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Decreased expression of dual-specificity phosphatase 9 is associated with poor prognosis in clear cell renal cell carcinoma.

Wu S, Wang Y, Sun L, Zhang Z, Jiang Z, Qin Z, Han H, Liu Z, Li X, Tang A, Gui Y, Cai Z, Zhou F - BMC Cancer (2011)

Bottom Line: The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001).We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC.DUSP-9 may represent a novel and useful prognostic marker for ccRCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, PR China.

ABSTRACT

Background: The molecular mechanisms involved in the development and progression of clear cell renal cell carcinomas (ccRCCs) are poorly understood. The objective of this study was to analyze the expression of dual-specificity phosphatase 9 (DUSP-9) and determine its clinical significance in human ccRCCs.

Methods: The expression of DUSP-9 mRNA was determined in 46 paired samples of ccRCCs and adjacent normal tissues by using real-time qPCR. The expression of the DUSP-9 was determined in 211 samples of ccRCCs and 107 paired samples of adjacent normal tissues by immunohistochemical analysis. Statistical analysis was performed to define the relationship between the expression of DUSP-9 and the clinical features of ccRCC.

Results: The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). An immunohistochemical analysis of 107 paired tissue specimens showed that the DUSP-9 expression was lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). Moreover, there was a significant correlation between the DUSP-9 expression in ccRCCs and gender (p = 0.031), tumor size (p = 0.001), pathologic stage (p = 0.001), Fuhrman grade (p = 0.002), T stage (p = 0.001), N classification (p = 0.012), metastasis (p = 0.005), and recurrence (p < 0.001). Patients with lower DUSP-9 expression had shorter overall survival time than those with higher DUSP-9 expression (p < 0.001). Multivariate analysis indicated that low expression of the DUSP-9 was an independent predictor for poor survival of ccRCC patients.

Conclusion: To our knowledge, this is the first study that determines the relationship between DUSP-9 expression and prognosis in ccRCC. We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC. DUSP-9 may represent a novel and useful prognostic marker for ccRCC.

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Related in: MedlinePlus

Decreased protein expression of DUSP-9 in ccRCC. The relative protein expression of DUSP-9 in ccRCC tumor (T) tissue samples was lower than that in the paired adjacent normal (N) tissue samples (n = 107, P < 0.001). The bottom and top of the box are the lower and upper quartiles, and the band near the middle of the box is the median. The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile. Four black spots represent the special value outliers.
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Figure 2: Decreased protein expression of DUSP-9 in ccRCC. The relative protein expression of DUSP-9 in ccRCC tumor (T) tissue samples was lower than that in the paired adjacent normal (N) tissue samples (n = 107, P < 0.001). The bottom and top of the box are the lower and upper quartiles, and the band near the middle of the box is the median. The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile. Four black spots represent the special value outliers.

Mentions: Expression and subcellular localization of protein were determined by immunohistochemical analysis in 107 paraffin-embedded ccRCC tissues and 107 paired specimens of adjacent normal tissues (Figure 2). In normal renal tissue, specific DUSP-9 was localized mainly in the cytoplasm of renal cells in the form of yellow-brown granules (Figure 3A). The DUSP-9 protein expression in the 102 tumor tissue samples was lower than that in the adjacent normal tissue samples (p < 0.001, paired-sample t test, Figure 3B).


Decreased expression of dual-specificity phosphatase 9 is associated with poor prognosis in clear cell renal cell carcinoma.

Wu S, Wang Y, Sun L, Zhang Z, Jiang Z, Qin Z, Han H, Liu Z, Li X, Tang A, Gui Y, Cai Z, Zhou F - BMC Cancer (2011)

Decreased protein expression of DUSP-9 in ccRCC. The relative protein expression of DUSP-9 in ccRCC tumor (T) tissue samples was lower than that in the paired adjacent normal (N) tissue samples (n = 107, P < 0.001). The bottom and top of the box are the lower and upper quartiles, and the band near the middle of the box is the median. The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile. Four black spots represent the special value outliers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198720&req=5

Figure 2: Decreased protein expression of DUSP-9 in ccRCC. The relative protein expression of DUSP-9 in ccRCC tumor (T) tissue samples was lower than that in the paired adjacent normal (N) tissue samples (n = 107, P < 0.001). The bottom and top of the box are the lower and upper quartiles, and the band near the middle of the box is the median. The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile. Four black spots represent the special value outliers.
Mentions: Expression and subcellular localization of protein were determined by immunohistochemical analysis in 107 paraffin-embedded ccRCC tissues and 107 paired specimens of adjacent normal tissues (Figure 2). In normal renal tissue, specific DUSP-9 was localized mainly in the cytoplasm of renal cells in the form of yellow-brown granules (Figure 3A). The DUSP-9 protein expression in the 102 tumor tissue samples was lower than that in the adjacent normal tissue samples (p < 0.001, paired-sample t test, Figure 3B).

Bottom Line: The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001).We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC.DUSP-9 may represent a novel and useful prognostic marker for ccRCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, PR China.

ABSTRACT

Background: The molecular mechanisms involved in the development and progression of clear cell renal cell carcinomas (ccRCCs) are poorly understood. The objective of this study was to analyze the expression of dual-specificity phosphatase 9 (DUSP-9) and determine its clinical significance in human ccRCCs.

Methods: The expression of DUSP-9 mRNA was determined in 46 paired samples of ccRCCs and adjacent normal tissues by using real-time qPCR. The expression of the DUSP-9 was determined in 211 samples of ccRCCs and 107 paired samples of adjacent normal tissues by immunohistochemical analysis. Statistical analysis was performed to define the relationship between the expression of DUSP-9 and the clinical features of ccRCC.

Results: The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). An immunohistochemical analysis of 107 paired tissue specimens showed that the DUSP-9 expression was lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). Moreover, there was a significant correlation between the DUSP-9 expression in ccRCCs and gender (p = 0.031), tumor size (p = 0.001), pathologic stage (p = 0.001), Fuhrman grade (p = 0.002), T stage (p = 0.001), N classification (p = 0.012), metastasis (p = 0.005), and recurrence (p < 0.001). Patients with lower DUSP-9 expression had shorter overall survival time than those with higher DUSP-9 expression (p < 0.001). Multivariate analysis indicated that low expression of the DUSP-9 was an independent predictor for poor survival of ccRCC patients.

Conclusion: To our knowledge, this is the first study that determines the relationship between DUSP-9 expression and prognosis in ccRCC. We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC. DUSP-9 may represent a novel and useful prognostic marker for ccRCC.

Show MeSH
Related in: MedlinePlus