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Biomimetic Synthesis of Gelatin Polypeptide-Assisted Noble-Metal Nanoparticles and Their Interaction Study

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ABSTRACT

Herein, the generation of gold, silver, and silver–gold (Ag–Au) bimetallic nanoparticles was carried out in collagen (gelatin) solution. It first showed that the major ingredient in gelatin polypeptide, glutamic acid, acted as reducing agent to biomimetically synthesize noble metal nanoparticles at 80°C. The size of nanoparticles can be controlled not only by the mass ratio of gelatin to gold ion but also by pH of gelatin solution. Interaction between noble-metal nanoparticles and polypeptide has been investigated by TEM, UV–visible, fluorescence spectroscopy, and HNMR. This study testified that the degradation of gelatin protein could not alter the morphology of nanoparticles, but it made nanoparticles aggregated clusters array (opposing three-dimensional α-helix folding structure) into isolated nanoparticles stabilized by gelatin residues. This is a promising merit of gelatin to apply in the synthesis of nanoparticles. Therefore, gelatin protein is an excellent template for biomimetic synthesis of noble metal/bimetallic nanoparticle growth to form nanometer-sized device.

No MeSH data available.


a Fluorescence emission gelatin-AuNPs solutions (1–3, Excitation at 350 nm) and gelatin (4, Excitation at 335 nm) at the concentration of gelatin is 0.4 wt%, the concentrations of AuNPs are (1) 0.0006 M, (2) 0.0004 M, (3) 0.0002 M; b Stern–Volmer plot for gelatin-AuNPs.
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Figure 9: a Fluorescence emission gelatin-AuNPs solutions (1–3, Excitation at 350 nm) and gelatin (4, Excitation at 335 nm) at the concentration of gelatin is 0.4 wt%, the concentrations of AuNPs are (1) 0.0006 M, (2) 0.0004 M, (3) 0.0002 M; b Stern–Volmer plot for gelatin-AuNPs.

Mentions: The interaction between gelatin and gold nanoparticles has been investigated by Fluorescence techniques under pI. The potential interaction between gelatin and AuNPs is implied by the intrinsic fluorescence peaks at 395 nm that mainly originated from the lysine, tryptophan, and tyrosine residues. Figure 9a showed gold nanoparticles dramatically quenching the intrinsic fluorescence of gelatin protein. The fluorescence quenching mechanism can be analyzed quantitatively at 298 K with the Stern–Volmer equation [32].


Biomimetic Synthesis of Gelatin Polypeptide-Assisted Noble-Metal Nanoparticles and Their Interaction Study
a Fluorescence emission gelatin-AuNPs solutions (1–3, Excitation at 350 nm) and gelatin (4, Excitation at 335 nm) at the concentration of gelatin is 0.4 wt%, the concentrations of AuNPs are (1) 0.0006 M, (2) 0.0004 M, (3) 0.0002 M; b Stern–Volmer plot for gelatin-AuNPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211277&req=5

Figure 9: a Fluorescence emission gelatin-AuNPs solutions (1–3, Excitation at 350 nm) and gelatin (4, Excitation at 335 nm) at the concentration of gelatin is 0.4 wt%, the concentrations of AuNPs are (1) 0.0006 M, (2) 0.0004 M, (3) 0.0002 M; b Stern–Volmer plot for gelatin-AuNPs.
Mentions: The interaction between gelatin and gold nanoparticles has been investigated by Fluorescence techniques under pI. The potential interaction between gelatin and AuNPs is implied by the intrinsic fluorescence peaks at 395 nm that mainly originated from the lysine, tryptophan, and tyrosine residues. Figure 9a showed gold nanoparticles dramatically quenching the intrinsic fluorescence of gelatin protein. The fluorescence quenching mechanism can be analyzed quantitatively at 298 K with the Stern–Volmer equation [32].

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Herein, the generation of gold, silver, and silver–gold (Ag–Au) bimetallic nanoparticles was carried out in collagen (gelatin) solution. It first showed that the major ingredient in gelatin polypeptide, glutamic acid, acted as reducing agent to biomimetically synthesize noble metal nanoparticles at 80°C. The size of nanoparticles can be controlled not only by the mass ratio of gelatin to gold ion but also by pH of gelatin solution. Interaction between noble-metal nanoparticles and polypeptide has been investigated by TEM, UV–visible, fluorescence spectroscopy, and HNMR. This study testified that the degradation of gelatin protein could not alter the morphology of nanoparticles, but it made nanoparticles aggregated clusters array (opposing three-dimensional α-helix folding structure) into isolated nanoparticles stabilized by gelatin residues. This is a promising merit of gelatin to apply in the synthesis of nanoparticles. Therefore, gelatin protein is an excellent template for biomimetic synthesis of noble metal/bimetallic nanoparticle growth to form nanometer-sized device.

No MeSH data available.