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Biomagnetic of Apatite-Coated Cobalt Ferrite: A Core – Shell Particle for Protein Adsorption and pH-Controlled Release

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ABSTRACT

Magnetic nanoparticle composite with a cobalt ferrite (CoFe2O4, (CF)) core and an apatite (Ap) coating was synthesized using a biomineralization process in which a modified simulated body fluid (1.5SBF) solution is the source of the calcium phosphate for the apatite formation. The core–shell structure formed after the citric acid–stabilized cobalt ferrite (CFCA) particles were incubated in the 1.5 SBF solution for 1 week. The mean particle size of CFCA-Ap is about 750 nm. A saturation magnetization of 15.56 emug-1 and a coercivity of 1808.5 Oe were observed for the CFCA-Ap obtained. Bovine serum albumin (BSA) was used as the model protein to study the adsorption and release of the proteins by the CFCA-Ap particles. The protein adsorption by the CFCA-Ap particles followed a more typical Freundlich than Langmuir adsorption isotherm. The BSA release as a function of time became less rapid as the CFCA-Ap particles were immersed in higher pH solution, thus indicating that the BSA release is dependent on the local pH.

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Release profiles of BSA protein adsorption on apatite (Ap)-coated citric acid–stabilized cobalt ferrite (CFCA) (CFCA-Ap) particles. The cumulative percent of BSA released versus incubation time for the BSA-loaded CFCA-Ap particles for three pH values (4.0, 6.5, and 7.4) of phosphate buffer saline. The data points are expressed as mean ± SD; n = 3.
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Figure 8: Release profiles of BSA protein adsorption on apatite (Ap)-coated citric acid–stabilized cobalt ferrite (CFCA) (CFCA-Ap) particles. The cumulative percent of BSA released versus incubation time for the BSA-loaded CFCA-Ap particles for three pH values (4.0, 6.5, and 7.4) of phosphate buffer saline. The data points are expressed as mean ± SD; n = 3.

Mentions: The concentration of the BSA that is released into PBS (at a pH = 4.0, 6.5 and 7.4) is obtained by comparing the measured absorbance of sample with absorbance on the calibration curve. The BSA release from CFCA-Ap composite particles is determined by plotting the cumulative data against time as illustrated in Figure 8. It reveals that the release rate of BSA from the CFCA-Ap is markedly influenced by the pH value. When the pH is 7.4 or 6.5, the initial release is seen to be lower in the first 5 h than when the pH is 4.0. A burst effect is seen when the pH is at this later value.


Biomagnetic of Apatite-Coated Cobalt Ferrite: A Core – Shell Particle for Protein Adsorption and pH-Controlled Release
Release profiles of BSA protein adsorption on apatite (Ap)-coated citric acid–stabilized cobalt ferrite (CFCA) (CFCA-Ap) particles. The cumulative percent of BSA released versus incubation time for the BSA-loaded CFCA-Ap particles for three pH values (4.0, 6.5, and 7.4) of phosphate buffer saline. The data points are expressed as mean ± SD; n = 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211243&req=5

Figure 8: Release profiles of BSA protein adsorption on apatite (Ap)-coated citric acid–stabilized cobalt ferrite (CFCA) (CFCA-Ap) particles. The cumulative percent of BSA released versus incubation time for the BSA-loaded CFCA-Ap particles for three pH values (4.0, 6.5, and 7.4) of phosphate buffer saline. The data points are expressed as mean ± SD; n = 3.
Mentions: The concentration of the BSA that is released into PBS (at a pH = 4.0, 6.5 and 7.4) is obtained by comparing the measured absorbance of sample with absorbance on the calibration curve. The BSA release from CFCA-Ap composite particles is determined by plotting the cumulative data against time as illustrated in Figure 8. It reveals that the release rate of BSA from the CFCA-Ap is markedly influenced by the pH value. When the pH is 7.4 or 6.5, the initial release is seen to be lower in the first 5 h than when the pH is 4.0. A burst effect is seen when the pH is at this later value.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Magnetic nanoparticle composite with a cobalt ferrite (CoFe2O4, (CF)) core and an apatite (Ap) coating was synthesized using a biomineralization process in which a modified simulated body fluid (1.5SBF) solution is the source of the calcium phosphate for the apatite formation. The core–shell structure formed after the citric acid–stabilized cobalt ferrite (CFCA) particles were incubated in the 1.5 SBF solution for 1 week. The mean particle size of CFCA-Ap is about 750 nm. A saturation magnetization of 15.56 emug-1 and a coercivity of 1808.5 Oe were observed for the CFCA-Ap obtained. Bovine serum albumin (BSA) was used as the model protein to study the adsorption and release of the proteins by the CFCA-Ap particles. The protein adsorption by the CFCA-Ap particles followed a more typical Freundlich than Langmuir adsorption isotherm. The BSA release as a function of time became less rapid as the CFCA-Ap particles were immersed in higher pH solution, thus indicating that the BSA release is dependent on the local pH.

No MeSH data available.


Related in: MedlinePlus