Limits...
Magnesium deficiency induces anxiety and HPA axis dysregulation: modulation by therapeutic drug treatment.

Sartori SB, Whittle N, Hetzenauer A, Singewald N - Neuropharmacology (2011)

Bottom Line: Since there is evidence that Mg(2+) modulates the hypothalamic-pituitary adrenal (HPA) axis, we tested whether enhanced anxiety-like behaviour can be reliably elicited by dietary Mg(2+) deficiency and whether Mg(2+) deficiency is associated with altered HPA axis function.It is further suggested that dysregulations in the HPA axis may contribute to the hyper-emotionality in response to dietary induced hypomagnesaemia.This article is part of a Special Issue entitled 'Anxiety and Depression'.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Institute of Pharmacy, and Centre for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria. simone.sartori@uibk.ac.at

Show MeSH

Related in: MedlinePlus

This figure shows (A) representative autoradiograms and (B) quantification of prepro-CRH mRNA expression in the paraventricular hypothalamic nucleus for C57Bl/6N mice fed either the control diet (control; n = 10), the Mg2+ deficient diet (MgD; n = 10), or the Mg2+ deficient diet after long-term treatment with desipramine (MgD + DMI; n = 10); it also provides quantification of unspecific hybridisation. Data represent mean ± SEM. *P < 0.05, ***P < 0.001 for Mg2+ deficient vs. control mice and ###P < 0.001 for desipramine-treated Mg2+ deficient mice vs. Mg2+ deficient mice.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3198864&req=5

fig3: This figure shows (A) representative autoradiograms and (B) quantification of prepro-CRH mRNA expression in the paraventricular hypothalamic nucleus for C57Bl/6N mice fed either the control diet (control; n = 10), the Mg2+ deficient diet (MgD; n = 10), or the Mg2+ deficient diet after long-term treatment with desipramine (MgD + DMI; n = 10); it also provides quantification of unspecific hybridisation. Data represent mean ± SEM. *P < 0.05, ***P < 0.001 for Mg2+ deficient vs. control mice and ###P < 0.001 for desipramine-treated Mg2+ deficient mice vs. Mg2+ deficient mice.

Mentions: In C57Bl/6N mice prepro-CRH mRNA expression, as visualised by in situ hybridisation, was high in the PVN and moderate in the central amygdala and Barrington’s nucleus (Table 2), which is in good accordance with previous studies (e.g. Keegan et al., 1994). In the PVN a significant group effect was observed (F2,29 = 12.486, P < 0.001) as the abundance of prepro-CRH mRNA was increased in Mg2+ deficient mice compared with mice fed the control diet (P < 0.001), and chronic treatment with desipramine normalised this effect in Mg2+ deficient mice (P < 0.001) (Table 2, Fig. 3). In contrast to the PVN, prepro-CRH mRNA expression did not differ between experimental groups in the central amygdala (n.s.) or in the Barrington’s nucleus (n.s.) (Table 2).


Magnesium deficiency induces anxiety and HPA axis dysregulation: modulation by therapeutic drug treatment.

Sartori SB, Whittle N, Hetzenauer A, Singewald N - Neuropharmacology (2011)

This figure shows (A) representative autoradiograms and (B) quantification of prepro-CRH mRNA expression in the paraventricular hypothalamic nucleus for C57Bl/6N mice fed either the control diet (control; n = 10), the Mg2+ deficient diet (MgD; n = 10), or the Mg2+ deficient diet after long-term treatment with desipramine (MgD + DMI; n = 10); it also provides quantification of unspecific hybridisation. Data represent mean ± SEM. *P < 0.05, ***P < 0.001 for Mg2+ deficient vs. control mice and ###P < 0.001 for desipramine-treated Mg2+ deficient mice vs. Mg2+ deficient mice.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198864&req=5

fig3: This figure shows (A) representative autoradiograms and (B) quantification of prepro-CRH mRNA expression in the paraventricular hypothalamic nucleus for C57Bl/6N mice fed either the control diet (control; n = 10), the Mg2+ deficient diet (MgD; n = 10), or the Mg2+ deficient diet after long-term treatment with desipramine (MgD + DMI; n = 10); it also provides quantification of unspecific hybridisation. Data represent mean ± SEM. *P < 0.05, ***P < 0.001 for Mg2+ deficient vs. control mice and ###P < 0.001 for desipramine-treated Mg2+ deficient mice vs. Mg2+ deficient mice.
Mentions: In C57Bl/6N mice prepro-CRH mRNA expression, as visualised by in situ hybridisation, was high in the PVN and moderate in the central amygdala and Barrington’s nucleus (Table 2), which is in good accordance with previous studies (e.g. Keegan et al., 1994). In the PVN a significant group effect was observed (F2,29 = 12.486, P < 0.001) as the abundance of prepro-CRH mRNA was increased in Mg2+ deficient mice compared with mice fed the control diet (P < 0.001), and chronic treatment with desipramine normalised this effect in Mg2+ deficient mice (P < 0.001) (Table 2, Fig. 3). In contrast to the PVN, prepro-CRH mRNA expression did not differ between experimental groups in the central amygdala (n.s.) or in the Barrington’s nucleus (n.s.) (Table 2).

Bottom Line: Since there is evidence that Mg(2+) modulates the hypothalamic-pituitary adrenal (HPA) axis, we tested whether enhanced anxiety-like behaviour can be reliably elicited by dietary Mg(2+) deficiency and whether Mg(2+) deficiency is associated with altered HPA axis function.It is further suggested that dysregulations in the HPA axis may contribute to the hyper-emotionality in response to dietary induced hypomagnesaemia.This article is part of a Special Issue entitled 'Anxiety and Depression'.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Institute of Pharmacy, and Centre for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria. simone.sartori@uibk.ac.at

Show MeSH
Related in: MedlinePlus