Limits...
Ameliorating effect of Yokukansan on the development of atopic dermatitis-like lesions and scratching behavior in socially isolated NC/Nga mice.

Funakushi N, Yamaguchi T, Jiang J, Imamura S, Kuhara T, Suto H, Ueki R, Kase Y, Kobayashi H, Ogawa H, Ikeda S - Arch. Dermatol. Res. (2011)

Bottom Line: Yokukansan (YKS) has been used in Japan as a remedy for neurosis, insomnia, and children with night crying.YKS and fexofenadine inhibited the aggravation of skin lesions and decreased TEWL, but only YKS decreased the numbers of scratching and pathologic grooming behaviors.Furthermore, the effects of YKS are suggested to be mediated via glutamate signaling in the skin lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Juntendo University School of Medicine, Hongo, Bunkyo-ku, Tokyo, Japan. nao2794@juntendo.ac.jp

ABSTRACT
Yokukansan (YKS) has been used in Japan as a remedy for neurosis, insomnia, and children with night crying. In a previous study, we reported that YKS controls scratching behavior and inhibits the development of atopic dermatitis (AD)-like lesions in NC/Nga mice. In this study, we investigated the effects of YKS on the development of AD-like lesions in socially isolated NC/Nga mice compared with the effects of fexofenadine and elucidated the mechanism of the ameliorating effect of YKS on the skin lesions. Ten-week-old male NC/Nga mice were divided into three groups (n = 5/group): the conventional control, the YKS-treated, and the fexofenadine-treated groups, and were kept isolated under conventional conditions for 6 weeks. Measurements were made of dermatitis scores and transepidermal water loss (TEWL), scratching and grooming behaviors. Immunohistochemistry and mRNA levels were also evaluated. We performed similar experiments under specific pathogen free (SPF) conditions that served as a SPF control. YKS and fexofenadine inhibited the aggravation of skin lesions and decreased TEWL, but only YKS decreased the numbers of scratching and pathologic grooming behaviors. Immunohistochemistry and RT-PCR revealed that N-methyl-D: -aspartate (NMDA) receptor expression was increased in the skin of conventional control mice and was decreased in YKS-treated mice. Glutamate transporter-1 (GLT-1) mRNA levels were decreased in the skin of conventional control mice and were increased in YKS-treated mice. The results indicate that YKS ameliorates AD-like skin lesions in NC/Nga mice through a mechanism distinct from that of fexofenadine. Furthermore, the effects of YKS are suggested to be mediated via glutamate signaling in the skin lesions.

Show MeSH

Related in: MedlinePlus

Immunohistochemical analysis of NMDA receptor expression in the skin of NC/Nga mice Sections were stained with a fluorescent anti-goat secondary antibody (Alexa Fluor® Anti-Goat IgG) (green). Nuclei were counterstained with 4′-6′-diamidino-2-phenylindole hydrochloride (DAPI) (blue). NMDA receptor expression in the conventional control was increased compared with the SPF control. NMDA receptor expression in the YKS group was decreased compared with the conventional control and the fexofenadine groups. The scale bar represents 100 μm
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3197922&req=5

Fig3: Immunohistochemical analysis of NMDA receptor expression in the skin of NC/Nga mice Sections were stained with a fluorescent anti-goat secondary antibody (Alexa Fluor® Anti-Goat IgG) (green). Nuclei were counterstained with 4′-6′-diamidino-2-phenylindole hydrochloride (DAPI) (blue). NMDA receptor expression in the conventional control was increased compared with the SPF control. NMDA receptor expression in the YKS group was decreased compared with the conventional control and the fexofenadine groups. The scale bar represents 100 μm

Mentions: At the involved-skin lesion, expression of NMDA receptors were increased in the epidermis under the conventional control mice compared with the SPF control mice. After the treatment with YKS, the expression of NMDA receptors was significantly reduced in the conventional control mice, which was similar to that of SPF control mice (Fig. 3).Fig. 3


Ameliorating effect of Yokukansan on the development of atopic dermatitis-like lesions and scratching behavior in socially isolated NC/Nga mice.

