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Aging-associated increase in indoleamine 2,3-dioxygenase (IDO) activity appears to be unrelated to the transcription of the IDO1 or IDO2 genes in peripheral blood mononuclear cells.

Marttila S, Jylhävä J, Eklund C, Hervonen A, Jylhä M, Hurme M - Immun Ageing (2011)

Bottom Line: Elevated levels of pro-inflammatory cytokines have been associated with several age-associated diseases and with a shortened lifespan.We have previously shown that IDO activity is increased in nonagenarians compared to young individuals and that high IDO activity is associated with mortality at old age.Our results show that IDO1 and IDO2 are not differently expressed in nonagenarians compared to controls and that the expression of IDO genes is not associated with the level of IDO activity in the plasma.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Immunology, The School of Medicine, University of Tampere, Finland. saara.marttila@uta.fi.

ABSTRACT

Background: Old age is associated with increased levels of circulating pro-inflammatory cytokines, a phenomenon termed inflamm-aging. Elevated levels of pro-inflammatory cytokines have been associated with several age-associated diseases and with a shortened lifespan. Indoleamine 2,3-dioxygenase (IDO) has immunomodulatory properties and its activity is elevated in inflammation, autoimmune disorders and malignancies. We have previously shown that IDO activity is increased in nonagenarians compared to young individuals and that high IDO activity is associated with mortality at old age.

Findings: In this study our aim was to assess whether this difference in IDO activity in the plasma was due to the differential expression of either the IDO1 or IDO2 gene in peripheral blood mononuclear cells. Our results show that IDO1 and IDO2 are not differently expressed in nonagenarians compared to controls and that the expression of IDO genes is not associated with the level of IDO activity in the plasma.

Conclusion: The level of IDO activity in the plasma is not regulated through the expression of IDO1 or IDO2 in the peripheral blood mononuclear cells.

No MeSH data available.


Related in: MedlinePlus

IDO activity and expression. The level of IDO enzyme activity in the plasma does not correlate with the level of IDO1 or IDO2 expression in PBMCs in the whole population (Spearman's rho -0.503, p = 0.095; rho = 0.077 p = 0.812, respectively), nor in the nonagenarians or young controls separately.
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Figure 2: IDO activity and expression. The level of IDO enzyme activity in the plasma does not correlate with the level of IDO1 or IDO2 expression in PBMCs in the whole population (Spearman's rho -0.503, p = 0.095; rho = 0.077 p = 0.812, respectively), nor in the nonagenarians or young controls separately.

Mentions: As in our previous work [14], there was a significant difference in the IDO activity in the plasma between the nonagenarians and the young controls; the level of IDO activity is higher in the plasma of the nonagenarians compared to young controls (Figure 1). However, the data obtained showed that there was no difference in the expression levels of IDO1 and IDO2 in nonagenarians compared to young controls (Table 1). Neither did the level of IDO1 or IDO2 expression in PBMCs correlate with the IDO activity in the plasma in nonagenarians or in young controls (Figure 2).


Aging-associated increase in indoleamine 2,3-dioxygenase (IDO) activity appears to be unrelated to the transcription of the IDO1 or IDO2 genes in peripheral blood mononuclear cells.

Marttila S, Jylhävä J, Eklund C, Hervonen A, Jylhä M, Hurme M - Immun Ageing (2011)

IDO activity and expression. The level of IDO enzyme activity in the plasma does not correlate with the level of IDO1 or IDO2 expression in PBMCs in the whole population (Spearman's rho -0.503, p = 0.095; rho = 0.077 p = 0.812, respectively), nor in the nonagenarians or young controls separately.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198939&req=5

Figure 2: IDO activity and expression. The level of IDO enzyme activity in the plasma does not correlate with the level of IDO1 or IDO2 expression in PBMCs in the whole population (Spearman's rho -0.503, p = 0.095; rho = 0.077 p = 0.812, respectively), nor in the nonagenarians or young controls separately.
Mentions: As in our previous work [14], there was a significant difference in the IDO activity in the plasma between the nonagenarians and the young controls; the level of IDO activity is higher in the plasma of the nonagenarians compared to young controls (Figure 1). However, the data obtained showed that there was no difference in the expression levels of IDO1 and IDO2 in nonagenarians compared to young controls (Table 1). Neither did the level of IDO1 or IDO2 expression in PBMCs correlate with the IDO activity in the plasma in nonagenarians or in young controls (Figure 2).

Bottom Line: Elevated levels of pro-inflammatory cytokines have been associated with several age-associated diseases and with a shortened lifespan.We have previously shown that IDO activity is increased in nonagenarians compared to young individuals and that high IDO activity is associated with mortality at old age.Our results show that IDO1 and IDO2 are not differently expressed in nonagenarians compared to controls and that the expression of IDO genes is not associated with the level of IDO activity in the plasma.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Immunology, The School of Medicine, University of Tampere, Finland. saara.marttila@uta.fi.

ABSTRACT

Background: Old age is associated with increased levels of circulating pro-inflammatory cytokines, a phenomenon termed inflamm-aging. Elevated levels of pro-inflammatory cytokines have been associated with several age-associated diseases and with a shortened lifespan. Indoleamine 2,3-dioxygenase (IDO) has immunomodulatory properties and its activity is elevated in inflammation, autoimmune disorders and malignancies. We have previously shown that IDO activity is increased in nonagenarians compared to young individuals and that high IDO activity is associated with mortality at old age.

Findings: In this study our aim was to assess whether this difference in IDO activity in the plasma was due to the differential expression of either the IDO1 or IDO2 gene in peripheral blood mononuclear cells. Our results show that IDO1 and IDO2 are not differently expressed in nonagenarians compared to controls and that the expression of IDO genes is not associated with the level of IDO activity in the plasma.

Conclusion: The level of IDO activity in the plasma is not regulated through the expression of IDO1 or IDO2 in the peripheral blood mononuclear cells.

No MeSH data available.


Related in: MedlinePlus