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Novel Strategy to Fabricate PLA/Au Nanocomposites as an Efficient Drug Carrier for Human Leukemia Cells in Vitro

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ABSTRACT

Poly (lactic acid) (PLA) polymer has the promising applications in the biomedical field because of its biodegradability and safe elimination. In this study, we have explored the bio-application of new nanocomposites composed with PLA nanofibers and Au nanoparticles as the potential drug carrier for an efficient drug delivery in target cancer cells. The results demonstrated that the anticancer drug daunorubicin could be efficiently self-assembled on the surface of PLA/Au nanocomposites and the synergistic enhancement of PLA/Au nanocomposites conjugated with daunorubicin into drug-sensitive K562 and drug-resistant leukemia K562/AO2 cells could be obviously observed by MTT assay and confocal fluorescence microscopy studies. These observations suggest that the new nanocomposites could readily induce daunorubicin to accumulate and uptake in target leukemia cells and increase the drug's cytotoxicity. Especially, the PLA/Au nanocomposites could significantly facilitate the cellular drug absorbtion of daunorubicin into drug-resistant K562/AO2 cells and efficiently inhibit the cancer cell proliferation. This raised the possibility to utilize the PLA/Au nanocomposites as a new effective additive agent to inhibit the drug resistance and thus as a novel strategy to sensitively track the respective cancer cells.

No MeSH data available.


Related in: MedlinePlus

Optical microscopy images of leukemia cancer cells. a K562 cells, c K562 treated with DNR, e K562 treated with DNR conjugated with PLA/Au nanocomposites (DNR was 1 × 10-6 M in the above systems); b K562/AO2 cells, d K562/AO2 treated with DNR, f K562/AO2 treated with DNR conjugated with PLA/Au nanocomposites (DNR was 1 × 10-6 M in the above systems).
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Figure 3: Optical microscopy images of leukemia cancer cells. a K562 cells, c K562 treated with DNR, e K562 treated with DNR conjugated with PLA/Au nanocomposites (DNR was 1 × 10-6 M in the above systems); b K562/AO2 cells, d K562/AO2 treated with DNR, f K562/AO2 treated with DNR conjugated with PLA/Au nanocomposites (DNR was 1 × 10-6 M in the above systems).

Mentions: Based on the above observations, the PLA/Au nanocomposites have been further explored as a new potential drug carrier for efficient drug delivery. Initially, the microscopy images of leukemia cancer cells in the absence and presence of PLA/Au nanocomposites have been investigated by optical microscopy. As shown in Figure 3, it is observed that the drug-sensitive leukemia cancer cells K562 and drug-resistant leukemia cancer cells K562/AO2 had the good morphology in the negative control. While K562 cells were cultured with DNR conjugated with PLA/Au nanocomposites, significant morphological changes were detected and more cell death occurred than that of cells treated with DNR alone. In comparison, there were no any morphological changes for drug-resistant leukemia cells K562/AO2 after treated with DNR alone because of the relevant multidrug resistance, as shown in Figure 3b and 3d. After the cells were treated by DNR conjugated with PLA/Au nanocomposites, significant increase in the cell death could be detected. Considering the good biocompatibility of PLA and Au nanomaterials, these observations suggest that the apparent increase in cancer cell death should be attributed to the synergistic function derived from the combination of DNR with PLA/Au nanocomposites. Especially, the PLA/Au nanocomposites–DNR complexes can remarkably facilitate the accumulation of the DNR molecules in the drug-resistant cancer cells and apparently reserve the MDR of K562/AO2.


Novel Strategy to Fabricate PLA/Au Nanocomposites as an Efficient Drug Carrier for Human Leukemia Cells in Vitro
Optical microscopy images of leukemia cancer cells. a K562 cells, c K562 treated with DNR, e K562 treated with DNR conjugated with PLA/Au nanocomposites (DNR was 1 × 10-6 M in the above systems); b K562/AO2 cells, d K562/AO2 treated with DNR, f K562/AO2 treated with DNR conjugated with PLA/Au nanocomposites (DNR was 1 × 10-6 M in the above systems).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211355&req=5

Figure 3: Optical microscopy images of leukemia cancer cells. a K562 cells, c K562 treated with DNR, e K562 treated with DNR conjugated with PLA/Au nanocomposites (DNR was 1 × 10-6 M in the above systems); b K562/AO2 cells, d K562/AO2 treated with DNR, f K562/AO2 treated with DNR conjugated with PLA/Au nanocomposites (DNR was 1 × 10-6 M in the above systems).
Mentions: Based on the above observations, the PLA/Au nanocomposites have been further explored as a new potential drug carrier for efficient drug delivery. Initially, the microscopy images of leukemia cancer cells in the absence and presence of PLA/Au nanocomposites have been investigated by optical microscopy. As shown in Figure 3, it is observed that the drug-sensitive leukemia cancer cells K562 and drug-resistant leukemia cancer cells K562/AO2 had the good morphology in the negative control. While K562 cells were cultured with DNR conjugated with PLA/Au nanocomposites, significant morphological changes were detected and more cell death occurred than that of cells treated with DNR alone. In comparison, there were no any morphological changes for drug-resistant leukemia cells K562/AO2 after treated with DNR alone because of the relevant multidrug resistance, as shown in Figure 3b and 3d. After the cells were treated by DNR conjugated with PLA/Au nanocomposites, significant increase in the cell death could be detected. Considering the good biocompatibility of PLA and Au nanomaterials, these observations suggest that the apparent increase in cancer cell death should be attributed to the synergistic function derived from the combination of DNR with PLA/Au nanocomposites. Especially, the PLA/Au nanocomposites–DNR complexes can remarkably facilitate the accumulation of the DNR molecules in the drug-resistant cancer cells and apparently reserve the MDR of K562/AO2.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Poly (lactic acid) (PLA) polymer has the promising applications in the biomedical field because of its biodegradability and safe elimination. In this study, we have explored the bio-application of new nanocomposites composed with PLA nanofibers and Au nanoparticles as the potential drug carrier for an efficient drug delivery in target cancer cells. The results demonstrated that the anticancer drug daunorubicin could be efficiently self-assembled on the surface of PLA/Au nanocomposites and the synergistic enhancement of PLA/Au nanocomposites conjugated with daunorubicin into drug-sensitive K562 and drug-resistant leukemia K562/AO2 cells could be obviously observed by MTT assay and confocal fluorescence microscopy studies. These observations suggest that the new nanocomposites could readily induce daunorubicin to accumulate and uptake in target leukemia cells and increase the drug's cytotoxicity. Especially, the PLA/Au nanocomposites could significantly facilitate the cellular drug absorbtion of daunorubicin into drug-resistant K562/AO2 cells and efficiently inhibit the cancer cell proliferation. This raised the possibility to utilize the PLA/Au nanocomposites as a new effective additive agent to inhibit the drug resistance and thus as a novel strategy to sensitively track the respective cancer cells.

No MeSH data available.


Related in: MedlinePlus