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Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

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Related in: MedlinePlus

Levels of TGF-β in BAL fluid and blood after a single instillation of DEPs (n = 4).BAL fluid and serum samples were harvested and pooled on days 1, 7, 14, and 28 after DEP instillation, and pooled (500 µL per mouse) to 4 test samples per group for further analysis, respectively. The levels in each group were calculated as mean ± SD of the values measured. *P<0.05; **P<0.01.
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pone-0026749-g006: Levels of TGF-β in BAL fluid and blood after a single instillation of DEPs (n = 4).BAL fluid and serum samples were harvested and pooled on days 1, 7, 14, and 28 after DEP instillation, and pooled (500 µL per mouse) to 4 test samples per group for further analysis, respectively. The levels in each group were calculated as mean ± SD of the values measured. *P<0.05; **P<0.01.

Mentions: TGF-β levels in blood were significantly greater than that in the controls at all time points, whereas the levels of TGF-β in BAL fluid were significantly greater than those in the controls only on day 28 after treatment (Fig. 6). Blood levels of TGF-β were 9.35±0.14, 3.05±0.05, 5.08±0.06, and 23.81±0.76 pg/ml on days 1, 7, 14, and 28 after treatment, respectively, while that in the controls was 1.12±0.00 pg/ml.


Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Levels of TGF-β in BAL fluid and blood after a single instillation of DEPs (n = 4).BAL fluid and serum samples were harvested and pooled on days 1, 7, 14, and 28 after DEP instillation, and pooled (500 µL per mouse) to 4 test samples per group for further analysis, respectively. The levels in each group were calculated as mean ± SD of the values measured. *P<0.05; **P<0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198839&req=5

pone-0026749-g006: Levels of TGF-β in BAL fluid and blood after a single instillation of DEPs (n = 4).BAL fluid and serum samples were harvested and pooled on days 1, 7, 14, and 28 after DEP instillation, and pooled (500 µL per mouse) to 4 test samples per group for further analysis, respectively. The levels in each group were calculated as mean ± SD of the values measured. *P<0.05; **P<0.01.
Mentions: TGF-β levels in blood were significantly greater than that in the controls at all time points, whereas the levels of TGF-β in BAL fluid were significantly greater than those in the controls only on day 28 after treatment (Fig. 6). Blood levels of TGF-β were 9.35±0.14, 3.05±0.05, 5.08±0.06, and 23.81±0.76 pg/ml on days 1, 7, 14, and 28 after treatment, respectively, while that in the controls was 1.12±0.00 pg/ml.

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

Show MeSH
Related in: MedlinePlus