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Sustained release of VEGF from PLGA nanoparticles embedded thermo-sensitive hydrogel in full-thickness porcine bladder acellular matrix.

Geng H, Song H, Qi J, Cui D - Nanoscale Res Lett (2011)

Bottom Line: We identified and optimized various formulations and process parameters to get the preferred particle size, entrapment, and polydispersibility of the VEGF-NPs, and incorporated the VEGF-NPs into the (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (Pluronic®) F127 to achieve the preferred VEGF-NPs thermo-sensitive gel system.Furthermore, the system can create a satisfactory tissue-compatible environment and an effective VEGF-sustained release approach.In conclusion, a novel VEGF-loaded PLGA NPs-embedded thermo-sensitive hydrogel in porcine BAMA system is successfully prepared, to provide a promising way for deficient bladder reconstruction therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Bio-Nano Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China. songhua@sjtu.edu.cn.

ABSTRACT
We fabricated a novel vascular endothelial growth factor (VEGF)-loaded poly(lactic-co-glycolic acid) (PLGA)-nanoparticles (NPs)-embedded thermo-sensitive hydrogel in porcine bladder acellular matrix allograft (BAMA) system, which is designed for achieving a sustained release of VEGF protein, and embedding the protein carrier into the BAMA. We identified and optimized various formulations and process parameters to get the preferred particle size, entrapment, and polydispersibility of the VEGF-NPs, and incorporated the VEGF-NPs into the (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (Pluronic®) F127 to achieve the preferred VEGF-NPs thermo-sensitive gel system. Then the thermal behavior of the system was proven by in vitro and in vivo study, and the kinetic-sustained release profile of the system embedded in porcine bladder acellular matrix was investigated. Results indicated that the bioactivity of the encapsulated VEGF released from the NPs was reserved, and the VEGF-NPs thermo-sensitive gel system can achieve sol-gel transmission successfully at appropriate temperature. Furthermore, the system can create a satisfactory tissue-compatible environment and an effective VEGF-sustained release approach. In conclusion, a novel VEGF-loaded PLGA NPs-embedded thermo-sensitive hydrogel in porcine BAMA system is successfully prepared, to provide a promising way for deficient bladder reconstruction therapy.

No MeSH data available.


Related in: MedlinePlus

Proliferation of HUVEC cells was induced by 10 ng/mL free-VEGF, or 20 ng/mL free-VEGF, or 10 ng/mL VEGF in NPs, or 20 ng/mL VEGF in NPs, or non-loaded NPs (NL-NPs) at the same concentration of PLGA with the application described above, and compared to culture medium alone (control) for 1-5 days. Y-axis represents fold increase versus control. Asterisk represents P < 0.05 and double asterisk represents P < 0.01.
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Figure 2: Proliferation of HUVEC cells was induced by 10 ng/mL free-VEGF, or 20 ng/mL free-VEGF, or 10 ng/mL VEGF in NPs, or 20 ng/mL VEGF in NPs, or non-loaded NPs (NL-NPs) at the same concentration of PLGA with the application described above, and compared to culture medium alone (control) for 1-5 days. Y-axis represents fold increase versus control. Asterisk represents P < 0.05 and double asterisk represents P < 0.01.

Mentions: The bioactivity of the encapsulated VEGF released from the NPs was examined by determining its capacity to induce proliferation of endothelial cells (HUVEC) (Figure 2). VEGF-NPs (10 or 20 ng/mL) induced a 2-2.5-fold increase in proliferation of HUVEC in comparison with control (no VEGF) or non-loaded NPs (NL-NPs) after 3 days in culture (P < 0.01). This increase was similar to that observed when HUVEC cells were cultured with addition of free-VEGF at doses of 10 or 20 ng/mL. The results show NL-NPs caused little reduction in cell viability compared with the control, but there was not any significant statistical difference between them, indicating that NL-NPs were better tolerated at the experiment's concentration. Furthermore, similar levels of stimulation in the HUVEC cells treated either with the free-VEGF or the VEGF-NPs were detected, confirming that the process of encapsulation does not affect negatively VEGF biological activity significantly.


