Limits...
Hippocampal pyramidal cells: the reemergence of cortical lamination.

Slomianka L, Amrein I, Knuesel I, Sørensen JC, Wolfer DP - Brain Struct Funct (2011)

Bottom Line: Distributions of deep and superficial pyramidal cell dendrites and studies in reeler or sparsely GFP-expressing mice indicate that this also applies to afferent pathways.Histological, neurochemical, and connective differences between deep and superficial neurons may correlate with (patho-) physiological phenomena specific to pyramidal cells at different radial locations.We feel that an appreciation of radial subdivisions in the pyramidal cell layer reminiscent of lamination in other cortical areas may be critical in the interpretation of studies of hippocampal anatomy and function.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, University of Zürich, 8057 Zürich, Switzerland. slomianka@anatom.uzh.ch

Show MeSH

Related in: MedlinePlus

Calbindin-immunoreactive (CaBP-ir) and green-fluorescent protein expressing pyramidal cells in a the septal CA1 and b the distal mid-septotemporal CA1 of thy1-GFP (M line) transgenic mice. c CaBP-ir CA1 pyramidal cells in septal CA1 of Wistar rat. Some of the voids left by unstained deep cells are marked with an asterisk. d Zinc-containing pyramidal cells in mid-septotemporal, mid-proximodistal CA1 of Wistar-Kyoto rat. e CaBP-ir CA1 pyramidal cells in temporal one-half of th fox CA1. Lightly CaBP-ir superficial cells are separated from a tier of strongly CaBP-ir, large deep pyramids by virtually unstained cells. f Cells in the septal three-quarters of the elephant shrew CA1 pyramidal cell layer (delimited by open arrows) are completely unstained. The filled arrow marks the boundary between stratum radiatum and stratum lacunosum-moleculare. g CaBP-ir deep CA1 pyramidal cells in the temporal CA1 of the elephant shrew. h CaBP-ir deep CA3 pyramidal cells in naked mole rats, mf: CaBP-ir mossy fibers. Scalebarsa, c and d 20 μm; b, g and h 50 μm; e and f 100 μm
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3197924&req=5

Fig4: Calbindin-immunoreactive (CaBP-ir) and green-fluorescent protein expressing pyramidal cells in a the septal CA1 and b the distal mid-septotemporal CA1 of thy1-GFP (M line) transgenic mice. c CaBP-ir CA1 pyramidal cells in septal CA1 of Wistar rat. Some of the voids left by unstained deep cells are marked with an asterisk. d Zinc-containing pyramidal cells in mid-septotemporal, mid-proximodistal CA1 of Wistar-Kyoto rat. e CaBP-ir CA1 pyramidal cells in temporal one-half of th fox CA1. Lightly CaBP-ir superficial cells are separated from a tier of strongly CaBP-ir, large deep pyramids by virtually unstained cells. f Cells in the septal three-quarters of the elephant shrew CA1 pyramidal cell layer (delimited by open arrows) are completely unstained. The filled arrow marks the boundary between stratum radiatum and stratum lacunosum-moleculare. g CaBP-ir deep CA1 pyramidal cells in the temporal CA1 of the elephant shrew. h CaBP-ir deep CA3 pyramidal cells in naked mole rats, mf: CaBP-ir mossy fibers. Scalebarsa, c and d 20 μm; b, g and h 50 μm; e and f 100 μm

Mentions: Calbindin. The distribution of calbindin immunoreactive (CaBP-ir) pyramidal cells in CA1 of the rat hippocampus probably represents the earliest neurochemical evidence for a radial subdivision of the layer. Throughout most of the transverse extent of the layer, CaBP-ir pyramidal cells are located in the cell-dense superficial part, while deeper pyramidal cells do not contain this protein (Baimbridge and Miller 1982; Rami et al. 1987b; Sloviter 1989; Celio 1990). In the septal hippocampus, CaBP-ir pyramidal cells form a superficial mono- or bilayer above unstained deeper cells (Fig. 4c; also compare with Fig. 2l, m). Only at the proximal limit of CA1, where CaBP-ir becomes weak superficially, CaBP-ir appears in some of the deepest pyramidal cells. The deepest cells are also stained distally, in the vicinity of the subiculum. Baimbridge et al. (1991) have shown that there was virtually no overlap in the labeling of CaBP-negative and CaBP-ir pyramidal cells when animals were injected with 3H-thymidine on E16 and E18, respectively. Notably, calbindin expression is apparently controlled by Zbtb20—being down-regulated in cells in which the expression of Zbtb20 has been blocked and appearing in deep pyramidal cells as the expression of Zbtb20 is induced in these cells (Nielsen et al. 2010). Despite their change in location, pyramidal cells in CA1 of reeler mice retain their identities with regard to calbindin expression, which is strongest in the deep sublayer but weak or absent in superficial cells (Fig. 3d).Fig. 4


Hippocampal pyramidal cells: the reemergence of cortical lamination.