Funakushi N, Yamaguchi T, Jiang J, Imamura S, Kuhara T, Suto H, Ueki R, Kase Y, Kobayashi H, Ogawa H, Ikeda S - Arch. Dermatol. Res. (2011)

Immunohistochemical analysis of NMDA receptor expression in the skin of NC/Nga mice Sections were stained with a fluorescent anti-goat secondary antibody (Alexa Fluor® Anti-Goat IgG) (green). Nuclei were counterstained with 4′-6′-diamidino-2-phenylindole hydrochloride (DAPI) (blue). NMDA receptor expression in the conventional control was increased compared with the SPF control. NMDA receptor expression in the YKS group was decreased compared with the conventional control and the fexofenadine groups. The scale bar represents 100 μm
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3197922&req=5

Fig3: Immunohistochemical analysis of NMDA receptor expression in the skin of NC/Nga mice Sections were stained with a fluorescent anti-goat secondary antibody (Alexa Fluor® Anti-Goat IgG) (green). Nuclei were counterstained with 4′-6′-diamidino-2-phenylindole hydrochloride (DAPI) (blue). NMDA receptor expression in the conventional control was increased compared with the SPF control. NMDA receptor expression in the YKS group was decreased compared with the conventional control and the fexofenadine groups. The scale bar represents 100 μm
Mentions: At the involved-skin lesion, expression of NMDA receptors were increased in the epidermis under the conventional control mice compared with the SPF control mice. After the treatment with YKS, the expression of NMDA receptors was significantly reduced in the conventional control mice, which was similar to that of SPF control mice (Fig. 3).Fig. 3

Bottom Line: Yokukansan (YKS) has been used in Japan as a remedy for neurosis, insomnia, and children with night crying.YKS and fexofenadine inhibited the aggravation of skin lesions and decreased TEWL, but only YKS decreased the numbers of scratching and pathologic grooming behaviors.Furthermore, the effects of YKS are suggested to be mediated via glutamate signaling in the skin lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Juntendo University School of Medicine, Hongo, Bunkyo-ku, Tokyo, Japan. nao2794@juntendo.ac.jp

ABSTRACT
Yokukansan (YKS) has been used in Japan as a remedy for neurosis, insomnia, and children with night crying. In a previous study, we reported that YKS controls scratching behavior and inhibits the development of atopic dermatitis (AD)-like lesions in NC/Nga mice. In this study, we investigated the effects of YKS on the development of AD-like lesions in socially isolated NC/Nga mice compared with the effects of fexofenadine and elucidated the mechanism of the ameliorating effect of YKS on the skin lesions. Ten-week-old male NC/Nga mice were divided into three groups (n = 5/group): the conventional control, the YKS-treated, and the fexofenadine-treated groups, and were kept isolated under conventional conditions for 6 weeks. Measurements were made of dermatitis scores and transepidermal water loss (TEWL), scratching and grooming behaviors. Immunohistochemistry and mRNA levels were also evaluated. We performed similar experiments under specific pathogen free (SPF) conditions that served as a SPF control. YKS and fexofenadine inhibited the aggravation of skin lesions and decreased TEWL, but only YKS decreased the numbers of scratching and pathologic grooming behaviors. Immunohistochemistry and RT-PCR revealed that N-methyl-D: -aspartate (NMDA) receptor expression was increased in the skin of conventional control mice and was decreased in YKS-treated mice. Glutamate transporter-1 (GLT-1) mRNA levels were decreased in the skin of conventional control mice and were increased in YKS-treated mice. The results indicate that YKS ameliorates AD-like skin lesions in NC/Nga mice through a mechanism distinct from that of fexofenadine. Furthermore, the effects of YKS are suggested to be mediated via glutamate signaling in the skin lesions.

Show MeSH
Related in: MedlinePlus