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Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats.

Li K, Diao Y, Zhang H, Wang S, Zhang Z, Yu B, Huang S, Yang H - BMC Complement Altern Med (2011)

Bottom Line: After optimal extraction and purification, content of tannin extracts was increased to 81%.Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro.The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116029, PR China.

ABSTRACT

Background: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.

Methods: The content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group Ⅰ, Ⅱ and Ⅲ were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.

Results: After optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of group Ⅱ was higher than that of group Ⅰ. After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of group Ⅱ was found to be better than that of group Ⅰ in terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of group Ⅱ was higher than that of group Ⅰ.

Conclusion: The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

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The calibration curve of gallic acid. linear range (1~10 μg/ml), Regression equation(Y = aX + b): y = 52.496x + 0.0163, R2 = 0.9993(n = 6)
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Figure 1: The calibration curve of gallic acid. linear range (1~10 μg/ml), Regression equation(Y = aX + b): y = 52.496x + 0.0163, R2 = 0.9993(n = 6)

Mentions: Regression equation was calculated in the form Y = aX + b. In the formula, X and Y are concentration of standard solution (mg/mL) and corresponding absorbance. a and b correspond to slope and intercept, respectively. A calibration curve of gallic acid (ranging from 1 to 10 μg/ml) was prepared and listed in Figure 1. According to the OEPT, the content of tannin extracts was estimated to be 81%.


Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats.

Li K, Diao Y, Zhang H, Wang S, Zhang Z, Yu B, Huang S, Yang H - BMC Complement Altern Med (2011)

The calibration curve of gallic acid. linear range (1~10 μg/ml), Regression equation(Y = aX + b): y = 52.496x + 0.0163, R2 = 0.9993(n = 6)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198757&req=5

Figure 1: The calibration curve of gallic acid. linear range (1~10 μg/ml), Regression equation(Y = aX + b): y = 52.496x + 0.0163, R2 = 0.9993(n = 6)
Mentions: Regression equation was calculated in the form Y = aX + b. In the formula, X and Y are concentration of standard solution (mg/mL) and corresponding absorbance. a and b correspond to slope and intercept, respectively. A calibration curve of gallic acid (ranging from 1 to 10 μg/ml) was prepared and listed in Figure 1. According to the OEPT, the content of tannin extracts was estimated to be 81%.

Bottom Line: After optimal extraction and purification, content of tannin extracts was increased to 81%.Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro.The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116029, PR China.

ABSTRACT

Background: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.

Methods: The content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group Ⅰ, Ⅱ and Ⅲ were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.

Results: After optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of group Ⅱ was higher than that of group Ⅰ. After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of group Ⅱ was found to be better than that of group Ⅰ in terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of group Ⅱ was higher than that of group Ⅰ.

Conclusion: The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

Show MeSH
Related in: MedlinePlus