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Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

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Related in: MedlinePlus

Changes in the protein expression in lung tissue after a single instillation of DEPs.Results were confirmed by several separate experiments, and representative images are shown.
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pone-0026749-g005: Changes in the protein expression in lung tissue after a single instillation of DEPs.Results were confirmed by several separate experiments, and representative images are shown.

Mentions: Protein expression of RANTES, p53, and phospho-p53 in lung tissue increased in a time-dependent manner and that of TGF-β was clearly induced only on day 28 after treatment (Fig. 5). The concentration of inducible nitric oxide synthatase (iNOS) increased significantly during the experimental period and peaked on day 7 after treatment, whereas expression of COX-2 was upregulated from day 1 to day 14 after treatment. The effect of DEP exposure on the expression of mesothelin protein was minimal. The protein expression levels of p-IκBα, p-STAT3, and NFκB p65 peaked on day 14 after treatment.


Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Changes in the protein expression in lung tissue after a single instillation of DEPs.Results were confirmed by several separate experiments, and representative images are shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198839&req=5

pone-0026749-g005: Changes in the protein expression in lung tissue after a single instillation of DEPs.Results were confirmed by several separate experiments, and representative images are shown.
Mentions: Protein expression of RANTES, p53, and phospho-p53 in lung tissue increased in a time-dependent manner and that of TGF-β was clearly induced only on day 28 after treatment (Fig. 5). The concentration of inducible nitric oxide synthatase (iNOS) increased significantly during the experimental period and peaked on day 7 after treatment, whereas expression of COX-2 was upregulated from day 1 to day 14 after treatment. The effect of DEP exposure on the expression of mesothelin protein was minimal. The protein expression levels of p-IκBα, p-STAT3, and NFκB p65 peaked on day 14 after treatment.

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

Show MeSH
Related in: MedlinePlus