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Ameliorating effect of Yokukansan on the development of atopic dermatitis-like lesions and scratching behavior in socially isolated NC/Nga mice.

Funakushi N, Yamaguchi T, Jiang J, Imamura S, Kuhara T, Suto H, Ueki R, Kase Y, Kobayashi H, Ogawa H, Ikeda S - Arch. Dermatol. Res. (2011)

Bottom Line: Yokukansan (YKS) has been used in Japan as a remedy for neurosis, insomnia, and children with night crying.YKS and fexofenadine inhibited the aggravation of skin lesions and decreased TEWL, but only YKS decreased the numbers of scratching and pathologic grooming behaviors.Furthermore, the effects of YKS are suggested to be mediated via glutamate signaling in the skin lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Juntendo University School of Medicine, Hongo, Bunkyo-ku, Tokyo, Japan. nao2794@juntendo.ac.jp

ABSTRACT
Yokukansan (YKS) has been used in Japan as a remedy for neurosis, insomnia, and children with night crying. In a previous study, we reported that YKS controls scratching behavior and inhibits the development of atopic dermatitis (AD)-like lesions in NC/Nga mice. In this study, we investigated the effects of YKS on the development of AD-like lesions in socially isolated NC/Nga mice compared with the effects of fexofenadine and elucidated the mechanism of the ameliorating effect of YKS on the skin lesions. Ten-week-old male NC/Nga mice were divided into three groups (n = 5/group): the conventional control, the YKS-treated, and the fexofenadine-treated groups, and were kept isolated under conventional conditions for 6 weeks. Measurements were made of dermatitis scores and transepidermal water loss (TEWL), scratching and grooming behaviors. Immunohistochemistry and mRNA levels were also evaluated. We performed similar experiments under specific pathogen free (SPF) conditions that served as a SPF control. YKS and fexofenadine inhibited the aggravation of skin lesions and decreased TEWL, but only YKS decreased the numbers of scratching and pathologic grooming behaviors. Immunohistochemistry and RT-PCR revealed that N-methyl-D: -aspartate (NMDA) receptor expression was increased in the skin of conventional control mice and was decreased in YKS-treated mice. Glutamate transporter-1 (GLT-1) mRNA levels were decreased in the skin of conventional control mice and were increased in YKS-treated mice. The results indicate that YKS ameliorates AD-like skin lesions in NC/Nga mice through a mechanism distinct from that of fexofenadine. Furthermore, the effects of YKS are suggested to be mediated via glutamate signaling in the skin lesions.

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Effects of YKS and fexofenadine on the numbers of infiltrating mast cells in the skin of NC/Nga mice The numbers of mast cells in the skin of NC/Nga mice skin inside a 0.25 × 0.25 mm (0.0625 mm2) square were counted using a microscope. The numbers of mast cells of the conventional control group were significantly increased compared with the numbers of mast cells in the SPF control group. The numbers of mast cells of the YKS- and the fexofenadine-treated groups were significantly decreased compared with the numbers of mast cells in the conventional control group. Data are presented as means ± SE (n = 5–6). †p < 0.05, ††p < 0.01 versus SPF control, *p < 0.05, **p < 0.01 versus conventional control, one-way ANOVA followed by Bonferroni/Dunn test
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Fig2: Effects of YKS and fexofenadine on the numbers of infiltrating mast cells in the skin of NC/Nga mice The numbers of mast cells in the skin of NC/Nga mice skin inside a 0.25 × 0.25 mm (0.0625 mm2) square were counted using a microscope. The numbers of mast cells of the conventional control group were significantly increased compared with the numbers of mast cells in the SPF control group. The numbers of mast cells of the YKS- and the fexofenadine-treated groups were significantly decreased compared with the numbers of mast cells in the conventional control group. Data are presented as means ± SE (n = 5–6). †p < 0.05, ††p < 0.01 versus SPF control, *p < 0.05, **p < 0.01 versus conventional control, one-way ANOVA followed by Bonferroni/Dunn test

Mentions: Using histological examination, the numbers of mast cells infiltrating the skin were enumerated. The numbers of infiltrating mast cells in 0.25 × 0.25 mm (0.0625 mm2) squares were counted using a microscope. The numbers of infiltrating mast cells in the YKS-treated or the fexofenadine-treated mice were significantly decreased compared with the conventional control mice (Fig. 2).Fig. 2


Ameliorating effect of Yokukansan on the development of atopic dermatitis-like lesions and scratching behavior in socially isolated NC/Nga mice.