Slomianka L, Amrein I, Knuesel I, Sørensen JC, Wolfer DP - Brain Struct Funct (2011)

Calbindin-immunoreactive (CaBP-ir) and green-fluorescent protein expressing pyramidal cells in a the septal CA1 and b the distal mid-septotemporal CA1 of thy1-GFP (M line) transgenic mice. c CaBP-ir CA1 pyramidal cells in septal CA1 of Wistar rat. Some of the voids left by unstained deep cells are marked with an asterisk. d Zinc-containing pyramidal cells in mid-septotemporal, mid-proximodistal CA1 of Wistar-Kyoto rat. e CaBP-ir CA1 pyramidal cells in temporal one-half of th fox CA1. Lightly CaBP-ir superficial cells are separated from a tier of strongly CaBP-ir, large deep pyramids by virtually unstained cells. f Cells in the septal three-quarters of the elephant shrew CA1 pyramidal cell layer (delimited by open arrows) are completely unstained. The filled arrow marks the boundary between stratum radiatum and stratum lacunosum-moleculare. g CaBP-ir deep CA1 pyramidal cells in the temporal CA1 of the elephant shrew. h CaBP-ir deep CA3 pyramidal cells in naked mole rats, mf: CaBP-ir mossy fibers. Scalebarsa, c and d 20 μm; b, g and h 50 μm; e and f 100 μm
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3197924&req=5

Fig4: Calbindin-immunoreactive (CaBP-ir) and green-fluorescent protein expressing pyramidal cells in a the septal CA1 and b the distal mid-septotemporal CA1 of thy1-GFP (M line) transgenic mice. c CaBP-ir CA1 pyramidal cells in septal CA1 of Wistar rat. Some of the voids left by unstained deep cells are marked with an asterisk. d Zinc-containing pyramidal cells in mid-septotemporal, mid-proximodistal CA1 of Wistar-Kyoto rat. e CaBP-ir CA1 pyramidal cells in temporal one-half of th fox CA1. Lightly CaBP-ir superficial cells are separated from a tier of strongly CaBP-ir, large deep pyramids by virtually unstained cells. f Cells in the septal three-quarters of the elephant shrew CA1 pyramidal cell layer (delimited by open arrows) are completely unstained. The filled arrow marks the boundary between stratum radiatum and stratum lacunosum-moleculare. g CaBP-ir deep CA1 pyramidal cells in the temporal CA1 of the elephant shrew. h CaBP-ir deep CA3 pyramidal cells in naked mole rats, mf: CaBP-ir mossy fibers. Scalebarsa, c and d 20 μm; b, g and h 50 μm; e and f 100 μm
Mentions: Calbindin. The distribution of calbindin immunoreactive (CaBP-ir) pyramidal cells in CA1 of the rat hippocampus probably represents the earliest neurochemical evidence for a radial subdivision of the layer. Throughout most of the transverse extent of the layer, CaBP-ir pyramidal cells are located in the cell-dense superficial part, while deeper pyramidal cells do not contain this protein (Baimbridge and Miller 1982; Rami et al. 1987b; Sloviter 1989; Celio 1990). In the septal hippocampus, CaBP-ir pyramidal cells form a superficial mono- or bilayer above unstained deeper cells (Fig. 4c; also compare with Fig. 2l, m). Only at the proximal limit of CA1, where CaBP-ir becomes weak superficially, CaBP-ir appears in some of the deepest pyramidal cells. The deepest cells are also stained distally, in the vicinity of the subiculum. Baimbridge et al. (1991) have shown that there was virtually no overlap in the labeling of CaBP-negative and CaBP-ir pyramidal cells when animals were injected with 3H-thymidine on E16 and E18, respectively. Notably, calbindin expression is apparently controlled by Zbtb20—being down-regulated in cells in which the expression of Zbtb20 has been blocked and appearing in deep pyramidal cells as the expression of Zbtb20 is induced in these cells (Nielsen et al. 2010). Despite their change in location, pyramidal cells in CA1 of reeler mice retain their identities with regard to calbindin expression, which is strongest in the deep sublayer but weak or absent in superficial cells (Fig. 3d).Fig. 4

Bottom Line: Distributions of deep and superficial pyramidal cell dendrites and studies in reeler or sparsely GFP-expressing mice indicate that this also applies to afferent pathways.Histological, neurochemical, and connective differences between deep and superficial neurons may correlate with (patho-) physiological phenomena specific to pyramidal cells at different radial locations.We feel that an appreciation of radial subdivisions in the pyramidal cell layer reminiscent of lamination in other cortical areas may be critical in the interpretation of studies of hippocampal anatomy and function.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, University of Zürich, 8057 Zürich, Switzerland. slomianka@anatom.uzh.ch

Show MeSH
Related in: MedlinePlus