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Clinical significance of vasculogenic mimicry in human gliomas.

Liu XM, Zhang QP, Mu YG, Zhang XH, Sai K, Pang JC, Ng HK, Chen ZP - J. Neurooncol. (2011)

Bottom Line: We have previously found the presence of VM in high-grade gliomas.Vasculogenic mimicry channels were associated with the expression of COX-2 and MMP-9 (P < 0.05).Our results suggest that VM channels in gliomas correlate with increasing malignancy and higher aggressiveness, and may provide a complementation to the tumor's blood supply, especially in less vascularized regions, which may aid in the identification of glioma patients with a poorer prognosis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oncology in South China and Department of Neurosurgery/Neuro-Oncology, Cancer Center, Sun Yat-sen University, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China.

ABSTRACT
Vasculogenic mimicry (VM) is known as non-endothelial tumor cell-lined microvascular channels in aggressive tumors. We have previously found the presence of VM in high-grade gliomas. In this study, we aimed to identify VM patterns in gliomas and to explore their clinical significance. Tumor samples as well as their detailed clinical/prognostic data were collected from 101 patients. Vasculogenic mimicry in the glioma samples was determined by dual staining for endothelial marker CD34 and periodic acid-Schiff (PAS). Tumor samples were also immunohistochemically stained for Ki-67, VEGF, COX-2 and MMP-9. The association between VM and the clinical characteristics of the patients were analyzed. A Kaplan-Meier survival analysis and log-rank tests were performed to compare survival times of the patients. Vasculogenic mimicry was present in 13 out of 101 samples. The higher grade gliomas had a higher incidence of VM than that of lower grade gliomas (P = 0.006). Vasculogenic mimicry channels were associated with the expression of COX-2 and MMP-9 (P < 0.05). While there was no association between the existence of VM and the sex, age and preoperative epilepsy of the patients, or expression of Ki-67 and VEGF. However, patients with VM-positive gliomas survived a shorter period of time than those with VM negative gliomas (P = 0.027). Interestingly, in high-grade gliomas, the level of microvascular density was lower in VM positive tumors than those VM negative tumors (P = 0.039). Our results suggest that VM channels in gliomas correlate with increasing malignancy and higher aggressiveness, and may provide a complementation to the tumor's blood supply, especially in less vascularized regions, which may aid in the identification of glioma patients with a poorer prognosis.

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Representative micrographs of VM in glioma. a PAS-positive (purple-red) zone without CD34 positive cells indicate VM; some angiogenesis vessels are seen with CD34 stained (brown) cells in the internal lumen of vessels. b A magnification of image (a); erythrocyte shadows are seen in the channels. c The cells composing the channels were negative for CD34 indicating that they were not endothelial or endothelial progenitor cells (VM zone); and CD34-positive and PAS-positive channels are seen in the same view (endothelial vessels). d Cells around the channels are stained positive for GFAP indicating that they are astrocytic tumor cells. Scale bars 50 μm. (Color figure online)
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Fig1: Representative micrographs of VM in glioma. a PAS-positive (purple-red) zone without CD34 positive cells indicate VM; some angiogenesis vessels are seen with CD34 stained (brown) cells in the internal lumen of vessels. b A magnification of image (a); erythrocyte shadows are seen in the channels. c The cells composing the channels were negative for CD34 indicating that they were not endothelial or endothelial progenitor cells (VM zone); and CD34-positive and PAS-positive channels are seen in the same view (endothelial vessels). d Cells around the channels are stained positive for GFAP indicating that they are astrocytic tumor cells. Scale bars 50 μm. (Color figure online)

Mentions: Although a PAS-positive pattern was seen in most of the samples, CD34-negative and PAS-positive channels, which were considered as VM, were found in 13 of 101 cases (12.9%) examined (Fig. 1). The cells composing the channels were negative for CD34 indicating that they were not endothelial or endothelial progenitor cells. The detection of GFAP expression in cells around the VM channels confirmed the cells as astrocytic tumor cells (Fig. 1).Fig. 1


Clinical significance of vasculogenic mimicry in human gliomas.

