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Genotypic and phenotypic properties of cattle-associated Campylobacter and their implications to public health in the USA.

Sanad YM, Kassem II, Abley M, Gebreyes W, LeJeune JT, Rajashekara G - PLoS ONE (2011)

Bottom Line: The prevalence of Campylobacter varied regionally and was significantly (P<0.05) higher in fecal samples collected from the South (32.8%) as compared to those from the North (14.8%), Midwest (15.83%), and East (12%).Furthermore, many cattle associated Campylobacter isolates showed resistance to several antimicrobials including ciprofloxacin, erythromycin, and gentamicin.Taken together, our results highlight the importance of cattle as a potential reservoir for clinically important Campylobacter.

View Article: PubMed Central - PubMed

Affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States of America.

ABSTRACT
Since cattle are a major source of food and the cattle industry engages people from farms to processing plants and meat markets, it is conceivable that beef-products contaminated with Campylobacter spp. would pose a significant public health concern. To better understand the epidemiology of cattle-associated Campylobacter spp. in the USA, we characterized the prevalence, genotypic and phenotypic properties of these pathogens. Campylobacter were detected in 181 (19.2%) out of 944 fecal samples. Specifically, 71 C. jejuni, 132 C. coli, and 10 other Campylobacter spp. were identified. The prevalence of Campylobacter varied regionally and was significantly (P<0.05) higher in fecal samples collected from the South (32.8%) as compared to those from the North (14.8%), Midwest (15.83%), and East (12%). Pulsed Field Gel Electrophoresis (PFGE) analysis showed that C. jejuni and C. coli isolates were genotypically diverse and certain genotypes were shared across two or more of the geographic locations. In addition, 13 new C. jejuni and two C. coli sequence types (STs) were detected by Multi Locus Sequence Typing (MLST). C. jejuni associated with clinically human health important sequence type, ST-61 which was not previously reported in the USA, was identified in the present study. Most frequently observed clonal complexes (CC) were CC ST-21, CC ST-42, and CC ST-61, which are also common in humans. Further, the cattle associated C. jejuni strains showed varying invasion and intracellular survival capacity; however, C. coli strains showed a lower invasion and intracellular survival potential compared to C. jejuni strains. Furthermore, many cattle associated Campylobacter isolates showed resistance to several antimicrobials including ciprofloxacin, erythromycin, and gentamicin. Taken together, our results highlight the importance of cattle as a potential reservoir for clinically important Campylobacter.

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Related in: MedlinePlus

Invasion and intracellular survival of cattle C. coli isolates in INT407 cells.A. CFU ml−1 representing the number of the internalized bacteria which could be retrieved after treatment of cells with gentamicin. B. Intracellular survival of C. coli isolates in INT407cells. CFU ml−1 representing the numbers of internalized bacteria retrieved after 24 h of incubation following the gentamicin treatment. The INT407 cells were infected with 1∶100 MOI of C. coli strains. C. coli (ATCC 33559) and C. jejuni NCTC11168 were used as controls. The detection limit of the assay is represented by the dashed line. Each bar represents the mean ± SE of three independent experiments done in duplicates for each sample (P<0.01).
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pone-0025778-g004: Invasion and intracellular survival of cattle C. coli isolates in INT407 cells.A. CFU ml−1 representing the number of the internalized bacteria which could be retrieved after treatment of cells with gentamicin. B. Intracellular survival of C. coli isolates in INT407cells. CFU ml−1 representing the numbers of internalized bacteria retrieved after 24 h of incubation following the gentamicin treatment. The INT407 cells were infected with 1∶100 MOI of C. coli strains. C. coli (ATCC 33559) and C. jejuni NCTC11168 were used as controls. The detection limit of the assay is represented by the dashed line. Each bar represents the mean ± SE of three independent experiments done in duplicates for each sample (P<0.01).

Mentions: On the other hand, only nine C. coli isolates could invade the INT407 cells with average numbers ranging between 2.75×101 and 1.34×104 CFU ml−1 (Fig. 4A). There was a significant difference in the invasion capabilities between the C.coli isolates (P<0.05). Furthermore, only seven isolates were capable of intracellular survival (Fig. 4B). Three isolates that did not invade were not tested for intracellular survival. In general C. coli isolates were less invasive and displayed reduced intracellular survival compared to C. jejuni.


