Limits...
Geographically widespread swordfish barcode stock identification: a case study of its application.

Pappalardo AM, Guarino F, Reina S, Messina A, De Pinto V - PLoS ONE (2011)

Bottom Line: This information was successfully applied to the discrimination of unknown samples from the market, detecting in some cases mislabeled seafood products.Similar results were obtained with 5'dloop.Our preliminary data in swordfish Xiphias gladius confirm that Cytochrome Oxidase I can be proposed as an efficient species-specific marker that has also the potential to assign geographical provenance.

View Article: PubMed Central - PubMed

Affiliation: Section of Biochemistry and Molecular Biology, Department of Biological, Geological and Environmental Sciences, University of Catania, National Institute of Biomembranes and Biosystems, Section of Catania, Catania, Italy.

ABSTRACT

Background: The swordfish (Xiphias gladius) is a cosmopolitan large pelagic fish inhabiting tempered and tropical waters and it is a target species for fisheries all around the world. The present study investigated the ability of COI barcoding to reliably identify swordfish and particularly specific stocks of this commercially important species.

Methodology: We applied the classical DNA barcoding technology, upon a 682 bp segment of COI, and compared swordfish sequences from different geographical sources (Atlantic, Indian Oceans and Mediterranean Sea). The sequences of the 5' hyper-variable fragment of the control region (5'dloop), were also used to validate the efficacy of COI as a stock-specific marker.

Case report: This information was successfully applied to the discrimination of unknown samples from the market, detecting in some cases mislabeled seafood products.

Conclusions: The NJ distance-based phenogram (K2P model) obtained with COI sequences allowed us to correlate the swordfish haplotypes to the different geographical stocks. Similar results were obtained with 5'dloop. Our preliminary data in swordfish Xiphias gladius confirm that Cytochrome Oxidase I can be proposed as an efficient species-specific marker that has also the potential to assign geographical provenance. This information might speed the samples analysis in commercial application of barcoding.

Show MeSH
Frequency distribution of TACA repeats found in this study.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3198442&req=5

pone-0025516-g001: Frequency distribution of TACA repeats found in this study.

Mentions: One, two or three contiguous 5′-TACA-3′ sequence repeats were found at the 5′-end of the control region in the swordfish specimens examined in this work. TACA repeats were previously found in swordfish populations of the Atlantic Ocean and Mediterranean Sea [11], [1]. In this study, only the Mediterranean samples had a single 5′-TACA-3′ sequence. Samples from the Oceans and from the Mediterranean Sea had two 5′-TACA-3′ repeats, and, at the end, only one samples from the Mediterranean Sea and some from Atlantic Ocean had three 5′-TACA-3′ repeats. The proportion of samples with 2 repeats from the Atlantic Ocean and from the Mediterranean Sea was larger than that with 3 repeats (Fig. 1). Previous studies [11], [2] also indicated that no single repeat sample from swordfish was discovered in the Pacific Ocean, and only a very few swordfish carrying the single 5′-TACA-3′ sequence were also discovered off western Australian waters.


Geographically widespread swordfish barcode stock identification: a case study of its application.

Pappalardo AM, Guarino F, Reina S, Messina A, De Pinto V - PLoS ONE (2011)

Frequency distribution of TACA repeats found in this study.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198442&req=5

pone-0025516-g001: Frequency distribution of TACA repeats found in this study.
Mentions: One, two or three contiguous 5′-TACA-3′ sequence repeats were found at the 5′-end of the control region in the swordfish specimens examined in this work. TACA repeats were previously found in swordfish populations of the Atlantic Ocean and Mediterranean Sea [11], [1]. In this study, only the Mediterranean samples had a single 5′-TACA-3′ sequence. Samples from the Oceans and from the Mediterranean Sea had two 5′-TACA-3′ repeats, and, at the end, only one samples from the Mediterranean Sea and some from Atlantic Ocean had three 5′-TACA-3′ repeats. The proportion of samples with 2 repeats from the Atlantic Ocean and from the Mediterranean Sea was larger than that with 3 repeats (Fig. 1). Previous studies [11], [2] also indicated that no single repeat sample from swordfish was discovered in the Pacific Ocean, and only a very few swordfish carrying the single 5′-TACA-3′ sequence were also discovered off western Australian waters.

Bottom Line: This information was successfully applied to the discrimination of unknown samples from the market, detecting in some cases mislabeled seafood products.Similar results were obtained with 5'dloop.Our preliminary data in swordfish Xiphias gladius confirm that Cytochrome Oxidase I can be proposed as an efficient species-specific marker that has also the potential to assign geographical provenance.

View Article: PubMed Central - PubMed

Affiliation: Section of Biochemistry and Molecular Biology, Department of Biological, Geological and Environmental Sciences, University of Catania, National Institute of Biomembranes and Biosystems, Section of Catania, Catania, Italy.

ABSTRACT

Background: The swordfish (Xiphias gladius) is a cosmopolitan large pelagic fish inhabiting tempered and tropical waters and it is a target species for fisheries all around the world. The present study investigated the ability of COI barcoding to reliably identify swordfish and particularly specific stocks of this commercially important species.

Methodology: We applied the classical DNA barcoding technology, upon a 682 bp segment of COI, and compared swordfish sequences from different geographical sources (Atlantic, Indian Oceans and Mediterranean Sea). The sequences of the 5' hyper-variable fragment of the control region (5'dloop), were also used to validate the efficacy of COI as a stock-specific marker.

Case report: This information was successfully applied to the discrimination of unknown samples from the market, detecting in some cases mislabeled seafood products.

Conclusions: The NJ distance-based phenogram (K2P model) obtained with COI sequences allowed us to correlate the swordfish haplotypes to the different geographical stocks. Similar results were obtained with 5'dloop. Our preliminary data in swordfish Xiphias gladius confirm that Cytochrome Oxidase I can be proposed as an efficient species-specific marker that has also the potential to assign geographical provenance. This information might speed the samples analysis in commercial application of barcoding.

Show MeSH