed that the Murine colon carcinoma (CT-26) tumors were completely resorbed without nearly giving damage to surrounding healthy tissue within 5 days of PSi and NIR laser treatment. Tumors have not recurred at all in the PSi/NIR treatment groups thereafter. Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

No MeSH data available.


Related in: MedlinePlus