Sustained release of VEGF from PLGA nanoparticles embedded thermo-sensitive hydrogel in full-thickness porcine bladder acellular matrix.

Geng H, Song H, Qi J, Cui D - Nanoscale Res Lett (2011)

Proliferation of HUVEC cells was induced by 10 ng/mL free-VEGF, or 20 ng/mL free-VEGF, or 10 ng/mL VEGF in NPs, or 20 ng/mL VEGF in NPs, or non-loaded NPs (NL-NPs) at the same concentration of PLGA with the application described above, and compared to culture medium alone (control) for 1-5 days. Y-axis represents fold increase versus control. Asterisk represents P < 0.05 and double asterisk represents P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211399&req=5

Figure 2: Proliferation of HUVEC cells was induced by 10 ng/mL free-VEGF, or 20 ng/mL free-VEGF, or 10 ng/mL VEGF in NPs, or 20 ng/mL VEGF in NPs, or non-loaded NPs (NL-NPs) at the same concentration of PLGA with the application described above, and compared to culture medium alone (control) for 1-5 days. Y-axis represents fold increase versus control. Asterisk represents P < 0.05 and double asterisk represents P < 0.01.
Mentions: The bioactivity of the encapsulated VEGF released from the NPs was examined by determining its capacity to induce proliferation of endothelial cells (HUVEC) (Figure 2). VEGF-NPs (10 or 20 ng/mL) induced a 2-2.5-fold increase in proliferation of HUVEC in comparison with control (no VEGF) or non-loaded NPs (NL-NPs) after 3 days in culture (P < 0.01). This increase was similar to that observed when HUVEC cells were cultured with addition of free-VEGF at doses of 10 or 20 ng/mL. The results show NL-NPs caused little reduction in cell viability compared with the control, but there was not any significant statistical difference between them, indicating that NL-NPs were better tolerated at the experiment's concentration. Furthermore, similar levels of stimulation in the HUVEC cells treated either with the free-VEGF or the VEGF-NPs were detected, confirming that the process of encapsulation does not affect negatively VEGF biological activity significantly.

Bottom Line: We identified and optimized various formulations and process parameters to get the preferred particle size, entrapment, and polydispersibility of the VEGF-NPs, and incorporated the VEGF-NPs into the (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (Pluronic®) F127 to achieve the preferred VEGF-NPs thermo-sensitive gel system.Furthermore, the system can create a satisfactory tissue-compatible environment and an effective VEGF-sustained release approach.In conclusion, a novel VEGF-loaded PLGA NPs-embedded thermo-sensitive hydrogel in porcine BAMA system is successfully prepared, to provide a promising way for deficient bladder reconstruction therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Bio-Nano Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China. songhua@sjtu.edu.cn.

ABSTRACT
We fabricated a novel vascular endothelial growth factor (VEGF)-loaded poly(lactic-co-glycolic acid) (PLGA)-nanoparticles (NPs)-embedded thermo-sensitive hydrogel in porcine bladder acellular matrix allograft (BAMA) system, which is designed for achieving a sustained release of VEGF protein, and embedding the protein carrier into the BAMA. We identified and optimized various formulations and process parameters to get the preferred particle size, entrapment, and polydispersibility of the VEGF-NPs, and incorporated the VEGF-NPs into the (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (Pluronic®) F127 to achieve the preferred VEGF-NPs thermo-sensitive gel system. Then the thermal behavior of the system was proven by in vitro and in vivo study, and the kinetic-sustained release profile of the system embedded in porcine bladder acellular matrix was investigated. Results indicated that the bioactivity of the encapsulated VEGF released from the NPs was reserved, and the VEGF-NPs thermo-sensitive gel system can achieve sol-gel transmission successfully at appropriate temperature. Furthermore, the system can create a satisfactory tissue-compatible environment and an effective VEGF-sustained release approach. In conclusion, a novel VEGF-loaded PLGA NPs-embedded thermo-sensitive hydrogel in porcine BAMA system is successfully prepared, to provide a promising way for deficient bladder reconstruction therapy.

No MeSH data available.


Related in: MedlinePlus