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Decreased expression of dual-specificity phosphatase 9 is associated with poor prognosis in clear cell renal cell carcinoma.

Wu S, Wang Y, Sun L, Zhang Z, Jiang Z, Qin Z, Han H, Liu Z, Li X, Tang A, Gui Y, Cai Z, Zhou F - BMC Cancer (2011)

Bottom Line: The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001).We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC.DUSP-9 may represent a novel and useful prognostic marker for ccRCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, PR China.

ABSTRACT

Background: The molecular mechanisms involved in the development and progression of clear cell renal cell carcinomas (ccRCCs) are poorly understood. The objective of this study was to analyze the expression of dual-specificity phosphatase 9 (DUSP-9) and determine its clinical significance in human ccRCCs.

Methods: The expression of DUSP-9 mRNA was determined in 46 paired samples of ccRCCs and adjacent normal tissues by using real-time qPCR. The expression of the DUSP-9 was determined in 211 samples of ccRCCs and 107 paired samples of adjacent normal tissues by immunohistochemical analysis. Statistical analysis was performed to define the relationship between the expression of DUSP-9 and the clinical features of ccRCC.

Results: The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). An immunohistochemical analysis of 107 paired tissue specimens showed that the DUSP-9 expression was lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). Moreover, there was a significant correlation between the DUSP-9 expression in ccRCCs and gender (p = 0.031), tumor size (p = 0.001), pathologic stage (p = 0.001), Fuhrman grade (p = 0.002), T stage (p = 0.001), N classification (p = 0.012), metastasis (p = 0.005), and recurrence (p < 0.001). Patients with lower DUSP-9 expression had shorter overall survival time than those with higher DUSP-9 expression (p < 0.001). Multivariate analysis indicated that low expression of the DUSP-9 was an independent predictor for poor survival of ccRCC patients.

Conclusion: To our knowledge, this is the first study that determines the relationship between DUSP-9 expression and prognosis in ccRCC. We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC. DUSP-9 may represent a novel and useful prognostic marker for ccRCC.

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Real-time quantitative RT-PCR analysis of DUSP-9 expression. The relative expression of DUSP-9 mRNA in RCC tumor tissue samples was lower than that in the paired adjacent normal (N) tissue samples (n = 46, P < 0.001). The bottom and the top of the box represent the 25th and the 75th percentile, respectively, and the band near the middle of the box is the 50th percentile (the median). The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile.
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Figure 1: Real-time quantitative RT-PCR analysis of DUSP-9 expression. The relative expression of DUSP-9 mRNA in RCC tumor tissue samples was lower than that in the paired adjacent normal (N) tissue samples (n = 46, P < 0.001). The bottom and the top of the box represent the 25th and the 75th percentile, respectively, and the band near the middle of the box is the 50th percentile (the median). The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile.

Mentions: The transcription level of DUSP-9 was determined by quantitative RT-PCR assays of 46 ccRCC tumor samples and the paired adjacent normal tissue samples. In 45 tumor samples, the mRNA level of DUSP-9 was significantly lower than that in the adjacent normal tissue sample (p < 0.001, paired-sample t tests, Figure 1).


Decreased expression of dual-specificity phosphatase 9 is associated with poor prognosis in clear cell renal cell carcinoma.

Wu S, Wang Y, Sun L, Zhang Z, Jiang Z, Qin Z, Han H, Liu Z, Li X, Tang A, Gui Y, Cai Z, Zhou F - BMC Cancer (2011)

Real-time quantitative RT-PCR analysis of DUSP-9 expression. The relative expression of DUSP-9 mRNA in RCC tumor tissue samples was lower than that in the paired adjacent normal (N) tissue samples (n = 46, P < 0.001). The bottom and the top of the box represent the 25th and the 75th percentile, respectively, and the band near the middle of the box is the 50th percentile (the median). The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198720&req=5

Figure 1: Real-time quantitative RT-PCR analysis of DUSP-9 expression. The relative expression of DUSP-9 mRNA in RCC tumor tissue samples was lower than that in the paired adjacent normal (N) tissue samples (n = 46, P < 0.001). The bottom and the top of the box represent the 25th and the 75th percentile, respectively, and the band near the middle of the box is the 50th percentile (the median). The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile.
Mentions: The transcription level of DUSP-9 was determined by quantitative RT-PCR assays of 46 ccRCC tumor samples and the paired adjacent normal tissue samples. In 45 tumor samples, the mRNA level of DUSP-9 was significantly lower than that in the adjacent normal tissue sample (p < 0.001, paired-sample t tests, Figure 1).

Bottom Line: The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001).We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC.DUSP-9 may represent a novel and useful prognostic marker for ccRCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, PR China.

ABSTRACT

Background: The molecular mechanisms involved in the development and progression of clear cell renal cell carcinomas (ccRCCs) are poorly understood. The objective of this study was to analyze the expression of dual-specificity phosphatase 9 (DUSP-9) and determine its clinical significance in human ccRCCs.

Methods: The expression of DUSP-9 mRNA was determined in 46 paired samples of ccRCCs and adjacent normal tissues by using real-time qPCR. The expression of the DUSP-9 was determined in 211 samples of ccRCCs and 107 paired samples of adjacent normal tissues by immunohistochemical analysis. Statistical analysis was performed to define the relationship between the expression of DUSP-9 and the clinical features of ccRCC.

Results: The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). An immunohistochemical analysis of 107 paired tissue specimens showed that the DUSP-9 expression was lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). Moreover, there was a significant correlation between the DUSP-9 expression in ccRCCs and gender (p = 0.031), tumor size (p = 0.001), pathologic stage (p = 0.001), Fuhrman grade (p = 0.002), T stage (p = 0.001), N classification (p = 0.012), metastasis (p = 0.005), and recurrence (p < 0.001). Patients with lower DUSP-9 expression had shorter overall survival time than those with higher DUSP-9 expression (p < 0.001). Multivariate analysis indicated that low expression of the DUSP-9 was an independent predictor for poor survival of ccRCC patients.

Conclusion: To our knowledge, this is the first study that determines the relationship between DUSP-9 expression and prognosis in ccRCC. We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC. DUSP-9 may represent a novel and useful prognostic marker for ccRCC.

Show MeSH
Related in: MedlinePlus