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Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn AN, Rueschhoff EE, Franks RG - PLoS ONE (2011)

Bottom Line: Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM.This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation.Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

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Related in: MedlinePlus

Results of in situ hybridization with At1G68780 antisense probe.Panels A–C show longitudinal tissue sections; panels D-H show transverse (cross) sections. Numbers indicate floral stages. Arrowhead indicates medial domain expression. s - sepal primordia; p - petal primordia; ga - gynoecial apex; ifm - inflorescence meristem. Scale bars in all panels are 100 microns. All panels are Col-0 wild type except as noted.
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pone-0026231-g015: Results of in situ hybridization with At1G68780 antisense probe.Panels A–C show longitudinal tissue sections; panels D-H show transverse (cross) sections. Numbers indicate floral stages. Arrowhead indicates medial domain expression. s - sepal primordia; p - petal primordia; ga - gynoecial apex; ifm - inflorescence meristem. Scale bars in all panels are 100 microns. All panels are Col-0 wild type except as noted.

Mentions: The gene At1G68780 is annotated as a member of the RNase inhibitor-like superfamily containing multiple leucine rich repeat InterPro domains (InterPro:IPR001611) [60]. Expression of At1G68780 was detected weakly throughout the inflorescence meristem and floral stages 1–2 (Fig. 15A). During floral stage 3 expression was strongly detected within the sepal primordia. During floral stages 6 through 8, At1G68780 is most strongly detected in apical portions of the gynoecium throughout both medial and lateral domains (Figs. 15B through 15D). Gynoecial expression was significantly reduced in the stage 7 seu ant double mutant gynoecia. Expression was detected in wild type flowers throughout petal development during floral stages 5 through 12 (Figs. 15B, C, G and data not shown). Expression within the petals was reduced in the ant single mutant relative to wild type at stage 11 (Fig. 15H).


Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn AN, Rueschhoff EE, Franks RG - PLoS ONE (2011)

Results of in situ hybridization with At1G68780 antisense probe.Panels A–C show longitudinal tissue sections; panels D-H show transverse (cross) sections. Numbers indicate floral stages. Arrowhead indicates medial domain expression. s - sepal primordia; p - petal primordia; ga - gynoecial apex; ifm - inflorescence meristem. Scale bars in all panels are 100 microns. All panels are Col-0 wild type except as noted.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198736&req=5

pone-0026231-g015: Results of in situ hybridization with At1G68780 antisense probe.Panels A–C show longitudinal tissue sections; panels D-H show transverse (cross) sections. Numbers indicate floral stages. Arrowhead indicates medial domain expression. s - sepal primordia; p - petal primordia; ga - gynoecial apex; ifm - inflorescence meristem. Scale bars in all panels are 100 microns. All panels are Col-0 wild type except as noted.
Mentions: The gene At1G68780 is annotated as a member of the RNase inhibitor-like superfamily containing multiple leucine rich repeat InterPro domains (InterPro:IPR001611) [60]. Expression of At1G68780 was detected weakly throughout the inflorescence meristem and floral stages 1–2 (Fig. 15A). During floral stage 3 expression was strongly detected within the sepal primordia. During floral stages 6 through 8, At1G68780 is most strongly detected in apical portions of the gynoecium throughout both medial and lateral domains (Figs. 15B through 15D). Gynoecial expression was significantly reduced in the stage 7 seu ant double mutant gynoecia. Expression was detected in wild type flowers throughout petal development during floral stages 5 through 12 (Figs. 15B, C, G and data not shown). Expression within the petals was reduced in the ant single mutant relative to wild type at stage 11 (Fig. 15H).

Bottom Line: Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM.This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation.Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

Show MeSH
Related in: MedlinePlus