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Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

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Related in: MedlinePlus

Images of cells in BAL fluid after a single instillation of DEPs.Mice were administered a single intratracheal instillation of DEPs at a dose of 10 mg/kg, and BAL fluid samples were harvested on the designated days: (A) control, (B) 7 days, (C) 14 days, and (D) 28 days after exposure. The results were confirmed from all the mice used, and representative images are shown.
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pone-0026749-g004: Images of cells in BAL fluid after a single instillation of DEPs.Mice were administered a single intratracheal instillation of DEPs at a dose of 10 mg/kg, and BAL fluid samples were harvested on the designated days: (A) control, (B) 7 days, (C) 14 days, and (D) 28 days after exposure. The results were confirmed from all the mice used, and representative images are shown.

Mentions: Figure 4 shows an image of cells in the BAL fluid harvested at each time point. As shown in Figure 3, DEPs were suspended in BAL fluid until day 14 after treatment and were partially distributed in the cytoplasm of cells. Phagocytosis by immune cells in BAL fluid was completed on day 28 after treatment, which was indicated by the mainly cytoplasmic and partially nuclear distribution.


Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Images of cells in BAL fluid after a single instillation of DEPs.Mice were administered a single intratracheal instillation of DEPs at a dose of 10 mg/kg, and BAL fluid samples were harvested on the designated days: (A) control, (B) 7 days, (C) 14 days, and (D) 28 days after exposure. The results were confirmed from all the mice used, and representative images are shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198839&req=5

pone-0026749-g004: Images of cells in BAL fluid after a single instillation of DEPs.Mice were administered a single intratracheal instillation of DEPs at a dose of 10 mg/kg, and BAL fluid samples were harvested on the designated days: (A) control, (B) 7 days, (C) 14 days, and (D) 28 days after exposure. The results were confirmed from all the mice used, and representative images are shown.
Mentions: Figure 4 shows an image of cells in the BAL fluid harvested at each time point. As shown in Figure 3, DEPs were suspended in BAL fluid until day 14 after treatment and were partially distributed in the cytoplasm of cells. Phagocytosis by immune cells in BAL fluid was completed on day 28 after treatment, which was indicated by the mainly cytoplasmic and partially nuclear distribution.

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

Show MeSH
Related in: MedlinePlus