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Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles.

Ruan J, Wang K, Song H, Xu X, Ji J, Cui D - Nanoscale Res Lett (2011)

Bottom Line: HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days.FMNPs primarily accumulated in those organs such as lung, liver, and spleen.The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. dxcui@sjtu.edu.cn.

ABSTRACT
Fluorescent magnetic nanoparticles exhibit great application prospects in biomedical engineering. Herein, we reported the effects of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles (FMNPs) on human embryonic kidney 293 (HEK293) cells and mice with the aim of investigating their biocompatibility. FMNPs with 150 nm in diameter were prepared, and characterized by high-resolution transmission electron microscopy and photoluminescence (PL) spectra and magnetometer. HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days. Cell viability and adhesion ability were analyzed by CCK8 method and Western blotting. 30 mice were randomly divided into three groups, and were, respectively, injected via tail vein with 20, 60, and 100 μg FMNPs, and then were, respectively, raised for 1, 7, and 30 days, then their lifespan, important organs, and blood biochemical parameters were analyzed. Results show that the prepared water-soluble FMNPs had high fluorescent and magnetic properties, less than 50 μg/ml of FMNPs exhibited good biocompatibility to HEK293 cells, the cell viability, and adhesion ability were similar to the control HEK293 cells. FMNPs primarily accumulated in those organs such as lung, liver, and spleen. Lung exposed to FMNPs displayed a dose-dependent inflammatory response, blood biochemical parameters such as white blood cell count (WBC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), displayed significant increase when the FMNPs were injected into mice at dose of 100μg. In conclusion, FMNPs exhibit good biocompatibility to cells under the dose of less than 50 μg/ml, and to mice under the dose of less than 2mg/kg body weight. The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

No MeSH data available.


Related in: MedlinePlus

Effects of FMNPs on cell adhesion ability and adhesive proteins. (a) The adhesion ability of HEK293 cells treated with FMNPs. (b) Western blot analysis of the effect of FMNPs on the expressions of cyclin D3, laminin, FAK, fibronectin, and β-actin. Lanes 1, 2, 3, and 4 are the protein expressions at FMNPs concentrations of 0, 20, 50, and 100 μg/ml, respectively.
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Figure 7: Effects of FMNPs on cell adhesion ability and adhesive proteins. (a) The adhesion ability of HEK293 cells treated with FMNPs. (b) Western blot analysis of the effect of FMNPs on the expressions of cyclin D3, laminin, FAK, fibronectin, and β-actin. Lanes 1, 2, 3, and 4 are the protein expressions at FMNPs concentrations of 0, 20, 50, and 100 μg/ml, respectively.

Mentions: The adhesive ability of FMNPs-treated HEK293 cells were evaluated by centrifugation method. As shown in Figure 7a, the adhesion ability of HEK293 cells decreased gradually as the amount of FMNPs and culture days increased. As shown in Figure 7b, Western blotting analysis showed that the adhesive proteins such as laminin, fibronectin, FAK, and cell cycle protein cyclin D3 exhibited gradual down-regulation expression as the concentration of FMNPs increased. The β-actin protein expression as the control, kept unchanged in each case. These results showed that FMNPs can induce adhesion-associated proteins exhibit down-regulation expression, leading to thinning of cell basement membrane and decrease of the cell adhesion ability, finally resulting in cellular apoptosis or death.


Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles.

Ruan J, Wang K, Song H, Xu X, Ji J, Cui D - Nanoscale Res Lett (2011)

Effects of FMNPs on cell adhesion ability and adhesive proteins. (a) The adhesion ability of HEK293 cells treated with FMNPs. (b) Western blot analysis of the effect of FMNPs on the expressions of cyclin D3, laminin, FAK, fibronectin, and β-actin. Lanes 1, 2, 3, and 4 are the protein expressions at FMNPs concentrations of 0, 20, 50, and 100 μg/ml, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211365&req=5

Figure 7: Effects of FMNPs on cell adhesion ability and adhesive proteins. (a) The adhesion ability of HEK293 cells treated with FMNPs. (b) Western blot analysis of the effect of FMNPs on the expressions of cyclin D3, laminin, FAK, fibronectin, and β-actin. Lanes 1, 2, 3, and 4 are the protein expressions at FMNPs concentrations of 0, 20, 50, and 100 μg/ml, respectively.
Mentions: The adhesive ability of FMNPs-treated HEK293 cells were evaluated by centrifugation method. As shown in Figure 7a, the adhesion ability of HEK293 cells decreased gradually as the amount of FMNPs and culture days increased. As shown in Figure 7b, Western blotting analysis showed that the adhesive proteins such as laminin, fibronectin, FAK, and cell cycle protein cyclin D3 exhibited gradual down-regulation expression as the concentration of FMNPs increased. The β-actin protein expression as the control, kept unchanged in each case. These results showed that FMNPs can induce adhesion-associated proteins exhibit down-regulation expression, leading to thinning of cell basement membrane and decrease of the cell adhesion ability, finally resulting in cellular apoptosis or death.

Bottom Line: HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days.FMNPs primarily accumulated in those organs such as lung, liver, and spleen.The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. dxcui@sjtu.edu.cn.

ABSTRACT
Fluorescent magnetic nanoparticles exhibit great application prospects in biomedical engineering. Herein, we reported the effects of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles (FMNPs) on human embryonic kidney 293 (HEK293) cells and mice with the aim of investigating their biocompatibility. FMNPs with 150 nm in diameter were prepared, and characterized by high-resolution transmission electron microscopy and photoluminescence (PL) spectra and magnetometer. HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days. Cell viability and adhesion ability were analyzed by CCK8 method and Western blotting. 30 mice were randomly divided into three groups, and were, respectively, injected via tail vein with 20, 60, and 100 μg FMNPs, and then were, respectively, raised for 1, 7, and 30 days, then their lifespan, important organs, and blood biochemical parameters were analyzed. Results show that the prepared water-soluble FMNPs had high fluorescent and magnetic properties, less than 50 μg/ml of FMNPs exhibited good biocompatibility to HEK293 cells, the cell viability, and adhesion ability were similar to the control HEK293 cells. FMNPs primarily accumulated in those organs such as lung, liver, and spleen. Lung exposed to FMNPs displayed a dose-dependent inflammatory response, blood biochemical parameters such as white blood cell count (WBC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), displayed significant increase when the FMNPs were injected into mice at dose of 100μg. In conclusion, FMNPs exhibit good biocompatibility to cells under the dose of less than 50 μg/ml, and to mice under the dose of less than 2mg/kg body weight. The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

No MeSH data available.


Related in: MedlinePlus