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Promotion of allergic immune responses by intranasally-administrated nanosilica particles in mice.

Yoshida T, Yoshioka Y, Fujimura M, Yamashita K, Higashisaka K, Morishita Y, Kayamuro H, Nabeshi H, Nagano K, Abe Y, Kamada H, Tsunoda S, Itoh N, Yoshikawa T, Tsutsumi Y - Nanoscale Res Lett (2011)

Bottom Line: Intranasal exposure to OVA plus smaller nanosilica particles tended to induce a higher level of OVA-specific immunoglobulin (Ig) E, IgG and IgG1 Abs than did exposure to OVA plus larger silica particles.Taken together, these results indicate that nanosilica particles can induce allergen-specific Th2-type allergic immune responses in vivo.This study provides the foundations for the establishment of safe and effective forms of nanosilica particles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka 565-0871, Japan. yasuo@phs.osaka-u.ac.jp.

ABSTRACT
With the increase in use of nanomaterials, there is growing concern regarding their potential health risks. However, few studies have assessed the role of the different physical characteristics of nanomaterials in allergic responses. Here, we examined whether intranasally administered silica particles of various sizes have the capacity to promote allergic immune responses in mice. We used nanosilica particles with diameters of 30 or 70 nm (nSP30 or nSP70, respectively), and conventional micro-sized silica particles with diameters of 300 or 1000 nm (nSP300 or mSP1000, respectively). Mice were intranasally exposed to ovalbumin (OVA) plus each silica particle, and the levels of OVA-specific antibodies (Abs) in the plasma were determined. Intranasal exposure to OVA plus smaller nanosilica particles tended to induce a higher level of OVA-specific immunoglobulin (Ig) E, IgG and IgG1 Abs than did exposure to OVA plus larger silica particles. Splenocytes from mice exposed to OVA plus nSP30 secreted higher levels of Th2-type cytokines than mice exposed to OVA alone. Taken together, these results indicate that nanosilica particles can induce allergen-specific Th2-type allergic immune responses in vivo. This study provides the foundations for the establishment of safe and effective forms of nanosilica particles.

No MeSH data available.


Related in: MedlinePlus

Cytokine responses induced after intranasal exposure to OVA plus silica particles. BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. On day 21, splenocytes from each group were prepared and cultured with 1 mg/mL OVA. Culture supernatants were harvested after 3 days of incubation, and the level of OVA-induced IL-4 (A) and IL-5 (B) produced and released into the culture supernatant was analyzed using the Bio-Plex Multiplex Cytokine Assay. Data are presented as mean ± SEM (n = 3; *P < 0.05 vs OVA alone; †P < 0.05 vs PBS).
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Figure 3: Cytokine responses induced after intranasal exposure to OVA plus silica particles. BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. On day 21, splenocytes from each group were prepared and cultured with 1 mg/mL OVA. Culture supernatants were harvested after 3 days of incubation, and the level of OVA-induced IL-4 (A) and IL-5 (B) produced and released into the culture supernatant was analyzed using the Bio-Plex Multiplex Cytokine Assay. Data are presented as mean ± SEM (n = 3; *P < 0.05 vs OVA alone; †P < 0.05 vs PBS).

Mentions: To clarify the mechanism by which nSP30 elicited an immune response, we analyzed the profiles of cytokines released from splenocytes of OVA-exposed mice. The splenocytes were cultured in the presence of OVA in vitro, and the culture supernatants were assessed for Th2-type cytokines by using a multiplexed immunobeads assay. Splenocytes from mice exposed to OVA plus nSP30 exhibited higher levels of Th2-type cytokines (IL-4 and IL-5) than those induced with OVA alone (Figure 3). In contrast, there was hardly any difference in Th1-type cytokine (IFN-γ) production amongst all of the exposed mice (data not shown). In addition, nSP70, nSP300, and mSP1000 did not induce cytokine production (Figure 3). These results suggest that nSP30 nanosilica particle induces a Th2-type immune response in this experiment.


