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Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn AN, Rueschhoff EE, Franks RG - PLoS ONE (2011)

Bottom Line: Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM.This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation.Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

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Related in: MedlinePlus

Results of in situ hybridization with AT5G18000 (VDD) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression; ov - ovule; t- tapetal cells. Scale bar in J represents 100 microns for all panels. All panels are Col-0 wild type tissue unless otherwise indicated.
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pone-0026231-g004: Results of in situ hybridization with AT5G18000 (VDD) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression; ov - ovule; t- tapetal cells. Scale bar in J represents 100 microns for all panels. All panels are Col-0 wild type tissue unless otherwise indicated.

Mentions: Expression of AT5G18000 (VDD) was previously reported in inflorescence and floral meristems and in ovules, as well as within the developing female gametophyte [41]. Within the gynoecium we first observe expression of VDD at stage 7 when it is detected weakly throughout the primordium (Fig. 4A). Expression was stronger at the medial portions of the apex of the stage 7 and 8 gynoecial primordia relative to more basal positions (Fig. 4B, C). Expression continues in the apical medial domain into stage 10 (Fig. 4G). Expression is also detected in ovule primordia as they arise at stage 8 and continues throughout ovule development (Fig. 4E and data not shown) [41]. Expression in ovule primordia at stage 11 was strongest in the chalazal portions of the ovule. Expression in the ant single mutant tissue at this stage appeared reduced, suggesting that ANT may regulate the expression of VDD in the chalazal portions of the ovule. Strong expression was also detected in tapetal cells of the anther at stage 9 (Fig. 4H).


Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn AN, Rueschhoff EE, Franks RG - PLoS ONE (2011)

Results of in situ hybridization with AT5G18000 (VDD) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression; ov - ovule; t- tapetal cells. Scale bar in J represents 100 microns for all panels. All panels are Col-0 wild type tissue unless otherwise indicated.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198736&req=5

pone-0026231-g004: Results of in situ hybridization with AT5G18000 (VDD) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression; ov - ovule; t- tapetal cells. Scale bar in J represents 100 microns for all panels. All panels are Col-0 wild type tissue unless otherwise indicated.
Mentions: Expression of AT5G18000 (VDD) was previously reported in inflorescence and floral meristems and in ovules, as well as within the developing female gametophyte [41]. Within the gynoecium we first observe expression of VDD at stage 7 when it is detected weakly throughout the primordium (Fig. 4A). Expression was stronger at the medial portions of the apex of the stage 7 and 8 gynoecial primordia relative to more basal positions (Fig. 4B, C). Expression continues in the apical medial domain into stage 10 (Fig. 4G). Expression is also detected in ovule primordia as they arise at stage 8 and continues throughout ovule development (Fig. 4E and data not shown) [41]. Expression in ovule primordia at stage 11 was strongest in the chalazal portions of the ovule. Expression in the ant single mutant tissue at this stage appeared reduced, suggesting that ANT may regulate the expression of VDD in the chalazal portions of the ovule. Strong expression was also detected in tapetal cells of the anther at stage 9 (Fig. 4H).

Bottom Line: Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM.This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation.Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

Show MeSH
Related in: MedlinePlus