Limits...
Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn AN, Rueschhoff EE, Franks RG - PLoS ONE (2011)

Bottom Line: Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM.This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation.Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

Show MeSH

Related in: MedlinePlus

Results of in situ hybridization with AT5G57720 (REM15) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression. ov - ovule; st- stamen primordia; mmc - megaspore mother cell. Scale bars in all panels are 50 microns. All panels are Col-0 wild type tissue.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3198736&req=5

pone-0026231-g007: Results of in situ hybridization with AT5G57720 (REM15) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression. ov - ovule; st- stamen primordia; mmc - megaspore mother cell. Scale bars in all panels are 50 microns. All panels are Col-0 wild type tissue.

Mentions: Expression of AT5G57720 (REM15) in stamen and carpel primordia was previously reported [43]. We first detect expression of AT5G57720 during early stage 4 as a ring of expression that appears to mark whorl three positions just interior or adaxial to the sepal primordia (Fig. 7A). During stage 6 AT5G57720 (REM15) is detected in the gynoecium in the medial domain, most strongly in abaxial positions (Fig. 7B). During stage 7 AT5G57720 (REM15) is detected throughout the medial domain of the gynoecium (Fig. 7C) and continues to be detected in adaxial portions of the medial domain during stage 8 (Fig. 7 D and E). AT5G57720 (REM15) is detected in ovule primordia as they arise during stage 8 (Fig. 7F) and continues to be expressed in the megaspore mother cell and in nucellar portions of the ovule through stage 12 (Fig. 7G and H). AT5G57720 (REM15) is also detected strongly in stamen tapetal cells during stage 9 (data not shown).


Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn AN, Rueschhoff EE, Franks RG - PLoS ONE (2011)

Results of in situ hybridization with AT5G57720 (REM15) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression. ov - ovule; st- stamen primordia; mmc - megaspore mother cell. Scale bars in all panels are 50 microns. All panels are Col-0 wild type tissue.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198736&req=5

pone-0026231-g007: Results of in situ hybridization with AT5G57720 (REM15) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression. ov - ovule; st- stamen primordia; mmc - megaspore mother cell. Scale bars in all panels are 50 microns. All panels are Col-0 wild type tissue.
Mentions: Expression of AT5G57720 (REM15) in stamen and carpel primordia was previously reported [43]. We first detect expression of AT5G57720 during early stage 4 as a ring of expression that appears to mark whorl three positions just interior or adaxial to the sepal primordia (Fig. 7A). During stage 6 AT5G57720 (REM15) is detected in the gynoecium in the medial domain, most strongly in abaxial positions (Fig. 7B). During stage 7 AT5G57720 (REM15) is detected throughout the medial domain of the gynoecium (Fig. 7C) and continues to be detected in adaxial portions of the medial domain during stage 8 (Fig. 7 D and E). AT5G57720 (REM15) is detected in ovule primordia as they arise during stage 8 (Fig. 7F) and continues to be expressed in the megaspore mother cell and in nucellar portions of the ovule through stage 12 (Fig. 7G and H). AT5G57720 (REM15) is also detected strongly in stamen tapetal cells during stage 9 (data not shown).

Bottom Line: Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM.This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation.Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

Show MeSH
Related in: MedlinePlus