Liu XM, Zhang QP, Mu YG, Zhang XH, Sai K, Pang JC, Ng HK, Chen ZP - J. Neurooncol. (2011)

Representative micrographs of VM in glioma. a PAS-positive (purple-red) zone without CD34 positive cells indicate VM; some angiogenesis vessels are seen with CD34 stained (brown) cells in the internal lumen of vessels. b A magnification of image (a); erythrocyte shadows are seen in the channels. c The cells composing the channels were negative for CD34 indicating that they were not endothelial or endothelial progenitor cells (VM zone); and CD34-positive and PAS-positive channels are seen in the same view (endothelial vessels). d Cells around the channels are stained positive for GFAP indicating that they are astrocytic tumor cells. Scale bars 50 μm. (Color figure online)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3198193&req=5

Fig1: Representative micrographs of VM in glioma. a PAS-positive (purple-red) zone without CD34 positive cells indicate VM; some angiogenesis vessels are seen with CD34 stained (brown) cells in the internal lumen of vessels. b A magnification of image (a); erythrocyte shadows are seen in the channels. c The cells composing the channels were negative for CD34 indicating that they were not endothelial or endothelial progenitor cells (VM zone); and CD34-positive and PAS-positive channels are seen in the same view (endothelial vessels). d Cells around the channels are stained positive for GFAP indicating that they are astrocytic tumor cells. Scale bars 50 μm. (Color figure online)
Mentions: Although a PAS-positive pattern was seen in most of the samples, CD34-negative and PAS-positive channels, which were considered as VM, were found in 13 of 101 cases (12.9%) examined (Fig. 1). The cells composing the channels were negative for CD34 indicating that they were not endothelial or endothelial progenitor cells. The detection of GFAP expression in cells around the VM channels confirmed the cells as astrocytic tumor cells (Fig. 1).Fig. 1

Bottom Line: We have previously found the presence of VM in high-grade gliomas.Vasculogenic mimicry channels were associated with the expression of COX-2 and MMP-9 (P < 0.05).Our results suggest that VM channels in gliomas correlate with increasing malignancy and higher aggressiveness, and may provide a complementation to the tumor's blood supply, especially in less vascularized regions, which may aid in the identification of glioma patients with a poorer prognosis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oncology in South China and Department of Neurosurgery/Neuro-Oncology, Cancer Center, Sun Yat-sen University, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China.

ABSTRACT
Vasculogenic mimicry (VM) is known as non-endothelial tumor cell-lined microvascular channels in aggressive tumors. We have previously found the presence of VM in high-grade gliomas. In this study, we aimed to identify VM patterns in gliomas and to explore their clinical significance. Tumor samples as well as their detailed clinical/prognostic data were collected from 101 patients. Vasculogenic mimicry in the glioma samples was determined by dual staining for endothelial marker CD34 and periodic acid-Schiff (PAS). Tumor samples were also immunohistochemically stained for Ki-67, VEGF, COX-2 and MMP-9. The association between VM and the clinical characteristics of the patients were analyzed. A Kaplan-Meier survival analysis and log-rank tests were performed to compare survival times of the patients. Vasculogenic mimicry was present in 13 out of 101 samples. The higher grade gliomas had a higher incidence of VM than that of lower grade gliomas (P = 0.006). Vasculogenic mimicry channels were associated with the expression of COX-2 and MMP-9 (P < 0.05). While there was no association between the existence of VM and the sex, age and preoperative epilepsy of the patients, or expression of Ki-67 and VEGF. However, patients with VM-positive gliomas survived a shorter period of time than those with VM negative gliomas (P = 0.027). Interestingly, in high-grade gliomas, the level of microvascular density was lower in VM positive tumors than those VM negative tumors (P = 0.039). Our results suggest that VM channels in gliomas correlate with increasing malignancy and higher aggressiveness, and may provide a complementation to the tumor's blood supply, especially in less vascularized regions, which may aid in the identification of glioma patients with a poorer prognosis.

Show MeSH
Related in: MedlinePlus