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Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

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Levels of histamine in BAL fluid and in blood following a single instillation with DEPs (n = 4).BAL fluid and serum were harvested and pooled on day 1, 7, 14, and 28 after DEP instillation. The level in each group was expressed as the mean ± SD of the values measured. *P<0.05; **P<0.01.
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pone-0026749-g007: Levels of histamine in BAL fluid and in blood following a single instillation with DEPs (n = 4).BAL fluid and serum were harvested and pooled on day 1, 7, 14, and 28 after DEP instillation. The level in each group was expressed as the mean ± SD of the values measured. *P<0.05; **P<0.01.

Mentions: The post-treatment levels of histamine in the BAL fluid and blood were significantly greater than that in the controls (Fig. 7). The level of histamine in the BAL fluid was almost the same as that in the control throughout the experimental period (1.2- to 1.3-fold that in the control). The blood levels of histamine were 13.7±0.5, 11.9±0.8, 7.2±0.1, and 7.8±0.2 pg/ml on days 1, 7, 14, and 28 after treatment, while the level in the controls was 5.1±0.02 pg/ml.


Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Levels of histamine in BAL fluid and in blood following a single instillation with DEPs (n = 4).BAL fluid and serum were harvested and pooled on day 1, 7, 14, and 28 after DEP instillation. The level in each group was expressed as the mean ± SD of the values measured. *P<0.05; **P<0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198839&req=5

pone-0026749-g007: Levels of histamine in BAL fluid and in blood following a single instillation with DEPs (n = 4).BAL fluid and serum were harvested and pooled on day 1, 7, 14, and 28 after DEP instillation. The level in each group was expressed as the mean ± SD of the values measured. *P<0.05; **P<0.01.
Mentions: The post-treatment levels of histamine in the BAL fluid and blood were significantly greater than that in the controls (Fig. 7). The level of histamine in the BAL fluid was almost the same as that in the control throughout the experimental period (1.2- to 1.3-fold that in the control). The blood levels of histamine were 13.7±0.5, 11.9±0.8, 7.2±0.1, and 7.8±0.2 pg/ml on days 1, 7, 14, and 28 after treatment, while the level in the controls was 5.1±0.02 pg/ml.

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

Show MeSH
Related in: MedlinePlus