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Porous silicon nanoparticles for cancer photothermotherapy.

Hong C, Lee J, Zheng H, Hong SS, Lee C - Nanoscale Res Lett (2011)

Bottom Line: Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis.Tumors have not recurred at all in the PSi/NIR treatment groups thereafter.Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Materials Science and Engineering, Inha University, 253 Yonghyeon-dong, Incheon, 402-751, Republic of Korea. cmlee@inha.ac.kr.

ABSTRACT
The in vitro cell tests and in vivo animal tests were performed to investigate the feasibility of the photothermal therapy based on porous silicon (PSi) in combination with near-infrared (NIR) laser. According to the Annexin V- fluorescein isothiocyanate Apoptosis assay test results, the untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%. Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis. The in vivo animal test results showed that the Murine colon carcinoma (CT-26) tumors were completely resorbed without nearly giving damage to surrounding healthy tissue within 5 days of PSi and NIR laser treatment. Tumors have not recurred at all in the PSi/NIR treatment groups thereafter. Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

No MeSH data available.


Related in: MedlinePlus

Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor laser treatment (control), (b) PSi treatment only, (c) NIR laser treatment only (for 20 min at 600 mW/cm2), and (d) PSi treatment (PSi concentration = 0.7 g/L) followed by laser treatment (for 20 min at 600 mW/cm2). (e) Summary of the Annexin V-FITC Apoptosis assay results showing the percentages of cell death modes: necrosis, late apoptosis, early apoptosis, and live cell.
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Figure 3: Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor laser treatment (control), (b) PSi treatment only, (c) NIR laser treatment only (for 20 min at 600 mW/cm2), and (d) PSi treatment (PSi concentration = 0.7 g/L) followed by laser treatment (for 20 min at 600 mW/cm2). (e) Summary of the Annexin V-FITC Apoptosis assay results showing the percentages of cell death modes: necrosis, late apoptosis, early apoptosis, and live cell.

Mentions: The fluorescent-activated cell sorter (FACS) flow cytometry profiles (Figure 3a-d) obtained as a result of Annexin V-FITC Apoptosis assay represent Annexin V-FITC staining in X-axis and PI in Y-axis. The four sections of the quadrant in each profile from the upper left in a clockwise direction represent necrosis, late apoptosis, early apoptosis, and live cell, respectively. Of these four kinds of cell modes, necrosis and late apoptosis are usually considered as cell death. The Annexin V-FITC Apoptosis assay results in (Figure 3a-d) are summarized in Figure 3e. The untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%, implying that most cells are killed. The group treated with both PSi and NIR laser shows substantially higher cell death (necrosis + late apoptosis) rate than those not given both treatments. It can also be seen in Figure 3e that the cell deaths are mostly due to necrosis but partly due to late apoptosis. This in vitro cell test result suggests that only combination of PSi and NIR laser treatments can kill cells. The viability of 74.4% for the cells treated with PSi but not with NIR irradiation seems to be low, suggesting that PSi is somewhat toxic. This toxicity of PSi may originate from HF residues on the surfaces of the PSi nanoparticles due to incomplete washing. More effort should be made to remove all the HF residues during the course of PSi nanoparticles preparation.


Porous silicon nanoparticles for cancer photothermotherapy.

Hong C, Lee J, Zheng H, Hong SS, Lee C - Nanoscale Res Lett (2011)

Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor laser treatment (control), (b) PSi treatment only, (c) NIR laser treatment only (for 20 min at 600 mW/cm2), and (d) PSi treatment (PSi concentration = 0.7 g/L) followed by laser treatment (for 20 min at 600 mW/cm2). (e) Summary of the Annexin V-FITC Apoptosis assay results showing the percentages of cell death modes: necrosis, late apoptosis, early apoptosis, and live cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211409&req=5

Figure 3: Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor laser treatment (control), (b) PSi treatment only, (c) NIR laser treatment only (for 20 min at 600 mW/cm2), and (d) PSi treatment (PSi concentration = 0.7 g/L) followed by laser treatment (for 20 min at 600 mW/cm2). (e) Summary of the Annexin V-FITC Apoptosis assay results showing the percentages of cell death modes: necrosis, late apoptosis, early apoptosis, and live cell.
Mentions: The fluorescent-activated cell sorter (FACS) flow cytometry profiles (Figure 3a-d) obtained as a result of Annexin V-FITC Apoptosis assay represent Annexin V-FITC staining in X-axis and PI in Y-axis. The four sections of the quadrant in each profile from the upper left in a clockwise direction represent necrosis, late apoptosis, early apoptosis, and live cell, respectively. Of these four kinds of cell modes, necrosis and late apoptosis are usually considered as cell death. The Annexin V-FITC Apoptosis assay results in (Figure 3a-d) are summarized in Figure 3e. The untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%, implying that most cells are killed. The group treated with both PSi and NIR laser shows substantially higher cell death (necrosis + late apoptosis) rate than those not given both treatments. It can also be seen in Figure 3e that the cell deaths are mostly due to necrosis but partly due to late apoptosis. This in vitro cell test result suggests that only combination of PSi and NIR laser treatments can kill cells. The viability of 74.4% for the cells treated with PSi but not with NIR irradiation seems to be low, suggesting that PSi is somewhat toxic. This toxicity of PSi may originate from HF residues on the surfaces of the PSi nanoparticles due to incomplete washing. More effort should be made to remove all the HF residues during the course of PSi nanoparticles preparation.

Bottom Line: Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis.Tumors have not recurred at all in the PSi/NIR treatment groups thereafter.Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Materials Science and Engineering, Inha University, 253 Yonghyeon-dong, Incheon, 402-751, Republic of Korea. cmlee@inha.ac.kr.

ABSTRACT
The in vitro cell tests and in vivo animal tests were performed to investigate the feasibility of the photothermal therapy based on porous silicon (PSi) in combination with near-infrared (NIR) laser. According to the Annexin V- fluorescein isothiocyanate Apoptosis assay test results, the untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%. Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis. The in vivo animal test results showed that the Murine colon carcinoma (CT-26) tumors were completely resorbed without nearly giving damage to surrounding healthy tissue within 5 days of PSi and NIR laser treatment. Tumors have not recurred at all in the PSi/NIR treatment groups thereafter. Both the in vitro cell test and in vivo animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.

No MeSH data available.


Related in: MedlinePlus