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Mouse mammary tumor virus-like gene sequences are present in lung patient specimens.

Trejo-Avila LM, Zapata-Benavides P, Barrera-Rodríguez R, Badillo-Almaráz I, Saavedra-Alonso S, Zamora-Avila DE, Morán-Santibañez K, Garza-Sáenz JA, Tamez-Guerra R, Rodríguez-Padilla C - Virol. J. (2011)

Bottom Line: The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347.The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample.Two of the env sequences exhibited disruption of the reading frame due to mutations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Ave, Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, 66451, Mexico. laura.trejoav@uanl.edu.mx

ABSTRACT

Background: Previous studies have reported on the presence of Murine Mammary Tumor Virus (MMTV)-like gene sequences in human cancer tissue specimens. Here, we search for MMTV-like gene sequences in lung diseases including carcinomas specimens from a Mexican population. This study was based on our previous study reporting that the INER51 lung cancer cell line, from a pleural effusion of a Mexican patient, contains MMTV-like env gene sequences.

Results: The MMTV-like env gene sequences have been detected in three out of 18 specimens studied, by PCR using a specific set of MMTV-like primers. The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347. The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample. Two of the env sequences exhibited disruption of the reading frame due to mutations.

Conclusion: In summary, we identified the presence of MMTV-like gene sequences in 2 out of 11 (18%) of the lung carcinomas and 1 out of 7 (14%) of acute inflamatory lung infiltrate specimens studied of a Mexican Population.

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MMTV-like env gene sequencing. Comparison of env gene sequences amplified from INER6 (GU252129), HZ-101 (HM636471), HZ-14 (HM636470), and INER51 (DQ367729).
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Figure 4: MMTV-like env gene sequencing. Comparison of env gene sequences amplified from INER6 (GU252129), HZ-101 (HM636471), HZ-14 (HM636470), and INER51 (DQ367729).

Mentions: The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute lung inflammation infiltrate specimen sample. The homology of the INER6, HZ-101, and HZ-14 sequences of the MMTV env region were 99%, 98%, and 97% respectively, as compared to Gen Bank accession number AY152722. The INER6 and HZ-101 sequences were mutated in their reading frame, resulting in nonsense mutations (Figure 4).


Mouse mammary tumor virus-like gene sequences are present in lung patient specimens.

Trejo-Avila LM, Zapata-Benavides P, Barrera-Rodríguez R, Badillo-Almaráz I, Saavedra-Alonso S, Zamora-Avila DE, Morán-Santibañez K, Garza-Sáenz JA, Tamez-Guerra R, Rodríguez-Padilla C - Virol. J. (2011)

MMTV-like env gene sequencing. Comparison of env gene sequences amplified from INER6 (GU252129), HZ-101 (HM636471), HZ-14 (HM636470), and INER51 (DQ367729).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198956&req=5

Figure 4: MMTV-like env gene sequencing. Comparison of env gene sequences amplified from INER6 (GU252129), HZ-101 (HM636471), HZ-14 (HM636470), and INER51 (DQ367729).
Mentions: The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute lung inflammation infiltrate specimen sample. The homology of the INER6, HZ-101, and HZ-14 sequences of the MMTV env region were 99%, 98%, and 97% respectively, as compared to Gen Bank accession number AY152722. The INER6 and HZ-101 sequences were mutated in their reading frame, resulting in nonsense mutations (Figure 4).

Bottom Line: The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347.The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample.Two of the env sequences exhibited disruption of the reading frame due to mutations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Ave, Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, 66451, Mexico. laura.trejoav@uanl.edu.mx

ABSTRACT

Background: Previous studies have reported on the presence of Murine Mammary Tumor Virus (MMTV)-like gene sequences in human cancer tissue specimens. Here, we search for MMTV-like gene sequences in lung diseases including carcinomas specimens from a Mexican population. This study was based on our previous study reporting that the INER51 lung cancer cell line, from a pleural effusion of a Mexican patient, contains MMTV-like env gene sequences.

Results: The MMTV-like env gene sequences have been detected in three out of 18 specimens studied, by PCR using a specific set of MMTV-like primers. The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347. The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample. Two of the env sequences exhibited disruption of the reading frame due to mutations.

Conclusion: In summary, we identified the presence of MMTV-like gene sequences in 2 out of 11 (18%) of the lung carcinomas and 1 out of 7 (14%) of acute inflamatory lung infiltrate specimens studied of a Mexican Population.

Show MeSH
Related in: MedlinePlus