Promotion of allergic immune responses by intranasally-administrated nanosilica particles in mice.

Yoshida T, Yoshioka Y, Fujimura M, Yamashita K, Higashisaka K, Morishita Y, Kayamuro H, Nabeshi H, Nagano K, Abe Y, Kamada H, Tsunoda S, Itoh N, Yoshikawa T, Tsutsumi Y - Nanoscale Res Lett (2011)

Cytokine responses induced after intranasal exposure to OVA plus silica particles. BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. On day 21, splenocytes from each group were prepared and cultured with 1 mg/mL OVA. Culture supernatants were harvested after 3 days of incubation, and the level of OVA-induced IL-4 (A) and IL-5 (B) produced and released into the culture supernatant was analyzed using the Bio-Plex Multiplex Cytokine Assay. Data are presented as mean ± SEM (n = 3; *P < 0.05 vs OVA alone; †P < 0.05 vs PBS).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC3211251&req=5

Figure 3: Cytokine responses induced after intranasal exposure to OVA plus silica particles. BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. On day 21, splenocytes from each group were prepared and cultured with 1 mg/mL OVA. Culture supernatants were harvested after 3 days of incubation, and the level of OVA-induced IL-4 (A) and IL-5 (B) produced and released into the culture supernatant was analyzed using the Bio-Plex Multiplex Cytokine Assay. Data are presented as mean ± SEM (n = 3; *P < 0.05 vs OVA alone; †P < 0.05 vs PBS).
Mentions: To clarify the mechanism by which nSP30 elicited an immune response, we analyzed the profiles of cytokines released from splenocytes of OVA-exposed mice. The splenocytes were cultured in the presence of OVA in vitro, and the culture supernatants were assessed for Th2-type cytokines by using a multiplexed immunobeads assay. Splenocytes from mice exposed to OVA plus nSP30 exhibited higher levels of Th2-type cytokines (IL-4 and IL-5) than those induced with OVA alone (Figure 3). In contrast, there was hardly any difference in Th1-type cytokine (IFN-γ) production amongst all of the exposed mice (data not shown). In addition, nSP70, nSP300, and mSP1000 did not induce cytokine production (Figure 3). These results suggest that nSP30 nanosilica particle induces a Th2-type immune response in this experiment.

Bottom Line: Intranasal exposure to OVA plus smaller nanosilica particles tended to induce a higher level of OVA-specific immunoglobulin (Ig) E, IgG and IgG1 Abs than did exposure to OVA plus larger silica particles.Taken together, these results indicate that nanosilica particles can induce allergen-specific Th2-type allergic immune responses in vivo.This study provides the foundations for the establishment of safe and effective forms of nanosilica particles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka 565-0871, Japan. yasuo@phs.osaka-u.ac.jp.

ABSTRACT
With the increase in use of nanomaterials, there is growing concern regarding their potential health risks. However, few studies have assessed the role of the different physical characteristics of nanomaterials in allergic responses. Here, we examined whether intranasally administered silica particles of various sizes have the capacity to promote allergic immune responses in mice. We used nanosilica particles with diameters of 30 or 70 nm (nSP30 or nSP70, respectively), and conventional micro-sized silica particles with diameters of 300 or 1000 nm (nSP300 or mSP1000, respectively). Mice were intranasally exposed to ovalbumin (OVA) plus each silica particle, and the levels of OVA-specific antibodies (Abs) in the plasma were determined. Intranasal exposure to OVA plus smaller nanosilica particles tended to induce a higher level of OVA-specific immunoglobulin (Ig) E, IgG and IgG1 Abs than did exposure to OVA plus larger silica particles. Splenocytes from mice exposed to OVA plus nSP30 secreted higher levels of Th2-type cytokines than mice exposed to OVA alone. Taken together, these results indicate that nanosilica particles can induce allergen-specific Th2-type allergic immune responses in vivo. This study provides the foundations for the establishment of safe and effective forms of nanosilica particles.

No MeSH data available.


Related in: MedlinePlus