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Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats.

Li K, Diao Y, Zhang H, Wang S, Zhang Z, Yu B, Huang S, Yang H - BMC Complement Altern Med (2011)

Bottom Line: After optimal extraction and purification, content of tannin extracts was increased to 81%.Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro.The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116029, PR China.

ABSTRACT

Background: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.

Methods: The content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group Ⅰ, Ⅱ and Ⅲ were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.

Results: After optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of group Ⅱ was higher than that of group Ⅰ. After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of group Ⅱ was found to be better than that of group Ⅰ in terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of group Ⅱ was higher than that of group Ⅰ.

Conclusion: The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

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Morphology of Staphylococcus aureus and Klebsiella Pneumonia under scanning electron microscope. A the normal morphology of Staphylococcus aureus; B the morphology of Staphylococcus aureus on 24 h; C the normal morphology of Klebsiella Pneumonia; D the morphology of Klebsiella Pneumonia on 24 h
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Figure 2: Morphology of Staphylococcus aureus and Klebsiella Pneumonia under scanning electron microscope. A the normal morphology of Staphylococcus aureus; B the morphology of Staphylococcus aureus on 24 h; C the normal morphology of Klebsiella Pneumonia; D the morphology of Klebsiella Pneumonia on 24 h

Mentions: After treating the tannin extract, we observed that the Staphylococcus aureus and Klebsiella pneumonia had morphologic changes under electron microscope. The changes were obviously compared with the bacteria of the control group. A normal bacterium shows smooth surface, plump appearance, and good refraction. At 24 hours after treatment, majority of the bacteria of group II became obviously shrank, dry, and distorted. Some bacteria became obviously depressed, vesicular, or irregular. The changes showed that tannin extracts of a MIC concentration could obviously destroy those two kinds of bacteria. Results have been shown in Figure 2.


Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats.

Li K, Diao Y, Zhang H, Wang S, Zhang Z, Yu B, Huang S, Yang H - BMC Complement Altern Med (2011)

Morphology of Staphylococcus aureus and Klebsiella Pneumonia under scanning electron microscope. A the normal morphology of Staphylococcus aureus; B the morphology of Staphylococcus aureus on 24 h; C the normal morphology of Klebsiella Pneumonia; D the morphology of Klebsiella Pneumonia on 24 h
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198757&req=5

Figure 2: Morphology of Staphylococcus aureus and Klebsiella Pneumonia under scanning electron microscope. A the normal morphology of Staphylococcus aureus; B the morphology of Staphylococcus aureus on 24 h; C the normal morphology of Klebsiella Pneumonia; D the morphology of Klebsiella Pneumonia on 24 h
Mentions: After treating the tannin extract, we observed that the Staphylococcus aureus and Klebsiella pneumonia had morphologic changes under electron microscope. The changes were obviously compared with the bacteria of the control group. A normal bacterium shows smooth surface, plump appearance, and good refraction. At 24 hours after treatment, majority of the bacteria of group II became obviously shrank, dry, and distorted. Some bacteria became obviously depressed, vesicular, or irregular. The changes showed that tannin extracts of a MIC concentration could obviously destroy those two kinds of bacteria. Results have been shown in Figure 2.

Bottom Line: After optimal extraction and purification, content of tannin extracts was increased to 81%.Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro.The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116029, PR China.

ABSTRACT

Background: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.

Methods: The content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group Ⅰ, Ⅱ and Ⅲ were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.

Results: After optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of group Ⅱ was higher than that of group Ⅰ. After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of group Ⅱ was found to be better than that of group Ⅰ in terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of group Ⅱ was higher than that of group Ⅰ.

Conclusion: The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

Show MeSH
Related in: MedlinePlus