A multi-dataset data-collection strategy produces better diffraction data.
Bottom Line: The theoretical analysis indicated that the MDS strategy can reduce the standard deviation (background noise) of diffraction data compared with the commonly used single-dataset strategy for a fixed X-ray dose.In order to validate the hypothesis experimentally, a data-quality evaluation process, termed a readiness test of the X-ray data-collection system, was developed.The data-collection results using home-laboratory-based rotating-anode X-ray and synchrotron X-ray systems indicate that the diffraction data collected with the MDS strategy contain more accurate anomalous signals from sulfur atoms than the data collected with a regular data-collection strategy.
Affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: The regular and MDS diffraction data from crystal 1 were selected to calculate the S-SAD-phased 2f o − f c electron-density map at 50.0–2.5 Å resolution as shown in Fig. 1 ▶. The map quality of the MDS data is clearly better than that of the regular-exposed data, which agrees with the Map cc values.
Affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. email@example.com