Limits...
Prenatal VPA Exposure and Changes in Sensory Processing by the Superior Colliculus.

Dendrinos G, Hemelt M, Keller A - Front Integr Neurosci (2011)

Bottom Line: Disorders involving dysfunctional sensory processing are characterized by an inability to filter sensory information, particularly simultaneously arriving multimodal inputs.Some deficits reversed with age.These results suggest that prenatal VPA treatment affects the development of the superior colliculus and leads to persistent anatomical changes evidenced by aberrant behavior in tasks that require sensory processing.

View Article: PubMed Central - PubMed

Affiliation: Program in Neuroscience, Department of Anatomy and Neurobiology, University of Maryland School of Medicine Baltimore, MD, USA.

ABSTRACT
Disorders involving dysfunctional sensory processing are characterized by an inability to filter sensory information, particularly simultaneously arriving multimodal inputs. We examined the effects of prenatal exposure to valproic acid (VPA), a teratogen linked to sensory dysfunction, on the behavior of juvenile and adult rats, and on the anatomy of the superior colliculus, a critical multisensory integration center in the brain. VPA-exposed rats showed deficits in colliculus-dependent behaviors including startle response, prepulse inhibition, and nociceptive responses. Some deficits reversed with age. Stereological analyses revealed that colliculi of VPA-treated rats had significantly fewer parvalbumin-positive neurons, a subset of GABAergic cells. These results suggest that prenatal VPA treatment affects the development of the superior colliculus and leads to persistent anatomical changes evidenced by aberrant behavior in tasks that require sensory processing.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence double staining for parvalbumin [green, (A)], GABA [red, (B)], and their colocalization [yellow, (C)]. 95% of neurons expressing parvalbumin also express GABA. Arrow in (C) denotes a GABAergic neuron that is not parvalbumin-positive. Scale bar = 50 μm. (D–G). Effects of prenatal VPA treatment on cell numbers in the superior colliculus of adult rats. (D,E). Total number of Nissl-stained cells and total number of neurons labeled with NeuN in the superficial (D) and the intermediate/deep (E) layers. (F,G). VPA treatment results in significant reductions in the number of parvalbumin-positive neurons in the superficial (F) and the intermediate/deep (G) layers. Mann–Whitney U used for statistical analyses. Sample size is denoted by number on each bar.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3198155&req=5

Figure 5: Immunofluorescence double staining for parvalbumin [green, (A)], GABA [red, (B)], and their colocalization [yellow, (C)]. 95% of neurons expressing parvalbumin also express GABA. Arrow in (C) denotes a GABAergic neuron that is not parvalbumin-positive. Scale bar = 50 μm. (D–G). Effects of prenatal VPA treatment on cell numbers in the superior colliculus of adult rats. (D,E). Total number of Nissl-stained cells and total number of neurons labeled with NeuN in the superficial (D) and the intermediate/deep (E) layers. (F,G). VPA treatment results in significant reductions in the number of parvalbumin-positive neurons in the superficial (F) and the intermediate/deep (G) layers. Mann–Whitney U used for statistical analyses. Sample size is denoted by number on each bar.

Mentions: All anatomical experiments were carried out on adult (PND >60) offspring of animals that received, on E12.5, a single 600 mg/kg i.p. injection of VPA or saline. See Figure 5 for sample size for each stereological analysis.


Prenatal VPA Exposure and Changes in Sensory Processing by the Superior Colliculus.

Dendrinos G, Hemelt M, Keller A - Front Integr Neurosci (2011)

Immunofluorescence double staining for parvalbumin [green, (A)], GABA [red, (B)], and their colocalization [yellow, (C)]. 95% of neurons expressing parvalbumin also express GABA. Arrow in (C) denotes a GABAergic neuron that is not parvalbumin-positive. Scale bar = 50 μm. (D–G). Effects of prenatal VPA treatment on cell numbers in the superior colliculus of adult rats. (D,E). Total number of Nissl-stained cells and total number of neurons labeled with NeuN in the superficial (D) and the intermediate/deep (E) layers. (F,G). VPA treatment results in significant reductions in the number of parvalbumin-positive neurons in the superficial (F) and the intermediate/deep (G) layers. Mann–Whitney U used for statistical analyses. Sample size is denoted by number on each bar.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3198155&req=5

Figure 5: Immunofluorescence double staining for parvalbumin [green, (A)], GABA [red, (B)], and their colocalization [yellow, (C)]. 95% of neurons expressing parvalbumin also express GABA. Arrow in (C) denotes a GABAergic neuron that is not parvalbumin-positive. Scale bar = 50 μm. (D–G). Effects of prenatal VPA treatment on cell numbers in the superior colliculus of adult rats. (D,E). Total number of Nissl-stained cells and total number of neurons labeled with NeuN in the superficial (D) and the intermediate/deep (E) layers. (F,G). VPA treatment results in significant reductions in the number of parvalbumin-positive neurons in the superficial (F) and the intermediate/deep (G) layers. Mann–Whitney U used for statistical analyses. Sample size is denoted by number on each bar.
Mentions: All anatomical experiments were carried out on adult (PND >60) offspring of animals that received, on E12.5, a single 600 mg/kg i.p. injection of VPA or saline. See Figure 5 for sample size for each stereological analysis.

Bottom Line: Disorders involving dysfunctional sensory processing are characterized by an inability to filter sensory information, particularly simultaneously arriving multimodal inputs.Some deficits reversed with age.These results suggest that prenatal VPA treatment affects the development of the superior colliculus and leads to persistent anatomical changes evidenced by aberrant behavior in tasks that require sensory processing.

View Article: PubMed Central - PubMed

Affiliation: Program in Neuroscience, Department of Anatomy and Neurobiology, University of Maryland School of Medicine Baltimore, MD, USA.

ABSTRACT
Disorders involving dysfunctional sensory processing are characterized by an inability to filter sensory information, particularly simultaneously arriving multimodal inputs. We examined the effects of prenatal exposure to valproic acid (VPA), a teratogen linked to sensory dysfunction, on the behavior of juvenile and adult rats, and on the anatomy of the superior colliculus, a critical multisensory integration center in the brain. VPA-exposed rats showed deficits in colliculus-dependent behaviors including startle response, prepulse inhibition, and nociceptive responses. Some deficits reversed with age. Stereological analyses revealed that colliculi of VPA-treated rats had significantly fewer parvalbumin-positive neurons, a subset of GABAergic cells. These results suggest that prenatal VPA treatment affects the development of the superior colliculus and leads to persistent anatomical changes evidenced by aberrant behavior in tasks that require sensory processing.

No MeSH data available.


Related in: MedlinePlus