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Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

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Related in: MedlinePlus

Changes in body weight after a single instillation of DEPs.Body weight of mice (n = 16) in the control and day 28 groups before exposure and on days 1, 7, 14, and 28 after exposure. *P<0.05.
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pone-0026749-g002: Changes in body weight after a single instillation of DEPs.Body weight of mice (n = 16) in the control and day 28 groups before exposure and on days 1, 7, 14, and 28 after exposure. *P<0.05.

Mentions: Before exposure to DEPs, the mean body weight in the control and DEP-treated groups were 32.4±1.3 g and 32.4±1.4 g, respectively (Fig 2). On day 28 after treatment, the mean body weight in the DEP-treated group (37.5±2.38 g) was significantly lesser than that in the control group (38.8±2.48 g).


Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.

Park EJ, Roh J, Kang MS, Kim SN, Kim Y, Choi S - PLoS ONE (2011)

Changes in body weight after a single instillation of DEPs.Body weight of mice (n = 16) in the control and day 28 groups before exposure and on days 1, 7, 14, and 28 after exposure. *P<0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198839&req=5

pone-0026749-g002: Changes in body weight after a single instillation of DEPs.Body weight of mice (n = 16) in the control and day 28 groups before exposure and on days 1, 7, 14, and 28 after exposure. *P<0.05.
Mentions: Before exposure to DEPs, the mean body weight in the control and DEP-treated groups were 32.4±1.3 g and 32.4±1.4 g, respectively (Fig 2). On day 28 after treatment, the mean body weight in the DEP-treated group (37.5±2.38 g) was significantly lesser than that in the control group (38.8±2.48 g).

Bottom Line: Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response.Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs.Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

ABSTRACT
Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.

Show MeSH
Related in: MedlinePlus