Limits...
The relationship between structurally different pyrrolizidine alkaloids and western flower thrips resistance in F(2) hybrids of Jacobaea vulgaris and Jacobaea aquatica.

Cheng D, Kirk H, Vrieling K, Mulder PP, Klinkhamer PG - J. Chem. Ecol. (2011)

Bottom Line: Among 37 individual PAs that were derived from four structural groups (senecionine-, jacobine-, erucifoline- and otosenine-like PAs), the N-oxides of jacobine, jaconine, and jacoline were negatively correlated with feeding damage caused by WFT, and the tertiary amines of jacobine, jaconine, jacoline, and other PAs did not relate to feeding damage.Multiple regression tests suggested that jacobine-like PAs play a greater role in WFT resistance than PAs from other structural groups.We found no evidence for synergistic effects of different PAs on WFT resistance.

View Article: PubMed Central - PubMed

Affiliation: Plant Ecology & Phytochemistry, Institute of Biology, Leiden University, the Netherlands. d.d.cheng@biology.leidenuniv.nl

ABSTRACT
Segregating plant hybrids often have more ecological and molecular variability compared to parental species, and are therefore useful for studying relationships between different traits, and the adaptive significance of trait variation. Hybrid systems have been used to study the relationship between the expression of plant defense compounds and herbivore susceptibility. We conducted a western flower thrips (WFT) bioassay using a hybrid family and investigated the relationship between WFT resistance and pyrrolizidine alkaloid (PA) variation. The hybrid family consisted of two parental (Jacobaea vulgaris and Jacobaea aquatica) genotypes, two F(1) genotypes, and 94 F(2) hybrid lines. The J. aquatica genotype was more susceptible to thrips attack than the J. vulgaris genotype, the two F(1) hybrids were as susceptible as J. aquatica, and susceptibility to WFT differed among F(2) hybrid lines: 69 F(2) lines were equally susceptible compared to J. aquatica, 10 F(2) lines were more susceptible than J. aquatica and 15 F(2) lines were as resistant as J. vulgaris or were intermediate to the two parental genotypes. Among 37 individual PAs that were derived from four structural groups (senecionine-, jacobine-, erucifoline- and otosenine-like PAs), the N-oxides of jacobine, jaconine, and jacoline were negatively correlated with feeding damage caused by WFT, and the tertiary amines of jacobine, jaconine, jacoline, and other PAs did not relate to feeding damage. Total PA concentration was negatively correlated with feeding damage. Among the four PA groups, only the total concentration of the jacobine-like PAs was negatively correlated with feeding damage. Multiple regression tests suggested that jacobine-like PAs play a greater role in WFT resistance than PAs from other structural groups. We found no evidence for synergistic effects of different PAs on WFT resistance. The relationship between PA variation and WFT feeding damage in the Jacobaea hybrids suggests a role for PAs in resistance to generalist insects.

Show MeSH

Related in: MedlinePlus

Relationship between feeding damage by western flower thrips (WFT) (mm2) and the concentration of total pyrrolizidine alkaloid (PA), senecionine-like, jacobine-like, and erucifoline-like PAs (μg/g dw) of F2 hybrids of Jacobaea aquatica and Jacobaea vulgaris. The data for WFT feeding damage and concentrations are the log-transformed genotypic mean values. In each panel, the results of the Pearson correlation tests between feeding damage and the PA concentrations are provided; in all cases, d.f. = 92
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3197926&req=5

Fig2: Relationship between feeding damage by western flower thrips (WFT) (mm2) and the concentration of total pyrrolizidine alkaloid (PA), senecionine-like, jacobine-like, and erucifoline-like PAs (μg/g dw) of F2 hybrids of Jacobaea aquatica and Jacobaea vulgaris. The data for WFT feeding damage and concentrations are the log-transformed genotypic mean values. In each panel, the results of the Pearson correlation tests between feeding damage and the PA concentrations are provided; in all cases, d.f. = 92

Mentions: Relationship Between Feeding Damage and PA Concentration Correlation tests between feeding damage and individual PAs showed that feeding damage was negatively correlated with the concentrations of the N-oxides of two jacobine-like PAs (jaconine and jacoline). Jacobine N-oxide concentration was marginally correlated with feeding damage, and the correlations between the free bases of jacobine-like PAs and feeding damage were not significant after correction for multiple testing. No other individual PAs were correlated with feeding damage (Table 2). Total PA concentration also was correlated with feeding damage (Fig. 2a). Of the four structural groups of PAs, only the sum concentration of jacobine-like PAs was significantly correlated with feeding damage by WFT (Fig. 2c). The sum concentrations of the other three groups were not correlated with feeding damage (see the statistical results for senecionine-and erucifoline-like PAs in Fig. 2b,d; for otosenine-like PAs: d.f. = 92, r = 0.35, P = 0.77).Fig. 2


The relationship between structurally different pyrrolizidine alkaloids and western flower thrips resistance in F(2) hybrids of Jacobaea vulgaris and Jacobaea aquatica.

