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Biomagnetic of Apatite-Coated Cobalt Ferrite: A Core – Shell Particle for Protein Adsorption and pH-Controlled Release

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ABSTRACT

Magnetic nanoparticle composite with a cobalt ferrite (CoFe2O4, (CF)) core and an apatite (Ap) coating was synthesized using a biomineralization process in which a modified simulated body fluid (1.5SBF) solution is the source of the calcium phosphate for the apatite formation. The core–shell structure formed after the citric acid–stabilized cobalt ferrite (CFCA) particles were incubated in the 1.5 SBF solution for 1 week. The mean particle size of CFCA-Ap is about 750 nm. A saturation magnetization of 15.56 emug-1 and a coercivity of 1808.5 Oe were observed for the CFCA-Ap obtained. Bovine serum albumin (BSA) was used as the model protein to study the adsorption and release of the proteins by the CFCA-Ap particles. The protein adsorption by the CFCA-Ap particles followed a more typical Freundlich than Langmuir adsorption isotherm. The BSA release as a function of time became less rapid as the CFCA-Ap particles were immersed in higher pH solution, thus indicating that the BSA release is dependent on the local pH.

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Flowchart for the apatite (Ap)-coated citric acid–stabilized cobalt ferrite (CFCA-Ap) synthesis via biomineralization from 1.5SBF solution method and BSA protein-loaded CFCA-Ap particles.
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Figure 1: Flowchart for the apatite (Ap)-coated citric acid–stabilized cobalt ferrite (CFCA-Ap) synthesis via biomineralization from 1.5SBF solution method and BSA protein-loaded CFCA-Ap particles.

Mentions: The procedure for the synthesis of citric acid-functionalized CoFe2O4 particles (CFCA) is shown in Figure 1. First, the CoFe2O4 particles are synthesized as outlined earlier. These CoFe2O4 particles are then dispersed in 0.001 M of citric acid (concentration 50 mg/ml) (UNiVAR, Australia) and stirred overnight. At the end of this stage, the samples consist of cobalt ferrite stabilized with carboxylic group (–COO-) and are labeled as CFCA. The suspension is separated by a permanent magnet and the resulting composite powder was freeze-dried. The citric acid–stabilized cobalt ferrite particles are coated by apatite following their immersion in the modified simulated body fluid (1.5SBF). The incubation was achieved by immersing the CFCA powders in 50 ml of 1.5SBF for 1 week at 37°C under static conditions. At this stage, the apatite layer was gradually formed on the CFCA surface. These particles are referred to as CFCA-Ap. After completing the immersion in the 1.5SBF, the particles were washed with de-ionized water and freeze-dried before any further analysis.


Biomagnetic of Apatite-Coated Cobalt Ferrite: A Core – Shell Particle for Protein Adsorption and pH-Controlled Release
Flowchart for the apatite (Ap)-coated citric acid–stabilized cobalt ferrite (CFCA-Ap) synthesis via biomineralization from 1.5SBF solution method and BSA protein-loaded CFCA-Ap particles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211243&req=5

Figure 1: Flowchart for the apatite (Ap)-coated citric acid–stabilized cobalt ferrite (CFCA-Ap) synthesis via biomineralization from 1.5SBF solution method and BSA protein-loaded CFCA-Ap particles.
Mentions: The procedure for the synthesis of citric acid-functionalized CoFe2O4 particles (CFCA) is shown in Figure 1. First, the CoFe2O4 particles are synthesized as outlined earlier. These CoFe2O4 particles are then dispersed in 0.001 M of citric acid (concentration 50 mg/ml) (UNiVAR, Australia) and stirred overnight. At the end of this stage, the samples consist of cobalt ferrite stabilized with carboxylic group (–COO-) and are labeled as CFCA. The suspension is separated by a permanent magnet and the resulting composite powder was freeze-dried. The citric acid–stabilized cobalt ferrite particles are coated by apatite following their immersion in the modified simulated body fluid (1.5SBF). The incubation was achieved by immersing the CFCA powders in 50 ml of 1.5SBF for 1 week at 37°C under static conditions. At this stage, the apatite layer was gradually formed on the CFCA surface. These particles are referred to as CFCA-Ap. After completing the immersion in the 1.5SBF, the particles were washed with de-ionized water and freeze-dried before any further analysis.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Magnetic nanoparticle composite with a cobalt ferrite (CoFe2O4, (CF)) core and an apatite (Ap) coating was synthesized using a biomineralization process in which a modified simulated body fluid (1.5SBF) solution is the source of the calcium phosphate for the apatite formation. The core–shell structure formed after the citric acid–stabilized cobalt ferrite (CFCA) particles were incubated in the 1.5 SBF solution for 1 week. The mean particle size of CFCA-Ap is about 750 nm. A saturation magnetization of 15.56 emug-1 and a coercivity of 1808.5 Oe were observed for the CFCA-Ap obtained. Bovine serum albumin (BSA) was used as the model protein to study the adsorption and release of the proteins by the CFCA-Ap particles. The protein adsorption by the CFCA-Ap particles followed a more typical Freundlich than Langmuir adsorption isotherm. The BSA release as a function of time became less rapid as the CFCA-Ap particles were immersed in higher pH solution, thus indicating that the BSA release is dependent on the local pH.

No MeSH data available.