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Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles.

Ruan J, Wang K, Song H, Xu X, Ji J, Cui D - Nanoscale Res Lett (2011)

Bottom Line: HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days.FMNPs primarily accumulated in those organs such as lung, liver, and spleen.The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. dxcui@sjtu.edu.cn.

ABSTRACT
Fluorescent magnetic nanoparticles exhibit great application prospects in biomedical engineering. Herein, we reported the effects of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles (FMNPs) on human embryonic kidney 293 (HEK293) cells and mice with the aim of investigating their biocompatibility. FMNPs with 150 nm in diameter were prepared, and characterized by high-resolution transmission electron microscopy and photoluminescence (PL) spectra and magnetometer. HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days. Cell viability and adhesion ability were analyzed by CCK8 method and Western blotting. 30 mice were randomly divided into three groups, and were, respectively, injected via tail vein with 20, 60, and 100 μg FMNPs, and then were, respectively, raised for 1, 7, and 30 days, then their lifespan, important organs, and blood biochemical parameters were analyzed. Results show that the prepared water-soluble FMNPs had high fluorescent and magnetic properties, less than 50 μg/ml of FMNPs exhibited good biocompatibility to HEK293 cells, the cell viability, and adhesion ability were similar to the control HEK293 cells. FMNPs primarily accumulated in those organs such as lung, liver, and spleen. Lung exposed to FMNPs displayed a dose-dependent inflammatory response, blood biochemical parameters such as white blood cell count (WBC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), displayed significant increase when the FMNPs were injected into mice at dose of 100μg. In conclusion, FMNPs exhibit good biocompatibility to cells under the dose of less than 50 μg/ml, and to mice under the dose of less than 2mg/kg body weight. The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

No MeSH data available.


Related in: MedlinePlus

Characterization of FMNPs. (a) Ternary phase diagram of W/O reverse micro-emulsion reaction system; (b) SEM image of FMNPs, FMNPs were sphere with 150 nm in diameter; (c) TEM image exhibited that the FMNPs are mono-dispersed, silica shells covered Fe3O4/PS and QDs; and (d) the magnified image of the FMNPs presented a silica-shell thickness of 30 nm on average, and the mean total diameter was 150 nm.
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Figure 1: Characterization of FMNPs. (a) Ternary phase diagram of W/O reverse micro-emulsion reaction system; (b) SEM image of FMNPs, FMNPs were sphere with 150 nm in diameter; (c) TEM image exhibited that the FMNPs are mono-dispersed, silica shells covered Fe3O4/PS and QDs; and (d) the magnified image of the FMNPs presented a silica-shell thickness of 30 nm on average, and the mean total diameter was 150 nm.

Mentions: As shown in Figure 1a, through measuring the conductivity and transparency of reverse micro-emulsion variations [48], we discovered that, under 6:4 of the surfactant (Triton X-100 and N-hexanol) and oil phase (cyclohexane), the micro-emulsion's maximal water solubilization had the wide range, thus, under above reaction condition, we can prepare the ideal FMNPs. Figure 1b showed the SEM images of FMNPs, prepared FMNPs were sphere with the average diameter of 150 nm. As shown in Figure 1c, d, FMNPs are mono-dispersed nano-composites composed of silica shells and Fe3O4/PS and QDs, the silica-shell thickness is almost 30 nm, the whole nanocomposites is almost 150 nm in diameter.


Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles.

Ruan J, Wang K, Song H, Xu X, Ji J, Cui D - Nanoscale Res Lett (2011)

Characterization of FMNPs. (a) Ternary phase diagram of W/O reverse micro-emulsion reaction system; (b) SEM image of FMNPs, FMNPs were sphere with 150 nm in diameter; (c) TEM image exhibited that the FMNPs are mono-dispersed, silica shells covered Fe3O4/PS and QDs; and (d) the magnified image of the FMNPs presented a silica-shell thickness of 30 nm on average, and the mean total diameter was 150 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211365&req=5

Figure 1: Characterization of FMNPs. (a) Ternary phase diagram of W/O reverse micro-emulsion reaction system; (b) SEM image of FMNPs, FMNPs were sphere with 150 nm in diameter; (c) TEM image exhibited that the FMNPs are mono-dispersed, silica shells covered Fe3O4/PS and QDs; and (d) the magnified image of the FMNPs presented a silica-shell thickness of 30 nm on average, and the mean total diameter was 150 nm.
Mentions: As shown in Figure 1a, through measuring the conductivity and transparency of reverse micro-emulsion variations [48], we discovered that, under 6:4 of the surfactant (Triton X-100 and N-hexanol) and oil phase (cyclohexane), the micro-emulsion's maximal water solubilization had the wide range, thus, under above reaction condition, we can prepare the ideal FMNPs. Figure 1b showed the SEM images of FMNPs, prepared FMNPs were sphere with the average diameter of 150 nm. As shown in Figure 1c, d, FMNPs are mono-dispersed nano-composites composed of silica shells and Fe3O4/PS and QDs, the silica-shell thickness is almost 30 nm, the whole nanocomposites is almost 150 nm in diameter.

Bottom Line: HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days.FMNPs primarily accumulated in those organs such as lung, liver, and spleen.The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. dxcui@sjtu.edu.cn.

ABSTRACT
Fluorescent magnetic nanoparticles exhibit great application prospects in biomedical engineering. Herein, we reported the effects of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles (FMNPs) on human embryonic kidney 293 (HEK293) cells and mice with the aim of investigating their biocompatibility. FMNPs with 150 nm in diameter were prepared, and characterized by high-resolution transmission electron microscopy and photoluminescence (PL) spectra and magnetometer. HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days. Cell viability and adhesion ability were analyzed by CCK8 method and Western blotting. 30 mice were randomly divided into three groups, and were, respectively, injected via tail vein with 20, 60, and 100 μg FMNPs, and then were, respectively, raised for 1, 7, and 30 days, then their lifespan, important organs, and blood biochemical parameters were analyzed. Results show that the prepared water-soluble FMNPs had high fluorescent and magnetic properties, less than 50 μg/ml of FMNPs exhibited good biocompatibility to HEK293 cells, the cell viability, and adhesion ability were similar to the control HEK293 cells. FMNPs primarily accumulated in those organs such as lung, liver, and spleen. Lung exposed to FMNPs displayed a dose-dependent inflammatory response, blood biochemical parameters such as white blood cell count (WBC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), displayed significant increase when the FMNPs were injected into mice at dose of 100μg. In conclusion, FMNPs exhibit good biocompatibility to cells under the dose of less than 50 μg/ml, and to mice under the dose of less than 2mg/kg body weight. The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

No MeSH data available.


Related in: MedlinePlus