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Sustained release of VEGF from PLGA nanoparticles embedded thermo-sensitive hydrogel in full-thickness porcine bladder acellular matrix.

Geng H, Song H, Qi J, Cui D - Nanoscale Res Lett (2011)

Bottom Line: We identified and optimized various formulations and process parameters to get the preferred particle size, entrapment, and polydispersibility of the VEGF-NPs, and incorporated the VEGF-NPs into the (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (Pluronic®) F127 to achieve the preferred VEGF-NPs thermo-sensitive gel system.Furthermore, the system can create a satisfactory tissue-compatible environment and an effective VEGF-sustained release approach.In conclusion, a novel VEGF-loaded PLGA NPs-embedded thermo-sensitive hydrogel in porcine BAMA system is successfully prepared, to provide a promising way for deficient bladder reconstruction therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Bio-Nano Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China. songhua@sjtu.edu.cn.

ABSTRACT
We fabricated a novel vascular endothelial growth factor (VEGF)-loaded poly(lactic-co-glycolic acid) (PLGA)-nanoparticles (NPs)-embedded thermo-sensitive hydrogel in porcine bladder acellular matrix allograft (BAMA) system, which is designed for achieving a sustained release of VEGF protein, and embedding the protein carrier into the BAMA. We identified and optimized various formulations and process parameters to get the preferred particle size, entrapment, and polydispersibility of the VEGF-NPs, and incorporated the VEGF-NPs into the (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (Pluronic®) F127 to achieve the preferred VEGF-NPs thermo-sensitive gel system. Then the thermal behavior of the system was proven by in vitro and in vivo study, and the kinetic-sustained release profile of the system embedded in porcine bladder acellular matrix was investigated. Results indicated that the bioactivity of the encapsulated VEGF released from the NPs was reserved, and the VEGF-NPs thermo-sensitive gel system can achieve sol-gel transmission successfully at appropriate temperature. Furthermore, the system can create a satisfactory tissue-compatible environment and an effective VEGF-sustained release approach. In conclusion, a novel VEGF-loaded PLGA NPs-embedded thermo-sensitive hydrogel in porcine BAMA system is successfully prepared, to provide a promising way for deficient bladder reconstruction therapy.

No MeSH data available.


Related in: MedlinePlus

In vivo thermal behavior fluorescence imaging test using physiologically normal nude mice: (a) nude mouse treated with QDs-NPs-F127 gel, (b) nude mouse treated with QDs-NPs, and (c) nude mouse treated with QDs-physiological saline solution. All mice were treated with aliquots QDs dosage of 2 mg/kg via subcutaneous injection. Intensity bar shows the fluorescence intensity level.
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Figure 5: In vivo thermal behavior fluorescence imaging test using physiologically normal nude mice: (a) nude mouse treated with QDs-NPs-F127 gel, (b) nude mouse treated with QDs-NPs, and (c) nude mouse treated with QDs-physiological saline solution. All mice were treated with aliquots QDs dosage of 2 mg/kg via subcutaneous injection. Intensity bar shows the fluorescence intensity level.

Mentions: Figure 5 exhibits the typical fluorescence images of different healthy group mice from 10-min to 24-h post-injection. Figure 5a represents the image of the mice with QDs-NPs-F127 gel treatment on the right leg, and bright fluorescence signal was observed at10 min; after 24 h, the intensity of the fluorescence signal did not vanish. Obviously, this may possibly be due to the rapid sol-gel transmission of the QDs-NPs-F127 gel. Furthermore, the shape of the injectant below the skin of the mouse was maintained in smooth and clear condition after 24 h, and the tissues around the injectant did not induce any inflammatory reaction, representing the superior biocompatibility of the QDs-NPs-F127 gel. Figure 5b represents the fluorescence images of the mice with QDs-NPs treatment; the images show that the fluorescence signal was aggregated and weakened rapidly after injection, the solvent of the injectant was rapidly absorbed simultaneously, and the NPs were compressed and degraded in accelerated manner. Figure 5c represents the images of the mice with QDs-physiological saline solution, while a curious inflammatory reaction, the swelling, and distension at the administration site were observed after 24 h of post-injection, and this may be attributed to the serious toxicity of the CdTe QDs.


