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Presence of cartilage stem/progenitor cells in adult mice auricular perichondrium.

Kobayashi S, Takebe T, Zheng YW, Mizuno M, Yabuki Y, Maegawa J, Taniguchi H - PLoS ONE (2011)

Bottom Line: Furthermore, LRCs were successfully isolated and cultivated from auricular cartilage.Immunocytochemical analyses showed that LRCs express CD44 and integrin-α(5).Further characterization and utilization of the cell population should improve our understanding of basic cartilage biology and lead to advances in cartilage tissue engineering and novel therapeutic strategies for patients with craniofacial defects, including long-term tissue restoration.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Kanazawa-ku, Yokohama, Kanagawa, Japan.

ABSTRACT

Background: Based on evidence from several other tissues, cartilage stem/progenitor cells in the auricular cartilage presumably contribute to tissue development or homeostasis of the auricle. However, no definitive studies have identified or characterized a stem/progenitor population in mice auricle.

Methodology/principal findings: The 5-bromo-2'-deoxyuridine (BrdU) label-retaining technique was used to label dividing cells in fetal mice. Observations one year following the labeling revealed that label-retaining cells (LRCs) were present specifically in auricular perichondrium at a rate of 0.08±0.06%, but LRCs were not present in chondrium. Furthermore, LRCs were successfully isolated and cultivated from auricular cartilage. Immunocytochemical analyses showed that LRCs express CD44 and integrin-α(5). These LRCs, putative stem/progenitor cells, possess clonogenicity and chondrogenic capability in vitro.

Conclusions/significance: We have identified a population of putative cartilage stem/progenitor cells in the auricular perichondrium of mice. Further characterization and utilization of the cell population should improve our understanding of basic cartilage biology and lead to advances in cartilage tissue engineering and novel therapeutic strategies for patients with craniofacial defects, including long-term tissue restoration.

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Related in: MedlinePlus

Localization of long-term LRCs in different parts of the external ear.(A) A few LRCs were present in the perichondrium in the distal and middle areas of the external ear (White arrowheads). Many LRCs were present in the opening of the external acoustic meatus (White arrowheads). Two-headed arrows: Chondrium. Scale bar = 50 µm. Original magnification: ×40 (Alcian blue staining), ×100 (BrdU staining). (B) The LRCs of the distal and middle areas of the external ear and in the opening of the external acoustic meatus. The LRCs in the opening of the external acoustic meatus was much higher than that of the distal and middle areas (N = 6).
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pone-0026393-g004: Localization of long-term LRCs in different parts of the external ear.(A) A few LRCs were present in the perichondrium in the distal and middle areas of the external ear (White arrowheads). Many LRCs were present in the opening of the external acoustic meatus (White arrowheads). Two-headed arrows: Chondrium. Scale bar = 50 µm. Original magnification: ×40 (Alcian blue staining), ×100 (BrdU staining). (B) The LRCs of the distal and middle areas of the external ear and in the opening of the external acoustic meatus. The LRCs in the opening of the external acoustic meatus was much higher than that of the distal and middle areas (N = 6).

Mentions: To determine the distribution of LRCs in the auricular cartilage, the auricles of 24-week-old BrdU-labeled mice were analyzed (Figure 4A). Interestingly, the proximal part, i.e., the opening of the external acoustic meatus, contained a higher percentage of LRCs (23.87±0.07%) than the distal (0.02±0.03%) or middle (0.01±0.00%) parts of the auricle (N = 3) (Figure 4B).


Presence of cartilage stem/progenitor cells in adult mice auricular perichondrium.

Kobayashi S, Takebe T, Zheng YW, Mizuno M, Yabuki Y, Maegawa J, Taniguchi H - PLoS ONE (2011)

Localization of long-term LRCs in different parts of the external ear.(A) A few LRCs were present in the perichondrium in the distal and middle areas of the external ear (White arrowheads). Many LRCs were present in the opening of the external acoustic meatus (White arrowheads). Two-headed arrows: Chondrium. Scale bar = 50 µm. Original magnification: ×40 (Alcian blue staining), ×100 (BrdU staining). (B) The LRCs of the distal and middle areas of the external ear and in the opening of the external acoustic meatus. The LRCs in the opening of the external acoustic meatus was much higher than that of the distal and middle areas (N = 6).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198405&req=5

pone-0026393-g004: Localization of long-term LRCs in different parts of the external ear.(A) A few LRCs were present in the perichondrium in the distal and middle areas of the external ear (White arrowheads). Many LRCs were present in the opening of the external acoustic meatus (White arrowheads). Two-headed arrows: Chondrium. Scale bar = 50 µm. Original magnification: ×40 (Alcian blue staining), ×100 (BrdU staining). (B) The LRCs of the distal and middle areas of the external ear and in the opening of the external acoustic meatus. The LRCs in the opening of the external acoustic meatus was much higher than that of the distal and middle areas (N = 6).
Mentions: To determine the distribution of LRCs in the auricular cartilage, the auricles of 24-week-old BrdU-labeled mice were analyzed (Figure 4A). Interestingly, the proximal part, i.e., the opening of the external acoustic meatus, contained a higher percentage of LRCs (23.87±0.07%) than the distal (0.02±0.03%) or middle (0.01±0.00%) parts of the auricle (N = 3) (Figure 4B).

Bottom Line: Furthermore, LRCs were successfully isolated and cultivated from auricular cartilage.Immunocytochemical analyses showed that LRCs express CD44 and integrin-α(5).Further characterization and utilization of the cell population should improve our understanding of basic cartilage biology and lead to advances in cartilage tissue engineering and novel therapeutic strategies for patients with craniofacial defects, including long-term tissue restoration.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Kanazawa-ku, Yokohama, Kanagawa, Japan.

ABSTRACT

Background: Based on evidence from several other tissues, cartilage stem/progenitor cells in the auricular cartilage presumably contribute to tissue development or homeostasis of the auricle. However, no definitive studies have identified or characterized a stem/progenitor population in mice auricle.

Methodology/principal findings: The 5-bromo-2'-deoxyuridine (BrdU) label-retaining technique was used to label dividing cells in fetal mice. Observations one year following the labeling revealed that label-retaining cells (LRCs) were present specifically in auricular perichondrium at a rate of 0.08±0.06%, but LRCs were not present in chondrium. Furthermore, LRCs were successfully isolated and cultivated from auricular cartilage. Immunocytochemical analyses showed that LRCs express CD44 and integrin-α(5). These LRCs, putative stem/progenitor cells, possess clonogenicity and chondrogenic capability in vitro.

Conclusions/significance: We have identified a population of putative cartilage stem/progenitor cells in the auricular perichondrium of mice. Further characterization and utilization of the cell population should improve our understanding of basic cartilage biology and lead to advances in cartilage tissue engineering and novel therapeutic strategies for patients with craniofacial defects, including long-term tissue restoration.

Show MeSH
Related in: MedlinePlus