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Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles.

Ruan J, Wang K, Song H, Xu X, Ji J, Cui D - Nanoscale Res Lett (2011)

Bottom Line: HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days.FMNPs primarily accumulated in those organs such as lung, liver, and spleen.The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. dxcui@sjtu.edu.cn.

ABSTRACT
Fluorescent magnetic nanoparticles exhibit great application prospects in biomedical engineering. Herein, we reported the effects of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles (FMNPs) on human embryonic kidney 293 (HEK293) cells and mice with the aim of investigating their biocompatibility. FMNPs with 150 nm in diameter were prepared, and characterized by high-resolution transmission electron microscopy and photoluminescence (PL) spectra and magnetometer. HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days. Cell viability and adhesion ability were analyzed by CCK8 method and Western blotting. 30 mice were randomly divided into three groups, and were, respectively, injected via tail vein with 20, 60, and 100 μg FMNPs, and then were, respectively, raised for 1, 7, and 30 days, then their lifespan, important organs, and blood biochemical parameters were analyzed. Results show that the prepared water-soluble FMNPs had high fluorescent and magnetic properties, less than 50 μg/ml of FMNPs exhibited good biocompatibility to HEK293 cells, the cell viability, and adhesion ability were similar to the control HEK293 cells. FMNPs primarily accumulated in those organs such as lung, liver, and spleen. Lung exposed to FMNPs displayed a dose-dependent inflammatory response, blood biochemical parameters such as white blood cell count (WBC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), displayed significant increase when the FMNPs were injected into mice at dose of 100μg. In conclusion, FMNPs exhibit good biocompatibility to cells under the dose of less than 50 μg/ml, and to mice under the dose of less than 2mg/kg body weight. The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

No MeSH data available.


Related in: MedlinePlus

Effect of FMNPs on survival rate of HEK293 cells, HEK293 cells viability measured by CCK8 assay and the percentage of cell viability was calculated as a ratio of OD of FMNPs-treated cells and control cells.
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Figure 6: Effect of FMNPs on survival rate of HEK293 cells, HEK293 cells viability measured by CCK8 assay and the percentage of cell viability was calculated as a ratio of OD of FMNPs-treated cells and control cells.

Mentions: Regarding the effects of FMNPs on HEK 293 cells, as shown in Figure 6, FMNPs affected the growth of HEK293 cells in time- and dose-dependent means, similar to our previous report [5,55]. FMNPs of 20 μg/ml in medium exhibited no toxicity to cells, the cell survival rate increased with the culture days increased. When the dose of FMNPs in medium reach or overrun 50 μg/ml, FMNPs exhibited low cytotoxicity to HEK 293 cells, the cell growth became slow. When the dose of FMNPs reach or overrun 100 μg/ml in medium, cell survival rate significantly decreased as the culture days increased. Thus, we consider that FMNPs exhibited good biocompatibility to HEK 293 cells within the dose of 50 μg/ml in medium.


Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles.

Ruan J, Wang K, Song H, Xu X, Ji J, Cui D - Nanoscale Res Lett (2011)

Effect of FMNPs on survival rate of HEK293 cells, HEK293 cells viability measured by CCK8 assay and the percentage of cell viability was calculated as a ratio of OD of FMNPs-treated cells and control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211365&req=5

Figure 6: Effect of FMNPs on survival rate of HEK293 cells, HEK293 cells viability measured by CCK8 assay and the percentage of cell viability was calculated as a ratio of OD of FMNPs-treated cells and control cells.
Mentions: Regarding the effects of FMNPs on HEK 293 cells, as shown in Figure 6, FMNPs affected the growth of HEK293 cells in time- and dose-dependent means, similar to our previous report [5,55]. FMNPs of 20 μg/ml in medium exhibited no toxicity to cells, the cell survival rate increased with the culture days increased. When the dose of FMNPs in medium reach or overrun 50 μg/ml, FMNPs exhibited low cytotoxicity to HEK 293 cells, the cell growth became slow. When the dose of FMNPs reach or overrun 100 μg/ml in medium, cell survival rate significantly decreased as the culture days increased. Thus, we consider that FMNPs exhibited good biocompatibility to HEK 293 cells within the dose of 50 μg/ml in medium.

Bottom Line: HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days.FMNPs primarily accumulated in those organs such as lung, liver, and spleen.The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. dxcui@sjtu.edu.cn.

ABSTRACT
Fluorescent magnetic nanoparticles exhibit great application prospects in biomedical engineering. Herein, we reported the effects of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles (FMNPs) on human embryonic kidney 293 (HEK293) cells and mice with the aim of investigating their biocompatibility. FMNPs with 150 nm in diameter were prepared, and characterized by high-resolution transmission electron microscopy and photoluminescence (PL) spectra and magnetometer. HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days. Cell viability and adhesion ability were analyzed by CCK8 method and Western blotting. 30 mice were randomly divided into three groups, and were, respectively, injected via tail vein with 20, 60, and 100 μg FMNPs, and then were, respectively, raised for 1, 7, and 30 days, then their lifespan, important organs, and blood biochemical parameters were analyzed. Results show that the prepared water-soluble FMNPs had high fluorescent and magnetic properties, less than 50 μg/ml of FMNPs exhibited good biocompatibility to HEK293 cells, the cell viability, and adhesion ability were similar to the control HEK293 cells. FMNPs primarily accumulated in those organs such as lung, liver, and spleen. Lung exposed to FMNPs displayed a dose-dependent inflammatory response, blood biochemical parameters such as white blood cell count (WBC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), displayed significant increase when the FMNPs were injected into mice at dose of 100μg. In conclusion, FMNPs exhibit good biocompatibility to cells under the dose of less than 50 μg/ml, and to mice under the dose of less than 2mg/kg body weight. The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

No MeSH data available.


Related in: MedlinePlus