Sustained release of VEGF from PLGA nanoparticles embedded thermo-sensitive hydrogel in full-thickness porcine bladder acellular matrix.

Geng H, Song H, Qi J, Cui D - Nanoscale Res Lett (2011)

In vivo thermal behavior fluorescence imaging test using physiologically normal nude mice: (a) nude mouse treated with QDs-NPs-F127 gel, (b) nude mouse treated with QDs-NPs, and (c) nude mouse treated with QDs-physiological saline solution. All mice were treated with aliquots QDs dosage of 2 mg/kg via subcutaneous injection. Intensity bar shows the fluorescence intensity level.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211399&req=5

Figure 5: In vivo thermal behavior fluorescence imaging test using physiologically normal nude mice: (a) nude mouse treated with QDs-NPs-F127 gel, (b) nude mouse treated with QDs-NPs, and (c) nude mouse treated with QDs-physiological saline solution. All mice were treated with aliquots QDs dosage of 2 mg/kg via subcutaneous injection. Intensity bar shows the fluorescence intensity level.
Mentions: Figure 5 exhibits the typical fluorescence images of different healthy group mice from 10-min to 24-h post-injection. Figure 5a represents the image of the mice with QDs-NPs-F127 gel treatment on the right leg, and bright fluorescence signal was observed at10 min; after 24 h, the intensity of the fluorescence signal did not vanish. Obviously, this may possibly be due to the rapid sol-gel transmission of the QDs-NPs-F127 gel. Furthermore, the shape of the injectant below the skin of the mouse was maintained in smooth and clear condition after 24 h, and the tissues around the injectant did not induce any inflammatory reaction, representing the superior biocompatibility of the QDs-NPs-F127 gel. Figure 5b represents the fluorescence images of the mice with QDs-NPs treatment; the images show that the fluorescence signal was aggregated and weakened rapidly after injection, the solvent of the injectant was rapidly absorbed simultaneously, and the NPs were compressed and degraded in accelerated manner. Figure 5c represents the images of the mice with QDs-physiological saline solution, while a curious inflammatory reaction, the swelling, and distension at the administration site were observed after 24 h of post-injection, and this may be attributed to the serious toxicity of the CdTe QDs.

Bottom Line: We identified and optimized various formulations and process parameters to get the preferred particle size, entrapment, and polydispersibility of the VEGF-NPs, and incorporated the VEGF-NPs into the (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (Pluronic®) F127 to achieve the preferred VEGF-NPs thermo-sensitive gel system.Furthermore, the system can create a satisfactory tissue-compatible environment and an effective VEGF-sustained release approach.In conclusion, a novel VEGF-loaded PLGA NPs-embedded thermo-sensitive hydrogel in porcine BAMA system is successfully prepared, to provide a promising way for deficient bladder reconstruction therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Bio-Nano Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China. songhua@sjtu.edu.cn.

ABSTRACT
We fabricated a novel vascular endothelial growth factor (VEGF)-loaded poly(lactic-co-glycolic acid) (PLGA)-nanoparticles (NPs)-embedded thermo-sensitive hydrogel in porcine bladder acellular matrix allograft (BAMA) system, which is designed for achieving a sustained release of VEGF protein, and embedding the protein carrier into the BAMA. We identified and optimized various formulations and process parameters to get the preferred particle size, entrapment, and polydispersibility of the VEGF-NPs, and incorporated the VEGF-NPs into the (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (Pluronic®) F127 to achieve the preferred VEGF-NPs thermo-sensitive gel system. Then the thermal behavior of the system was proven by in vitro and in vivo study, and the kinetic-sustained release profile of the system embedded in porcine bladder acellular matrix was investigated. Results indicated that the bioactivity of the encapsulated VEGF released from the NPs was reserved, and the VEGF-NPs thermo-sensitive gel system can achieve sol-gel transmission successfully at appropriate temperature. Furthermore, the system can create a satisfactory tissue-compatible environment and an effective VEGF-sustained release approach. In conclusion, a novel VEGF-loaded PLGA NPs-embedded thermo-sensitive hydrogel in porcine BAMA system is successfully prepared, to provide a promising way for deficient bladder reconstruction therapy.

No MeSH data available.


Related in: MedlinePlus