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The relationship between structurally different pyrrolizidine alkaloids and western flower thrips resistance in F(2) hybrids of Jacobaea vulgaris and Jacobaea aquatica.

Cheng D, Kirk H, Vrieling K, Mulder PP, Klinkhamer PG - J. Chem. Ecol. (2011)

Bottom Line: Among 37 individual PAs that were derived from four structural groups (senecionine-, jacobine-, erucifoline- and otosenine-like PAs), the N-oxides of jacobine, jaconine, and jacoline were negatively correlated with feeding damage caused by WFT, and the tertiary amines of jacobine, jaconine, jacoline, and other PAs did not relate to feeding damage.Multiple regression tests suggested that jacobine-like PAs play a greater role in WFT resistance than PAs from other structural groups.We found no evidence for synergistic effects of different PAs on WFT resistance.

View Article: PubMed Central - PubMed

Affiliation: Plant Ecology & Phytochemistry, Institute of Biology, Leiden University, the Netherlands. d.d.cheng@biology.leidenuniv.nl

ABSTRACT
Segregating plant hybrids often have more ecological and molecular variability compared to parental species, and are therefore useful for studying relationships between different traits, and the adaptive significance of trait variation. Hybrid systems have been used to study the relationship between the expression of plant defense compounds and herbivore susceptibility. We conducted a western flower thrips (WFT) bioassay using a hybrid family and investigated the relationship between WFT resistance and pyrrolizidine alkaloid (PA) variation. The hybrid family consisted of two parental (Jacobaea vulgaris and Jacobaea aquatica) genotypes, two F(1) genotypes, and 94 F(2) hybrid lines. The J. aquatica genotype was more susceptible to thrips attack than the J. vulgaris genotype, the two F(1) hybrids were as susceptible as J. aquatica, and susceptibility to WFT differed among F(2) hybrid lines: 69 F(2) lines were equally susceptible compared to J. aquatica, 10 F(2) lines were more susceptible than J. aquatica and 15 F(2) lines were as resistant as J. vulgaris or were intermediate to the two parental genotypes. Among 37 individual PAs that were derived from four structural groups (senecionine-, jacobine-, erucifoline- and otosenine-like PAs), the N-oxides of jacobine, jaconine, and jacoline were negatively correlated with feeding damage caused by WFT, and the tertiary amines of jacobine, jaconine, jacoline, and other PAs did not relate to feeding damage. Total PA concentration was negatively correlated with feeding damage. Among the four PA groups, only the total concentration of the jacobine-like PAs was negatively correlated with feeding damage. Multiple regression tests suggested that jacobine-like PAs play a greater role in WFT resistance than PAs from other structural groups. We found no evidence for synergistic effects of different PAs on WFT resistance. The relationship between PA variation and WFT feeding damage in the Jacobaea hybrids suggests a role for PAs in resistance to generalist insects.

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Variation in western flower thrips (WFT) feeding damage (mm2) on Jacobaea aquatica, Jacobaea vulgaris, 2 F1 and 94 F2 hybrids. a Mean feeding damage for one J. aquatica genotype (JA), one J. vulgaris genotype (JV), and 2 F1 (F1-A and F1 -B) genotypes. Error bars are standard errors, N = 12. J. vulgaris was significantly different from the other genotypes at * P < 0.05. b Distribution frequency for genotypic mean WFT feeding damage of 94 F2 hybrids. N = 3–6 for each genotype. In total, 587 plants were used in WFT bioassay
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Fig1: Variation in western flower thrips (WFT) feeding damage (mm2) on Jacobaea aquatica, Jacobaea vulgaris, 2 F1 and 94 F2 hybrids. a Mean feeding damage for one J. aquatica genotype (JA), one J. vulgaris genotype (JV), and 2 F1 (F1-A and F1 -B) genotypes. Error bars are standard errors, N = 12. J. vulgaris was significantly different from the other genotypes at * P < 0.05. b Distribution frequency for genotypic mean WFT feeding damage of 94 F2 hybrids. N = 3–6 for each genotype. In total, 587 plants were used in WFT bioassay

Mentions: Variation in Feeding Damage Feeding damage was genotype-dependent (d.f. = 97,488; F = 5.30; P < 0.001). Plant mass also had effect on feeding damage (d.f. = 1,488; F = 18.44; P < 0.001). Among the two parental genotypes, J. aquatica suffered more feeding damage than J. vulgaris (d.f. = 1, 22; t = 6.18; P < 0.001). Both of the F1 lines were as susceptible as J. aquatica. Among the 94 F2 hybrids, 69 were as susceptible as J. aquatica, 10 were more susceptible than J. aquatica, 15 showed intermediate resistance, 9 were as resistant as J. vulgaris, and none were more resistant than J. vulgaris (Fig. 1, see statistical details in Supplementary Table 1).Fig. 1


The relationship between structurally different pyrrolizidine alkaloids and western flower thrips resistance in F(2) hybrids of Jacobaea vulgaris and Jacobaea aquatica.

