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Silver and Gold Nanoparticles Alter Cathepsin Activity In vitro

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ABSTRACT

Nanomaterials are being incorporated into many biological applications for use as therapeutics, sensors, or labels. Silver nanomaterials are being utilized for biological implants and wound dressings as an antiviral material, whereas gold nanomaterials are being used as biological labels or sensors due to their surface properties and biocompatibility. Cytotoxicity data of these materials are becoming more prevalent; however, little research has been performed to understand how the introduction of these materials into cells affects cellular processes. Here, we demonstrate the impact that silver and gold nanoparticles have on cathepsin activity in vitro. Cathepsins are important cellular proteases that are imperative for proper immune system function. We have selected to examine gold and silver nanoparticles due to the increased use of these materials in biological applications. This manuscript depicts how both of these types of nanomaterials affect cathepsin activity, which could impact the host's immune system and its ability to respond to pathogens. Cathepsin B activity decreases in a dose-dependent manner with all nanoparticles tested. Alternatively, the impact of nanoparticles on cathepsin L activity depends greatly on the type and size of the material.

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Biocompatibility of Au-NPs in Vero cells. Cytotoxic levels were determined for 10- and 30-nm Au-NPs, following a 24-h exposure using a standard MTS cell viability assay. The cell viability in the treatment groups is expressed as percent control and plotted as the mean +/- SEM (n = 8).
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Figure 5: Biocompatibility of Au-NPs in Vero cells. Cytotoxic levels were determined for 10- and 30-nm Au-NPs, following a 24-h exposure using a standard MTS cell viability assay. The cell viability in the treatment groups is expressed as percent control and plotted as the mean +/- SEM (n = 8).

Mentions: The Au-NPs at 10 and 30 nm had minimal effects on the viability of Vero cells in culture (Figure 5). There was a slight decrease in the mitochondrial function of the Vero cells treated with very low (5 μg/ml) or very high (100 μg/ml) doses of Au-NP 10 (Figure 5), but a recovery was made by the cells following a 48-h exposure (data not shown).


Silver and Gold Nanoparticles Alter Cathepsin Activity In vitro
Biocompatibility of Au-NPs in Vero cells. Cytotoxic levels were determined for 10- and 30-nm Au-NPs, following a 24-h exposure using a standard MTS cell viability assay. The cell viability in the treatment groups is expressed as percent control and plotted as the mean +/- SEM (n = 8).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3211222&req=5

Figure 5: Biocompatibility of Au-NPs in Vero cells. Cytotoxic levels were determined for 10- and 30-nm Au-NPs, following a 24-h exposure using a standard MTS cell viability assay. The cell viability in the treatment groups is expressed as percent control and plotted as the mean +/- SEM (n = 8).
Mentions: The Au-NPs at 10 and 30 nm had minimal effects on the viability of Vero cells in culture (Figure 5). There was a slight decrease in the mitochondrial function of the Vero cells treated with very low (5 μg/ml) or very high (100 μg/ml) doses of Au-NP 10 (Figure 5), but a recovery was made by the cells following a 48-h exposure (data not shown).

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Nanomaterials are being incorporated into many biological applications for use as therapeutics, sensors, or labels. Silver nanomaterials are being utilized for biological implants and wound dressings as an antiviral material, whereas gold nanomaterials are being used as biological labels or sensors due to their surface properties and biocompatibility. Cytotoxicity data of these materials are becoming more prevalent; however, little research has been performed to understand how the introduction of these materials into cells affects cellular processes. Here, we demonstrate the impact that silver and gold nanoparticles have on cathepsin activity in vitro. Cathepsins are important cellular proteases that are imperative for proper immune system function. We have selected to examine gold and silver nanoparticles due to the increased use of these materials in biological applications. This manuscript depicts how both of these types of nanomaterials affect cathepsin activity, which could impact the host's immune system and its ability to respond to pathogens. Cathepsin B activity decreases in a dose-dependent manner with all nanoparticles tested. Alternatively, the impact of nanoparticles on cathepsin L activity depends greatly on the type and size of the material.

No MeSH data available.


Related in: MedlinePlus