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Nasal chemosensory-stimulation evoked activity patterns in the rat trigeminal ganglion visualized by in vivo voltage-sensitive dye imaging.

Rothermel M, Ng BS, Grabska-Barwińska A, Hatt H, Jancke D - PLoS ONE (2011)

Bottom Line: Mammalian nasal chemosensation is predominantly mediated by two independent neuronal pathways, the olfactory and the trigeminal system.Within the early olfactory system, spatiotemporal responses of the olfactory bulb to various odorants have been mapped in great detail.Application of different chemical stimuli to the nasal cavity elicited distinct, stimulus-category specific, spatiotemporal activation patterns that comprised activated as well as suppressed areas.

View Article: PubMed Central - PubMed

Affiliation: Lehrstuhl für Zellphysiologie, Ruhr-Universität, Bochum, Germany.

ABSTRACT
Mammalian nasal chemosensation is predominantly mediated by two independent neuronal pathways, the olfactory and the trigeminal system. Within the early olfactory system, spatiotemporal responses of the olfactory bulb to various odorants have been mapped in great detail. In contrast, far less is known about the representation of volatile chemical stimuli at an early stage in the trigeminal system, the trigeminal ganglion (TG), which contains neurons directly projecting to the nasal cavity. We have established an in vivo preparation that allows high-resolution imaging of neuronal population activity from a large region of the rat TG using voltage-sensitive dyes (VSDs). Application of different chemical stimuli to the nasal cavity elicited distinct, stimulus-category specific, spatiotemporal activation patterns that comprised activated as well as suppressed areas. Thus, our results provide the first direct insights into the spatial representation of nasal chemosensory information within the trigeminal ganglion imaged at high temporal resolution.

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Local VSD activity correlates with suprathreshold electrical activity.(A) Single VSD trials obtained from the marked regions in the insert (left ethanol; right CO2). (B) Raster plots displaying single unit, single trial spiking activity obtained for the marked electrode penetration sites. Note that raster plots (B) and single trial VSD activity pattern (A) display similar patterns and latencies. Color scale = ΔF/F; red line = stimulus onset.
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pone-0026158-g004: Local VSD activity correlates with suprathreshold electrical activity.(A) Single VSD trials obtained from the marked regions in the insert (left ethanol; right CO2). (B) Raster plots displaying single unit, single trial spiking activity obtained for the marked electrode penetration sites. Note that raster plots (B) and single trial VSD activity pattern (A) display similar patterns and latencies. Color scale = ΔF/F; red line = stimulus onset.

Mentions: In order to characterize the evoked dynamics around responsive regions, activity was sampled across regions of interests guided by the layout of the evoked spatial patterns (see colored spots 1/2/3 in Figure 3A). Figure 3C shows the corresponding local time courses of activity (ΔF/F) at each of these regions (see Figure S4 for the relationship of each local timecourse to the “blank” (no stimulus) condition). Clear stimulation-dependent modulations were observed only in the ethanol condition, mainly in activated (Figure 3C, spot 1 and 2) but also in suppressed regions (spot 3). While ethanol evoked temporally irregular pulses of activation, evoked temporal modulations by CO2 as observed in single trials (Fig. 4A), were more regular in average traces (Fig. 3C, second column). Finally, citral and vanillin elicited uniform activation at all three regions without clear temporal modulations (Figure 3C, last two columns).


Nasal chemosensory-stimulation evoked activity patterns in the rat trigeminal ganglion visualized by in vivo voltage-sensitive dye imaging.

Rothermel M, Ng BS, Grabska-Barwińska A, Hatt H, Jancke D - PLoS ONE (2011)

Local VSD activity correlates with suprathreshold electrical activity.(A) Single VSD trials obtained from the marked regions in the insert (left ethanol; right CO2). (B) Raster plots displaying single unit, single trial spiking activity obtained for the marked electrode penetration sites. Note that raster plots (B) and single trial VSD activity pattern (A) display similar patterns and latencies. Color scale = ΔF/F; red line = stimulus onset.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198387&req=5

pone-0026158-g004: Local VSD activity correlates with suprathreshold electrical activity.(A) Single VSD trials obtained from the marked regions in the insert (left ethanol; right CO2). (B) Raster plots displaying single unit, single trial spiking activity obtained for the marked electrode penetration sites. Note that raster plots (B) and single trial VSD activity pattern (A) display similar patterns and latencies. Color scale = ΔF/F; red line = stimulus onset.
Mentions: In order to characterize the evoked dynamics around responsive regions, activity was sampled across regions of interests guided by the layout of the evoked spatial patterns (see colored spots 1/2/3 in Figure 3A). Figure 3C shows the corresponding local time courses of activity (ΔF/F) at each of these regions (see Figure S4 for the relationship of each local timecourse to the “blank” (no stimulus) condition). Clear stimulation-dependent modulations were observed only in the ethanol condition, mainly in activated (Figure 3C, spot 1 and 2) but also in suppressed regions (spot 3). While ethanol evoked temporally irregular pulses of activation, evoked temporal modulations by CO2 as observed in single trials (Fig. 4A), were more regular in average traces (Fig. 3C, second column). Finally, citral and vanillin elicited uniform activation at all three regions without clear temporal modulations (Figure 3C, last two columns).

Bottom Line: Mammalian nasal chemosensation is predominantly mediated by two independent neuronal pathways, the olfactory and the trigeminal system.Within the early olfactory system, spatiotemporal responses of the olfactory bulb to various odorants have been mapped in great detail.Application of different chemical stimuli to the nasal cavity elicited distinct, stimulus-category specific, spatiotemporal activation patterns that comprised activated as well as suppressed areas.

View Article: PubMed Central - PubMed

Affiliation: Lehrstuhl für Zellphysiologie, Ruhr-Universität, Bochum, Germany.

ABSTRACT
Mammalian nasal chemosensation is predominantly mediated by two independent neuronal pathways, the olfactory and the trigeminal system. Within the early olfactory system, spatiotemporal responses of the olfactory bulb to various odorants have been mapped in great detail. In contrast, far less is known about the representation of volatile chemical stimuli at an early stage in the trigeminal system, the trigeminal ganglion (TG), which contains neurons directly projecting to the nasal cavity. We have established an in vivo preparation that allows high-resolution imaging of neuronal population activity from a large region of the rat TG using voltage-sensitive dyes (VSDs). Application of different chemical stimuli to the nasal cavity elicited distinct, stimulus-category specific, spatiotemporal activation patterns that comprised activated as well as suppressed areas. Thus, our results provide the first direct insights into the spatial representation of nasal chemosensory information within the trigeminal ganglion imaged at high temporal resolution.

Show MeSH
Related in: MedlinePlus