Cheng D, Kirk H, Vrieling K, Mulder PP, Klinkhamer PG - J. Chem. Ecol. (2011)

Variation in western flower thrips (WFT) feeding damage (mm2) on Jacobaea aquatica, Jacobaea vulgaris, 2 F1 and 94 F2 hybrids. a Mean feeding damage for one J. aquatica genotype (JA), one J. vulgaris genotype (JV), and 2 F1 (F1-A and F1 -B) genotypes. Error bars are standard errors, N = 12. J. vulgaris was significantly different from the other genotypes at * P < 0.05. b Distribution frequency for genotypic mean WFT feeding damage of 94 F2 hybrids. N = 3–6 for each genotype. In total, 587 plants were used in WFT bioassay
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3197926&req=5

Fig1: Variation in western flower thrips (WFT) feeding damage (mm2) on Jacobaea aquatica, Jacobaea vulgaris, 2 F1 and 94 F2 hybrids. a Mean feeding damage for one J. aquatica genotype (JA), one J. vulgaris genotype (JV), and 2 F1 (F1-A and F1 -B) genotypes. Error bars are standard errors, N = 12. J. vulgaris was significantly different from the other genotypes at * P < 0.05. b Distribution frequency for genotypic mean WFT feeding damage of 94 F2 hybrids. N = 3–6 for each genotype. In total, 587 plants were used in WFT bioassay
Mentions: Variation in Feeding Damage Feeding damage was genotype-dependent (d.f. = 97,488; F = 5.30; P < 0.001). Plant mass also had effect on feeding damage (d.f. = 1,488; F = 18.44; P < 0.001). Among the two parental genotypes, J. aquatica suffered more feeding damage than J. vulgaris (d.f. = 1, 22; t = 6.18; P < 0.001). Both of the F1 lines were as susceptible as J. aquatica. Among the 94 F2 hybrids, 69 were as susceptible as J. aquatica, 10 were more susceptible than J. aquatica, 15 showed intermediate resistance, 9 were as resistant as J. vulgaris, and none were more resistant than J. vulgaris (Fig. 1, see statistical details in Supplementary Table 1).Fig. 1

Bottom Line: Among 37 individual PAs that were derived from four structural groups (senecionine-, jacobine-, erucifoline- and otosenine-like PAs), the N-oxides of jacobine, jaconine, and jacoline were negatively correlated with feeding damage caused by WFT, and the tertiary amines of jacobine, jaconine, jacoline, and other PAs did not relate to feeding damage.Multiple regression tests suggested that jacobine-like PAs play a greater role in WFT resistance than PAs from other structural groups.We found no evidence for synergistic effects of different PAs on WFT resistance.

View Article: PubMed Central - PubMed

Affiliation: Plant Ecology & Phytochemistry, Institute of Biology, Leiden University, the Netherlands. d.d.cheng@biology.leidenuniv.nl

ABSTRACT
Segregating plant hybrids often have more ecological and molecular variability compared to parental species, and are therefore useful for studying relationships between different traits, and the adaptive significance of trait variation. Hybrid systems have been used to study the relationship between the expression of plant defense compounds and herbivore susceptibility. We conducted a western flower thrips (WFT) bioassay using a hybrid family and investigated the relationship between WFT resistance and pyrrolizidine alkaloid (PA) variation. The hybrid family consisted of two parental (Jacobaea vulgaris and Jacobaea aquatica) genotypes, two F(1) genotypes, and 94 F(2) hybrid lines. The J. aquatica genotype was more susceptible to thrips attack than the J. vulgaris genotype, the two F(1) hybrids were as susceptible as J. aquatica, and susceptibility to WFT differed among F(2) hybrid lines: 69 F(2) lines were equally susceptible compared to J. aquatica, 10 F(2) lines were more susceptible than J. aquatica and 15 F(2) lines were as resistant as J. vulgaris or were intermediate to the two parental genotypes. Among 37 individual PAs that were derived from four structural groups (senecionine-, jacobine-, erucifoline- and otosenine-like PAs), the N-oxides of jacobine, jaconine, and jacoline were negatively correlated with feeding damage caused by WFT, and the tertiary amines of jacobine, jaconine, jacoline, and other PAs did not relate to feeding damage. Total PA concentration was negatively correlated with feeding damage. Among the four PA groups, only the total concentration of the jacobine-like PAs was negatively correlated with feeding damage. Multiple regression tests suggested that jacobine-like PAs play a greater role in WFT resistance than PAs from other structural groups. We found no evidence for synergistic effects of different PAs on WFT resistance. The relationship between PA variation and WFT feeding damage in the Jacobaea hybrids suggests a role for PAs in resistance to generalist insects.

Show MeSH
Related in: MedlinePlus