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Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats.

Li K, Diao Y, Zhang H, Wang S, Zhang Z, Yu B, Huang S, Yang H - BMC Complement Altern Med (2011)

Bottom Line: After optimal extraction and purification, content of tannin extracts was increased to 81%.Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro.The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116029, PR China.

ABSTRACT

Background: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.

Methods: The content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group Ⅰ, Ⅱ and Ⅲ were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.

Results: After optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of group Ⅱ was higher than that of group Ⅰ. After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of group Ⅱ was found to be better than that of group Ⅰ in terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of group Ⅱ was higher than that of group Ⅰ.

Conclusion: The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

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VEGFA mRNA expression on day 3. (*P < 0.05 as compared with groupⅠ)
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Figure 5: VEGFA mRNA expression on day 3. (*P < 0.05 as compared with groupⅠ)

Mentions: On the third day, VEGFA mRNA expression of the wound was observed among all three groups after determining it through the RT-PCR technique (Figure 5). The expression of both group II and group III was higher than that of group I (P < 0.05). At other time points, no significant difference was reported in the expression among the three groups.


Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats.

Li K, Diao Y, Zhang H, Wang S, Zhang Z, Yu B, Huang S, Yang H - BMC Complement Altern Med (2011)

VEGFA mRNA expression on day 3. (*P < 0.05 as compared with groupⅠ)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198757&req=5

Figure 5: VEGFA mRNA expression on day 3. (*P < 0.05 as compared with groupⅠ)
Mentions: On the third day, VEGFA mRNA expression of the wound was observed among all three groups after determining it through the RT-PCR technique (Figure 5). The expression of both group II and group III was higher than that of group I (P < 0.05). At other time points, no significant difference was reported in the expression among the three groups.

Bottom Line: After optimal extraction and purification, content of tannin extracts was increased to 81%.Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro.The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116029, PR China.

ABSTRACT

Background: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.

Methods: The content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group Ⅰ, Ⅱ and Ⅲ were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.

Results: After optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of group Ⅱ was higher than that of group Ⅰ. After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of group Ⅱ was found to be better than that of group Ⅰ in terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of group Ⅱ was higher than that of group Ⅰ.

Conclusion: The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

Show MeSH
Related in: MedlinePlus