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Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

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The levels of cell phenotype in blood following a single instillation with DEPs (n = 4).Whole blood was harvested from 16 mice on day 1, 7, 14, and 28 after DEP instillation, and pooled to make 4 samples for cell phenotype analysis. (A) control, (B) day 1, (C) day 7, (D) day 14.
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pone-0026749-g008: The levels of cell phenotype in blood following a single instillation with DEPs (n = 4).Whole blood was harvested from 16 mice on day 1, 7, 14, and 28 after DEP instillation, and pooled to make 4 samples for cell phenotype analysis. (A) control, (B) day 1, (C) day 7, (D) day 14.

Mentions: As shown in Table 3 and Figure 8, the cell distribution of lymphocytes shifted to a T-cell-dominant direction with the increase in CD8+ T cells on day 1, but shifted to a B-cell-dominant direction with the recovery of the ratio of CD4+/CD8+ on day 7 after treatment. On day 14, the ratio of CD4+/CD8+ cells were clearly increased, implying an increase in the number of CD4+ T cells; however, the distribution of T and B cells did not change significantly. Furthermore, a significant increase in the number of NK cells was shown during the experimental period.


Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

The levels of cell phenotype in blood following a single instillation with DEPs (n = 4).Whole blood was harvested from 16 mice on day 1, 7, 14, and 28 after DEP instillation, and pooled to make 4 samples for cell phenotype analysis. (A) control, (B) day 1, (C) day 7, (D) day 14.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198839&req=5

pone-0026749-g008: The levels of cell phenotype in blood following a single instillation with DEPs (n = 4).Whole blood was harvested from 16 mice on day 1, 7, 14, and 28 after DEP instillation, and pooled to make 4 samples for cell phenotype analysis. (A) control, (B) day 1, (C) day 7, (D) day 14.
Mentions: As shown in Table 3 and Figure 8, the cell distribution of lymphocytes shifted to a T-cell-dominant direction with the increase in CD8+ T cells on day 1, but shifted to a B-cell-dominant direction with the recovery of the ratio of CD4+/CD8+ on day 7 after treatment. On day 14, the ratio of CD4+/CD8+ cells were clearly increased, implying an increase in the number of CD4+ T cells; however, the distribution of T and B cells did not change significantly. Furthermore, a significant increase in the number of NK cells was shown during the experimental period.

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

Show MeSH
Related in: MedlinePlus