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Promotion of allergic immune responses by intranasally-administrated nanosilica particles in mice.

Yoshida T, Yoshioka Y, Fujimura M, Yamashita K, Higashisaka K, Morishita Y, Kayamuro H, Nabeshi H, Nagano K, Abe Y, Kamada H, Tsunoda S, Itoh N, Yoshikawa T, Tsutsumi Y - Nanoscale Res Lett (2011)

Bottom Line: Intranasal exposure to OVA plus smaller nanosilica particles tended to induce a higher level of OVA-specific immunoglobulin (Ig) E, IgG and IgG1 Abs than did exposure to OVA plus larger silica particles.Taken together, these results indicate that nanosilica particles can induce allergen-specific Th2-type allergic immune responses in vivo.This study provides the foundations for the establishment of safe and effective forms of nanosilica particles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka 565-0871, Japan. yasuo@phs.osaka-u.ac.jp.

ABSTRACT
With the increase in use of nanomaterials, there is growing concern regarding their potential health risks. However, few studies have assessed the role of the different physical characteristics of nanomaterials in allergic responses. Here, we examined whether intranasally administered silica particles of various sizes have the capacity to promote allergic immune responses in mice. We used nanosilica particles with diameters of 30 or 70 nm (nSP30 or nSP70, respectively), and conventional micro-sized silica particles with diameters of 300 or 1000 nm (nSP300 or mSP1000, respectively). Mice were intranasally exposed to ovalbumin (OVA) plus each silica particle, and the levels of OVA-specific antibodies (Abs) in the plasma were determined. Intranasal exposure to OVA plus smaller nanosilica particles tended to induce a higher level of OVA-specific immunoglobulin (Ig) E, IgG and IgG1 Abs than did exposure to OVA plus larger silica particles. Splenocytes from mice exposed to OVA plus nSP30 secreted higher levels of Th2-type cytokines than mice exposed to OVA alone. Taken together, these results indicate that nanosilica particles can induce allergen-specific Th2-type allergic immune responses in vivo. This study provides the foundations for the establishment of safe and effective forms of nanosilica particles.

No MeSH data available.


Related in: MedlinePlus

Plasma OVA-specific IgG and subclass IgG1 Ab response after intranasal exposure to OVA plus silica particles. BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. Plasma was collected on day 21 and analyzed by ELISA to detect the level of (a) OVA-specific IgG and (b) OVA-specific IgG1 Ab responses. Data represent mean absorbance at a wavelength of 450 nm (reference wavelength, 655 nm). N.D., not detected. Data are presented as mean ± SEM (n = 5 to 8); *P < 0.05, **P < 0.01 vs OVA alone; ††P < 0.01 vs PBS).
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Figure 2: Plasma OVA-specific IgG and subclass IgG1 Ab response after intranasal exposure to OVA plus silica particles. BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. Plasma was collected on day 21 and analyzed by ELISA to detect the level of (a) OVA-specific IgG and (b) OVA-specific IgG1 Ab responses. Data represent mean absorbance at a wavelength of 450 nm (reference wavelength, 655 nm). N.D., not detected. Data are presented as mean ± SEM (n = 5 to 8); *P < 0.05, **P < 0.01 vs OVA alone; ††P < 0.01 vs PBS).

Mentions: Next, to assess the types of immune responses elicited by silica particles, we measured the levels of anti-OVA IgG Ab and anti-OVA IgG1 Ab. IgG1 production is indicative of a Th2-type response. The levels of anti-OVA IgG and anti-OVA IgG1 Abs induced by intranasal-exposure to OVA plus smaller silica particles were higher than those induced by OVA plus larger silica particles (Figure 2); this was similar to the results observed for IgE Ab responses, described above (Figure 1). The levels of OVA-specific IgG Ab and OVA-specific IgG1 Ab in mice exposed to OVA plus nSP30 were significantly higher than in those exposed to OVA alone (Figure 2). These results suggest that nanosilica particles can induce the production of antigen-specific Ab responses including antigen-specific Th2 allergic immune responses.