Genotypic and phenotypic properties of cattle-associated Campylobacter and their implications to public health in the USA.

Sanad YM, Kassem II, Abley M, Gebreyes W, LeJeune JT, Rajashekara G - PLoS ONE (2011)

Invasion and intracellular survival of cattle C. coli isolates in INT407 cells.A. CFU ml−1 representing the number of the internalized bacteria which could be retrieved after treatment of cells with gentamicin. B. Intracellular survival of C. coli isolates in INT407cells. CFU ml−1 representing the numbers of internalized bacteria retrieved after 24 h of incubation following the gentamicin treatment. The INT407 cells were infected with 1∶100 MOI of C. coli strains. C. coli (ATCC 33559) and C. jejuni NCTC11168 were used as controls. The detection limit of the assay is represented by the dashed line. Each bar represents the mean ± SE of three independent experiments done in duplicates for each sample (P<0.01).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198382&req=5

pone-0025778-g004: Invasion and intracellular survival of cattle C. coli isolates in INT407 cells.A. CFU ml−1 representing the number of the internalized bacteria which could be retrieved after treatment of cells with gentamicin. B. Intracellular survival of C. coli isolates in INT407cells. CFU ml−1 representing the numbers of internalized bacteria retrieved after 24 h of incubation following the gentamicin treatment. The INT407 cells were infected with 1∶100 MOI of C. coli strains. C. coli (ATCC 33559) and C. jejuni NCTC11168 were used as controls. The detection limit of the assay is represented by the dashed line. Each bar represents the mean ± SE of three independent experiments done in duplicates for each sample (P<0.01).
Mentions: On the other hand, only nine C. coli isolates could invade the INT407 cells with average numbers ranging between 2.75×101 and 1.34×104 CFU ml−1 (Fig. 4A). There was a significant difference in the invasion capabilities between the C.coli isolates (P<0.05). Furthermore, only seven isolates were capable of intracellular survival (Fig. 4B). Three isolates that did not invade were not tested for intracellular survival. In general C. coli isolates were less invasive and displayed reduced intracellular survival compared to C. jejuni.

Bottom Line: The prevalence of Campylobacter varied regionally and was significantly (P<0.05) higher in fecal samples collected from the South (32.8%) as compared to those from the North (14.8%), Midwest (15.83%), and East (12%).Furthermore, many cattle associated Campylobacter isolates showed resistance to several antimicrobials including ciprofloxacin, erythromycin, and gentamicin.Taken together, our results highlight the importance of cattle as a potential reservoir for clinically important Campylobacter.

View Article: PubMed Central - PubMed

Affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States of America.

ABSTRACT
Since cattle are a major source of food and the cattle industry engages people from farms to processing plants and meat markets, it is conceivable that beef-products contaminated with Campylobacter spp. would pose a significant public health concern. To better understand the epidemiology of cattle-associated Campylobacter spp. in the USA, we characterized the prevalence, genotypic and phenotypic properties of these pathogens. Campylobacter were detected in 181 (19.2%) out of 944 fecal samples. Specifically, 71 C. jejuni, 132 C. coli, and 10 other Campylobacter spp. were identified. The prevalence of Campylobacter varied regionally and was significantly (P<0.05) higher in fecal samples collected from the South (32.8%) as compared to those from the North (14.8%), Midwest (15.83%), and East (12%). Pulsed Field Gel Electrophoresis (PFGE) analysis showed that C. jejuni and C. coli isolates were genotypically diverse and certain genotypes were shared across two or more of the geographic locations. In addition, 13 new C. jejuni and two C. coli sequence types (STs) were detected by Multi Locus Sequence Typing (MLST). C. jejuni associated with clinically human health important sequence type, ST-61 which was not previously reported in the USA, was identified in the present study. Most frequently observed clonal complexes (CC) were CC ST-21, CC ST-42, and CC ST-61, which are also common in humans. Further, the cattle associated C. jejuni strains showed varying invasion and intracellular survival capacity; however, C. coli strains showed a lower invasion and intracellular survival potential compared to C. jejuni strains. Furthermore, many cattle associated Campylobacter isolates showed resistance to several antimicrobials including ciprofloxacin, erythromycin, and gentamicin. Taken together, our results highlight the importance of cattle as a potential reservoir for clinically important Campylobacter.

Show MeSH
Related in: MedlinePlus