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RMRP is a non-coding RNA essential for early murine development.

Rosenbluh J, Nijhawan D, Chen Z, Wong KK, Masutomi K, Hahn WC - PLoS ONE (2011)

Bottom Line: RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia.To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional).We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, United States of America.

ABSTRACT
RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP mice, indicating that RMRP is essential for early embryonic development.

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Targeting of murine RMRP.A. Murine targeting vector (MTV) B. Southern blot of ES cells following selection for alleles with integrated MTV (the southern probe is shown in figure 1a) C. PCR analysis of RC (RMRP conditional) pups D. PCR analysis of pups derived from the interbreeding of RC mice and mice expressing CMV-Cre.
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pone-0026270-g001: Targeting of murine RMRP.A. Murine targeting vector (MTV) B. Southern blot of ES cells following selection for alleles with integrated MTV (the southern probe is shown in figure 1a) C. PCR analysis of RC (RMRP conditional) pups D. PCR analysis of pups derived from the interbreeding of RC mice and mice expressing CMV-Cre.

Mentions: We created a targeting vector specific for murine RMRP using the pEasyflox backbone [7]. The targeting vector contains the RMRP gene and promoter (800 bp up stream of murine RMRP [1]) flanked by two loxP sequences. A neomycin selectable marker flanked by two loxP sequences was placed upstream of RMRP (Figure 1A).


RMRP is a non-coding RNA essential for early murine development.

Rosenbluh J, Nijhawan D, Chen Z, Wong KK, Masutomi K, Hahn WC - PLoS ONE (2011)

Targeting of murine RMRP.A. Murine targeting vector (MTV) B. Southern blot of ES cells following selection for alleles with integrated MTV (the southern probe is shown in figure 1a) C. PCR analysis of RC (RMRP conditional) pups D. PCR analysis of pups derived from the interbreeding of RC mice and mice expressing CMV-Cre.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198473&req=5

pone-0026270-g001: Targeting of murine RMRP.A. Murine targeting vector (MTV) B. Southern blot of ES cells following selection for alleles with integrated MTV (the southern probe is shown in figure 1a) C. PCR analysis of RC (RMRP conditional) pups D. PCR analysis of pups derived from the interbreeding of RC mice and mice expressing CMV-Cre.
Mentions: We created a targeting vector specific for murine RMRP using the pEasyflox backbone [7]. The targeting vector contains the RMRP gene and promoter (800 bp up stream of murine RMRP [1]) flanked by two loxP sequences. A neomycin selectable marker flanked by two loxP sequences was placed upstream of RMRP (Figure 1A).

Bottom Line: RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia.To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional).We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, United States of America.

ABSTRACT
RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP mice, indicating that RMRP is essential for early embryonic development.

Show MeSH
Related in: MedlinePlus