Funakushi N, Yamaguchi T, Jiang J, Imamura S, Kuhara T, Suto H, Ueki R, Kase Y, Kobayashi H, Ogawa H, Ikeda S - Arch. Dermatol. Res. (2011)

Effects of YKS and fexofenadine on the numbers of infiltrating mast cells in the skin of NC/Nga mice The numbers of mast cells in the skin of NC/Nga mice skin inside a 0.25 × 0.25 mm (0.0625 mm2) square were counted using a microscope. The numbers of mast cells of the conventional control group were significantly increased compared with the numbers of mast cells in the SPF control group. The numbers of mast cells of the YKS- and the fexofenadine-treated groups were significantly decreased compared with the numbers of mast cells in the conventional control group. Data are presented as means ± SE (n = 5–6). †p < 0.05, ††p < 0.01 versus SPF control, *p < 0.05, **p < 0.01 versus conventional control, one-way ANOVA followed by Bonferroni/Dunn test
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3197922&req=5

Fig2: Effects of YKS and fexofenadine on the numbers of infiltrating mast cells in the skin of NC/Nga mice The numbers of mast cells in the skin of NC/Nga mice skin inside a 0.25 × 0.25 mm (0.0625 mm2) square were counted using a microscope. The numbers of mast cells of the conventional control group were significantly increased compared with the numbers of mast cells in the SPF control group. The numbers of mast cells of the YKS- and the fexofenadine-treated groups were significantly decreased compared with the numbers of mast cells in the conventional control group. Data are presented as means ± SE (n = 5–6). †p < 0.05, ††p < 0.01 versus SPF control, *p < 0.05, **p < 0.01 versus conventional control, one-way ANOVA followed by Bonferroni/Dunn test
Mentions: Using histological examination, the numbers of mast cells infiltrating the skin were enumerated. The numbers of infiltrating mast cells in 0.25 × 0.25 mm (0.0625 mm2) squares were counted using a microscope. The numbers of infiltrating mast cells in the YKS-treated or the fexofenadine-treated mice were significantly decreased compared with the conventional control mice (Fig. 2).Fig. 2

Bottom Line: Yokukansan (YKS) has been used in Japan as a remedy for neurosis, insomnia, and children with night crying.YKS and fexofenadine inhibited the aggravation of skin lesions and decreased TEWL, but only YKS decreased the numbers of scratching and pathologic grooming behaviors.Furthermore, the effects of YKS are suggested to be mediated via glutamate signaling in the skin lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Juntendo University School of Medicine, Hongo, Bunkyo-ku, Tokyo, Japan. nao2794@juntendo.ac.jp

ABSTRACT
Yokukansan (YKS) has been used in Japan as a remedy for neurosis, insomnia, and children with night crying. In a previous study, we reported that YKS controls scratching behavior and inhibits the development of atopic dermatitis (AD)-like lesions in NC/Nga mice. In this study, we investigated the effects of YKS on the development of AD-like lesions in socially isolated NC/Nga mice compared with the effects of fexofenadine and elucidated the mechanism of the ameliorating effect of YKS on the skin lesions. Ten-week-old male NC/Nga mice were divided into three groups (n = 5/group): the conventional control, the YKS-treated, and the fexofenadine-treated groups, and were kept isolated under conventional conditions for 6 weeks. Measurements were made of dermatitis scores and transepidermal water loss (TEWL), scratching and grooming behaviors. Immunohistochemistry and mRNA levels were also evaluated. We performed similar experiments under specific pathogen free (SPF) conditions that served as a SPF control. YKS and fexofenadine inhibited the aggravation of skin lesions and decreased TEWL, but only YKS decreased the numbers of scratching and pathologic grooming behaviors. Immunohistochemistry and RT-PCR revealed that N-methyl-D: -aspartate (NMDA) receptor expression was increased in the skin of conventional control mice and was decreased in YKS-treated mice. Glutamate transporter-1 (GLT-1) mRNA levels were decreased in the skin of conventional control mice and were increased in YKS-treated mice. The results indicate that YKS ameliorates AD-like skin lesions in NC/Nga mice through a mechanism distinct from that of fexofenadine. Furthermore, the effects of YKS are suggested to be mediated via glutamate signaling in the skin lesions.

Show MeSH
Related in: MedlinePlus