Promotion of allergic immune responses by intranasally-administrated nanosilica particles in mice.

Yoshida T, Yoshioka Y, Fujimura M, Yamashita K, Higashisaka K, Morishita Y, Kayamuro H, Nabeshi H, Nagano K, Abe Y, Kamada H, Tsunoda S, Itoh N, Yoshikawa T, Tsutsumi Y - Nanoscale Res Lett (2011)

Plasma OVA-specific IgG and subclass IgG1 Ab response after intranasal exposure to OVA plus silica particles. BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. Plasma was collected on day 21 and analyzed by ELISA to detect the level of (a) OVA-specific IgG and (b) OVA-specific IgG1 Ab responses. Data represent mean absorbance at a wavelength of 450 nm (reference wavelength, 655 nm). N.D., not detected. Data are presented as mean ± SEM (n = 5 to 8); *P < 0.05, **P < 0.01 vs OVA alone; ††P < 0.01 vs PBS).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211251&req=5

Figure 2: Plasma OVA-specific IgG and subclass IgG1 Ab response after intranasal exposure to OVA plus silica particles. BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. Plasma was collected on day 21 and analyzed by ELISA to detect the level of (a) OVA-specific IgG and (b) OVA-specific IgG1 Ab responses. Data represent mean absorbance at a wavelength of 450 nm (reference wavelength, 655 nm). N.D., not detected. Data are presented as mean ± SEM (n = 5 to 8); *P < 0.05, **P < 0.01 vs OVA alone; ††P < 0.01 vs PBS).
Mentions: Next, to assess the types of immune responses elicited by silica particles, we measured the levels of anti-OVA IgG Ab and anti-OVA IgG1 Ab. IgG1 production is indicative of a Th2-type response. The levels of anti-OVA IgG and anti-OVA IgG1 Abs induced by intranasal-exposure to OVA plus smaller silica particles were higher than those induced by OVA plus larger silica particles (Figure 2); this was similar to the results observed for IgE Ab responses, described above (Figure 1). The levels of OVA-specific IgG Ab and OVA-specific IgG1 Ab in mice exposed to OVA plus nSP30 were significantly higher than in those exposed to OVA alone (Figure 2). These results suggest that nanosilica particles can induce the production of antigen-specific Ab responses including antigen-specific Th2 allergic immune responses.

Bottom Line: Intranasal exposure to OVA plus smaller nanosilica particles tended to induce a higher level of OVA-specific immunoglobulin (Ig) E, IgG and IgG1 Abs than did exposure to OVA plus larger silica particles.Taken together, these results indicate that nanosilica particles can induce allergen-specific Th2-type allergic immune responses in vivo.This study provides the foundations for the establishment of safe and effective forms of nanosilica particles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka 565-0871, Japan. yasuo@phs.osaka-u.ac.jp.

ABSTRACT
With the increase in use of nanomaterials, there is growing concern regarding their potential health risks. However, few studies have assessed the role of the different physical characteristics of nanomaterials in allergic responses. Here, we examined whether intranasally administered silica particles of various sizes have the capacity to promote allergic immune responses in mice. We used nanosilica particles with diameters of 30 or 70 nm (nSP30 or nSP70, respectively), and conventional micro-sized silica particles with diameters of 300 or 1000 nm (nSP300 or mSP1000, respectively). Mice were intranasally exposed to ovalbumin (OVA) plus each silica particle, and the levels of OVA-specific antibodies (Abs) in the plasma were determined. Intranasal exposure to OVA plus smaller nanosilica particles tended to induce a higher level of OVA-specific immunoglobulin (Ig) E, IgG and IgG1 Abs than did exposure to OVA plus larger silica particles. Splenocytes from mice exposed to OVA plus nSP30 secreted higher levels of Th2-type cytokines than mice exposed to OVA alone. Taken together, these results indicate that nanosilica particles can induce allergen-specific Th2-type allergic immune responses in vivo. This study provides the foundations for the establishment of safe and effective forms of nanosilica particles.

No MeSH data available.


Related in: MedlinePlus