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Mouse mammary tumor virus-like gene sequences are present in lung patient specimens.

Trejo-Avila LM, Zapata-Benavides P, Barrera-Rodríguez R, Badillo-Almaráz I, Saavedra-Alonso S, Zamora-Avila DE, Morán-Santibañez K, Garza-Sáenz JA, Tamez-Guerra R, Rodríguez-Padilla C - Virol. J. (2011)

Bottom Line: The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347.The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample.Two of the env sequences exhibited disruption of the reading frame due to mutations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Ave, Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, 66451, Mexico. laura.trejoav@uanl.edu.mx

ABSTRACT

Background: Previous studies have reported on the presence of Murine Mammary Tumor Virus (MMTV)-like gene sequences in human cancer tissue specimens. Here, we search for MMTV-like gene sequences in lung diseases including carcinomas specimens from a Mexican population. This study was based on our previous study reporting that the INER51 lung cancer cell line, from a pleural effusion of a Mexican patient, contains MMTV-like env gene sequences.

Results: The MMTV-like env gene sequences have been detected in three out of 18 specimens studied, by PCR using a specific set of MMTV-like primers. The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347. The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample. Two of the env sequences exhibited disruption of the reading frame due to mutations.

Conclusion: In summary, we identified the presence of MMTV-like gene sequences in 2 out of 11 (18%) of the lung carcinomas and 1 out of 7 (14%) of acute inflamatory lung infiltrate specimens studied of a Mexican Population.

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Related in: MedlinePlus

Amplification of MMTV-like env gene sequences in lung samples. Agarose gel electrophoresis of the 665-bp PCR product using primers 1-3 (top panel). The 595-bp PCR product using the 5L-3L primers (middle panel). As a PCR control, we used G3PDH primers to amplify a 452-bp product (bottom panel). Lane M contains a marker; lane 1 is INER51; lane 2 is INER6; lane 3 is IN-9; lane 4 is HZ-14; lane 5 is HZ-19; and lane 6 is HZ-101.
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Figure 2: Amplification of MMTV-like env gene sequences in lung samples. Agarose gel electrophoresis of the 665-bp PCR product using primers 1-3 (top panel). The 595-bp PCR product using the 5L-3L primers (middle panel). As a PCR control, we used G3PDH primers to amplify a 452-bp product (bottom panel). Lane M contains a marker; lane 1 is INER51; lane 2 is INER6; lane 3 is IN-9; lane 4 is HZ-14; lane 5 is HZ-19; and lane 6 is HZ-101.

Mentions: We analyzed 11 lung cancer samples and seven specimens with other lung pathologies (Table 1). The lung samples were amplified with primers 1-3, and nested PCR was performed using primers 5L-3L. In both PCR tests, the sequences were amplified in three samples, as shown in figure 2. To confirm the presence of the MMTV-like gene sequences.in the lung samples, the three positive samples were amplified using primers of a LTR-gag region as shown in figure 3.


Mouse mammary tumor virus-like gene sequences are present in lung patient specimens.

Trejo-Avila LM, Zapata-Benavides P, Barrera-Rodríguez R, Badillo-Almaráz I, Saavedra-Alonso S, Zamora-Avila DE, Morán-Santibañez K, Garza-Sáenz JA, Tamez-Guerra R, Rodríguez-Padilla C - Virol. J. (2011)

Amplification of MMTV-like env gene sequences in lung samples. Agarose gel electrophoresis of the 665-bp PCR product using primers 1-3 (top panel). The 595-bp PCR product using the 5L-3L primers (middle panel). As a PCR control, we used G3PDH primers to amplify a 452-bp product (bottom panel). Lane M contains a marker; lane 1 is INER51; lane 2 is INER6; lane 3 is IN-9; lane 4 is HZ-14; lane 5 is HZ-19; and lane 6 is HZ-101.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198956&req=5

Figure 2: Amplification of MMTV-like env gene sequences in lung samples. Agarose gel electrophoresis of the 665-bp PCR product using primers 1-3 (top panel). The 595-bp PCR product using the 5L-3L primers (middle panel). As a PCR control, we used G3PDH primers to amplify a 452-bp product (bottom panel). Lane M contains a marker; lane 1 is INER51; lane 2 is INER6; lane 3 is IN-9; lane 4 is HZ-14; lane 5 is HZ-19; and lane 6 is HZ-101.
Mentions: We analyzed 11 lung cancer samples and seven specimens with other lung pathologies (Table 1). The lung samples were amplified with primers 1-3, and nested PCR was performed using primers 5L-3L. In both PCR tests, the sequences were amplified in three samples, as shown in figure 2. To confirm the presence of the MMTV-like gene sequences.in the lung samples, the three positive samples were amplified using primers of a LTR-gag region as shown in figure 3.

Bottom Line: The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347.The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample.Two of the env sequences exhibited disruption of the reading frame due to mutations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Ave, Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, 66451, Mexico. laura.trejoav@uanl.edu.mx

ABSTRACT

Background: Previous studies have reported on the presence of Murine Mammary Tumor Virus (MMTV)-like gene sequences in human cancer tissue specimens. Here, we search for MMTV-like gene sequences in lung diseases including carcinomas specimens from a Mexican population. This study was based on our previous study reporting that the INER51 lung cancer cell line, from a pleural effusion of a Mexican patient, contains MMTV-like env gene sequences.

Results: The MMTV-like env gene sequences have been detected in three out of 18 specimens studied, by PCR using a specific set of MMTV-like primers. The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347. The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample. Two of the env sequences exhibited disruption of the reading frame due to mutations.

Conclusion: In summary, we identified the presence of MMTV-like gene sequences in 2 out of 11 (18%) of the lung carcinomas and 1 out of 7 (14%) of acute inflamatory lung infiltrate specimens studied of a Mexican Population.

Show MeSH
Related in: MedlinePlus