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An antibacterial coating based on a polymer/sol-gel hybrid matrix loaded with silver nanoparticles.

Rivero PJ, Urrutia A, Goicoechea J, Zamarreño CR, Arregui FJ, Matías IR - Nanoscale Res Lett (2011)

Bottom Line: Energy dispersive X-ray (EDX) was also used to confirm the presence of the resulting silver nanoparticles within the thin films.Finally the coatings have been tested in bacterial cultures of genus Lactobacillus plantarum to observe their antibacterial properties.It has been experimentally demonstrated that these silver loaded organic-inorganic hybrid films have a very good antimicrobial behavior against this type of bacteria.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nanostructured Optical Devices Laboratory, Electric and Electronic Engineering Department, Public University of Navarra, Edif, Los Tejos, Campus Arrosadía, 31006, Pamplona, Spain. pedrojose.rivero@unavarra.es.

ABSTRACT
In this work a novel antibacterial surface composed of an organic-inorganic hybrid matrix of tetraorthosilicate and a polyelectrolyte is presented. A precursor solution of tetraethoxysilane (TEOS) and poly(acrylic acid sodium salt) (PAA) was prepared and subsequently thin films were fabricated by the dip-coating technique using glass slides as substrates. This hybrid matrix coating is further loaded with silver nanoparticles using an in situ synthesis route. The morphology and composition of the coatings have been studied using UV-VIS spectroscopy and atomic force microscopy (AFM). Energy dispersive X-ray (EDX) was also used to confirm the presence of the resulting silver nanoparticles within the thin films. Finally the coatings have been tested in bacterial cultures of genus Lactobacillus plantarum to observe their antibacterial properties. It has been experimentally demonstrated that these silver loaded organic-inorganic hybrid films have a very good antimicrobial behavior against this type of bacteria.

No MeSH data available.


Related in: MedlinePlus

UV-VIS absorption. UV-VIS absorption spectra of the coating with different number of loading/reduction.
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Figure 2: UV-VIS absorption. UV-VIS absorption spectra of the coating with different number of loading/reduction.

Mentions: As it has been commented in the previous section, all the coatings showed a highly transparent appearance immediately after the dip-coating fabrication step. The composition of such coatings consisted of an inorganic matrix with a small proportion of an anionic polyelectrolyte embedded within. Such polymeric chains have carboxylic functional groups that act as host sites for the silver cations during the loading immersion step by ion-interchange. Other authors have reported similar approaches to load polymeric thin films with silver ions [35]. The visual aspect of the hybrid thin films remained unaltered after the silver loading immersion, although the silver ions were already present in the coating. However, the thin films showed a dramatic color change when they were immersed into the DMAB reducing solution. Then the samples turned almost instantaneously into a golden-yellowish color. The visual aspect of the film remained highly transparent and smooth, but with the cited color change. Such alteration of the visible absorption spectrum of the samples change is directly related with the Surface Plasmon Resonance (SPR) phenomenon [39,40] typical of gold and silver nanoparticles. In fact, the UV-VIS absorbance spectrum confirms the existence of an absorption peak near 410 nm (Figure 2).


An antibacterial coating based on a polymer/sol-gel hybrid matrix loaded with silver nanoparticles.

Rivero PJ, Urrutia A, Goicoechea J, Zamarreño CR, Arregui FJ, Matías IR - Nanoscale Res Lett (2011)

UV-VIS absorption. UV-VIS absorption spectra of the coating with different number of loading/reduction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211391&req=5

Figure 2: UV-VIS absorption. UV-VIS absorption spectra of the coating with different number of loading/reduction.
Mentions: As it has been commented in the previous section, all the coatings showed a highly transparent appearance immediately after the dip-coating fabrication step. The composition of such coatings consisted of an inorganic matrix with a small proportion of an anionic polyelectrolyte embedded within. Such polymeric chains have carboxylic functional groups that act as host sites for the silver cations during the loading immersion step by ion-interchange. Other authors have reported similar approaches to load polymeric thin films with silver ions [35]. The visual aspect of the hybrid thin films remained unaltered after the silver loading immersion, although the silver ions were already present in the coating. However, the thin films showed a dramatic color change when they were immersed into the DMAB reducing solution. Then the samples turned almost instantaneously into a golden-yellowish color. The visual aspect of the film remained highly transparent and smooth, but with the cited color change. Such alteration of the visible absorption spectrum of the samples change is directly related with the Surface Plasmon Resonance (SPR) phenomenon [39,40] typical of gold and silver nanoparticles. In fact, the UV-VIS absorbance spectrum confirms the existence of an absorption peak near 410 nm (Figure 2).

Bottom Line: Energy dispersive X-ray (EDX) was also used to confirm the presence of the resulting silver nanoparticles within the thin films.Finally the coatings have been tested in bacterial cultures of genus Lactobacillus plantarum to observe their antibacterial properties.It has been experimentally demonstrated that these silver loaded organic-inorganic hybrid films have a very good antimicrobial behavior against this type of bacteria.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nanostructured Optical Devices Laboratory, Electric and Electronic Engineering Department, Public University of Navarra, Edif, Los Tejos, Campus Arrosadía, 31006, Pamplona, Spain. pedrojose.rivero@unavarra.es.

ABSTRACT
In this work a novel antibacterial surface composed of an organic-inorganic hybrid matrix of tetraorthosilicate and a polyelectrolyte is presented. A precursor solution of tetraethoxysilane (TEOS) and poly(acrylic acid sodium salt) (PAA) was prepared and subsequently thin films were fabricated by the dip-coating technique using glass slides as substrates. This hybrid matrix coating is further loaded with silver nanoparticles using an in situ synthesis route. The morphology and composition of the coatings have been studied using UV-VIS spectroscopy and atomic force microscopy (AFM). Energy dispersive X-ray (EDX) was also used to confirm the presence of the resulting silver nanoparticles within the thin films. Finally the coatings have been tested in bacterial cultures of genus Lactobacillus plantarum to observe their antibacterial properties. It has been experimentally demonstrated that these silver loaded organic-inorganic hybrid films have a very good antimicrobial behavior against this type of bacteria.

No MeSH data available.


Related in: MedlinePlus