Cheng D, Kirk H, Vrieling K, Mulder PP, Klinkhamer PG - J. Chem. Ecol. (2011)

Relationship between feeding damage by western flower thrips (WFT) (mm2) and the concentration of total pyrrolizidine alkaloid (PA), senecionine-like, jacobine-like, and erucifoline-like PAs (μg/g dw) of F2 hybrids of Jacobaea aquatica and Jacobaea vulgaris. The data for WFT feeding damage and concentrations are the log-transformed genotypic mean values. In each panel, the results of the Pearson correlation tests between feeding damage and the PA concentrations are provided; in all cases, d.f. = 92
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3197926&req=5

Fig2: Relationship between feeding damage by western flower thrips (WFT) (mm2) and the concentration of total pyrrolizidine alkaloid (PA), senecionine-like, jacobine-like, and erucifoline-like PAs (μg/g dw) of F2 hybrids of Jacobaea aquatica and Jacobaea vulgaris. The data for WFT feeding damage and concentrations are the log-transformed genotypic mean values. In each panel, the results of the Pearson correlation tests between feeding damage and the PA concentrations are provided; in all cases, d.f. = 92
Mentions: Relationship Between Feeding Damage and PA Concentration Correlation tests between feeding damage and individual PAs showed that feeding damage was negatively correlated with the concentrations of the N-oxides of two jacobine-like PAs (jaconine and jacoline). Jacobine N-oxide concentration was marginally correlated with feeding damage, and the correlations between the free bases of jacobine-like PAs and feeding damage were not significant after correction for multiple testing. No other individual PAs were correlated with feeding damage (Table 2). Total PA concentration also was correlated with feeding damage (Fig. 2a). Of the four structural groups of PAs, only the sum concentration of jacobine-like PAs was significantly correlated with feeding damage by WFT (Fig. 2c). The sum concentrations of the other three groups were not correlated with feeding damage (see the statistical results for senecionine-and erucifoline-like PAs in Fig. 2b,d; for otosenine-like PAs: d.f. = 92, r = 0.35, P = 0.77).Fig. 2

Bottom Line: Among 37 individual PAs that were derived from four structural groups (senecionine-, jacobine-, erucifoline- and otosenine-like PAs), the N-oxides of jacobine, jaconine, and jacoline were negatively correlated with feeding damage caused by WFT, and the tertiary amines of jacobine, jaconine, jacoline, and other PAs did not relate to feeding damage.Multiple regression tests suggested that jacobine-like PAs play a greater role in WFT resistance than PAs from other structural groups.We found no evidence for synergistic effects of different PAs on WFT resistance.

View Article: PubMed Central - PubMed

Affiliation: Plant Ecology & Phytochemistry, Institute of Biology, Leiden University, the Netherlands. d.d.cheng@biology.leidenuniv.nl

ABSTRACT
Segregating plant hybrids often have more ecological and molecular variability compared to parental species, and are therefore useful for studying relationships between different traits, and the adaptive significance of trait variation. Hybrid systems have been used to study the relationship between the expression of plant defense compounds and herbivore susceptibility. We conducted a western flower thrips (WFT) bioassay using a hybrid family and investigated the relationship between WFT resistance and pyrrolizidine alkaloid (PA) variation. The hybrid family consisted of two parental (Jacobaea vulgaris and Jacobaea aquatica) genotypes, two F(1) genotypes, and 94 F(2) hybrid lines. The J. aquatica genotype was more susceptible to thrips attack than the J. vulgaris genotype, the two F(1) hybrids were as susceptible as J. aquatica, and susceptibility to WFT differed among F(2) hybrid lines: 69 F(2) lines were equally susceptible compared to J. aquatica, 10 F(2) lines were more susceptible than J. aquatica and 15 F(2) lines were as resistant as J. vulgaris or were intermediate to the two parental genotypes. Among 37 individual PAs that were derived from four structural groups (senecionine-, jacobine-, erucifoline- and otosenine-like PAs), the N-oxides of jacobine, jaconine, and jacoline were negatively correlated with feeding damage caused by WFT, and the tertiary amines of jacobine, jaconine, jacoline, and other PAs did not relate to feeding damage. Total PA concentration was negatively correlated with feeding damage. Among the four PA groups, only the total concentration of the jacobine-like PAs was negatively correlated with feeding damage. Multiple regression tests suggested that jacobine-like PAs play a greater role in WFT resistance than PAs from other structural groups. We found no evidence for synergistic effects of different PAs on WFT resistance. The relationship between PA variation and WFT feeding damage in the Jacobaea hybrids suggests a role for PAs in resistance to generalist insects.

Show MeSH
Related in: MedlinePlus