Limits...
Facile Synthesis of Amine-Functionalized Eu 3+ -Doped La(OH) 3 Nanophosphors for Bioimaging

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Here, we report a straightforward synthesis process to produce colloidal Eu3+-activated nanophosphors (NPs) for use as bioimaging probes. In this procedure, poly(ethylene glycol) serves as a high-boiling point solvent allowing for nanoscale particle formation as well as a convenient medium for solvent exchange and subsequent surface modification. The La(OH)3:Eu3+ NPs produced by this process were ~3.5 nm in diameter as determined by transmission electron microscopy. The NP surface was coated with aminopropyltriethoxysilane to provide chemical functionality for attachment of biological ligands, improve chemical stability and prevent surface quenching of luminescent centers. Photoluminescence spectroscopy of the NPs displayed emission peaks at 597 and 615 nm (λex = 280 nm). The red emission, due to 5D0 → 7F1 and 5D0 → 7F2 transitions, was linear with concentration as observed by imaging with a conventional bioimaging system. To demonstrate the feasibility of these NPs to serve as optical probes in biological applications, an in vitro experiment was performed with HeLa cells. NP emission was observed in the cells by fluorescence microscopy. In addition, the NPs displayed no cytotoxicity over the course of a 48-h MTT cell viability assay. These results suggest that La(OH)3:Eu3+ NPs possess the potential to serve as a luminescent bioimaging probe.

No MeSH data available.


Nanophosphor emission intensity as a function of concentration. Image of optical phantoms containing 0, 0.01, 0.05, 0.1, 0.5 and 1.0 mg/mL NP, inset.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3211300&req=5

Figure 5: Nanophosphor emission intensity as a function of concentration. Image of optical phantoms containing 0, 0.01, 0.05, 0.1, 0.5 and 1.0 mg/mL NP, inset.

Mentions: In biological imaging applications where UV radiation can damage organic structures, blue excitation has been employed with Eu3+ complexes [37]. To demonstrate the use of these NPs in a conventional bioimaging system, optical phantoms containing various concentrations of APTES-coated NPs (0, 0.01, 0.05, 0.1, 0.5, 1.0 mg/mL) were analyzed in an IVIS Illumina fluorescence imaging system (λex = 430 nm). The fluorescence intensity, expressed in radiance, was measured and plotted as a function of concentration (Figure 5). The NPs displayed a linear relationship of luminescence with concentration potentially enabling their use in biological sensing and imaging applications.


Facile Synthesis of Amine-Functionalized Eu 3+ -Doped La(OH) 3 Nanophosphors for Bioimaging
Nanophosphor emission intensity as a function of concentration. Image of optical phantoms containing 0, 0.01, 0.05, 0.1, 0.5 and 1.0 mg/mL NP, inset.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211300&req=5

Figure 5: Nanophosphor emission intensity as a function of concentration. Image of optical phantoms containing 0, 0.01, 0.05, 0.1, 0.5 and 1.0 mg/mL NP, inset.
Mentions: In biological imaging applications where UV radiation can damage organic structures, blue excitation has been employed with Eu3+ complexes [37]. To demonstrate the use of these NPs in a conventional bioimaging system, optical phantoms containing various concentrations of APTES-coated NPs (0, 0.01, 0.05, 0.1, 0.5, 1.0 mg/mL) were analyzed in an IVIS Illumina fluorescence imaging system (λex = 430 nm). The fluorescence intensity, expressed in radiance, was measured and plotted as a function of concentration (Figure 5). The NPs displayed a linear relationship of luminescence with concentration potentially enabling their use in biological sensing and imaging applications.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Here, we report a straightforward synthesis process to produce colloidal Eu3+-activated nanophosphors (NPs) for use as bioimaging probes. In this procedure, poly(ethylene glycol) serves as a high-boiling point solvent allowing for nanoscale particle formation as well as a convenient medium for solvent exchange and subsequent surface modification. The La(OH)3:Eu3+ NPs produced by this process were ~3.5 nm in diameter as determined by transmission electron microscopy. The NP surface was coated with aminopropyltriethoxysilane to provide chemical functionality for attachment of biological ligands, improve chemical stability and prevent surface quenching of luminescent centers. Photoluminescence spectroscopy of the NPs displayed emission peaks at 597 and 615 nm (λex = 280 nm). The red emission, due to 5D0 → 7F1 and 5D0 → 7F2 transitions, was linear with concentration as observed by imaging with a conventional bioimaging system. To demonstrate the feasibility of these NPs to serve as optical probes in biological applications, an in vitro experiment was performed with HeLa cells. NP emission was observed in the cells by fluorescence microscopy. In addition, the NPs displayed no cytotoxicity over the course of a 48-h MTT cell viability assay. These results suggest that La(OH)3:Eu3+ NPs possess the potential to serve as a luminescent bioimaging probe.

No MeSH data available.