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Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn AN, Rueschhoff EE, Franks RG - PLoS ONE (2011)

Bottom Line: Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM.This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation.Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

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Related in: MedlinePlus

Results of in situ hybridization with At4G31610 (REM34/AtREM1) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression; ov - ovule. All scale bars are 100 microns. All panels are Col-0 wild type tissue unless otherwise indicated.
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pone-0026231-g003: Results of in situ hybridization with At4G31610 (REM34/AtREM1) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression; ov - ovule. All scale bars are 100 microns. All panels are Col-0 wild type tissue unless otherwise indicated.

Mentions: Expression of At4G31610 (REM34/AtREM1) in the inflorescence meristem and in floral stages 2–5 has been previously reported [42]. Franco-Zorrilla et al. also report expression of AT4G31610 (REM34/AtREM1) is confined to gynoecial primordium from stage 6 onward and later expressed in the medial ridge, septum, style and stigma [42]. Our analysis of gynoecial expression patterns reveals that At4G31610 (REM34/AtREM1) is expressed in the adaxial core of the stage 6 and stage 7 gynoecial primordia (Fig. 3C and D). During stage 7 and 8 expression is strongest at the apex of the gynoecium in the medial domain (Fig. 3E, G). During late stage 8 expression is detected in the ovule anlagen (Fig. 3H) while by stage 9 expression appears to be restricted to the cells that lie between the ovule primordia and are likely to be the progenitors of the gynoecial septum. Expression in the seu ant double mutant tissue appeared reduced in the gynoecial primordia during stages 6–10 but was similar to wild type during stage 2–3 (Fig. 3 A, B, J and L).


Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn AN, Rueschhoff EE, Franks RG - PLoS ONE (2011)

Results of in situ hybridization with At4G31610 (REM34/AtREM1) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression; ov - ovule. All scale bars are 100 microns. All panels are Col-0 wild type tissue unless otherwise indicated.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198736&req=5

pone-0026231-g003: Results of in situ hybridization with At4G31610 (REM34/AtREM1) antisense probe.Numbers indicate floral stages. All panels show transverse (cross sectional) tissue orientation. Arrowheads indicate medial domain expression; ov - ovule. All scale bars are 100 microns. All panels are Col-0 wild type tissue unless otherwise indicated.
Mentions: Expression of At4G31610 (REM34/AtREM1) in the inflorescence meristem and in floral stages 2–5 has been previously reported [42]. Franco-Zorrilla et al. also report expression of AT4G31610 (REM34/AtREM1) is confined to gynoecial primordium from stage 6 onward and later expressed in the medial ridge, septum, style and stigma [42]. Our analysis of gynoecial expression patterns reveals that At4G31610 (REM34/AtREM1) is expressed in the adaxial core of the stage 6 and stage 7 gynoecial primordia (Fig. 3C and D). During stage 7 and 8 expression is strongest at the apex of the gynoecium in the medial domain (Fig. 3E, G). During late stage 8 expression is detected in the ovule anlagen (Fig. 3H) while by stage 9 expression appears to be restricted to the cells that lie between the ovule primordia and are likely to be the progenitors of the gynoecial septum. Expression in the seu ant double mutant tissue appeared reduced in the gynoecial primordia during stages 6–10 but was similar to wild type during stage 2–3 (Fig. 3 A, B, J and L).

Bottom Line: Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM.This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation.Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

Show MeSH
Related in: MedlinePlus