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Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation.

Hsu CK, Liao MH, Tai YT, Liu SH, Ou KL, Fang HW, Lee IJ, Chen RM - Int J Nanomedicine (2011)

Bottom Line: Primary osteoblasts were exposed to 1, 10, 100, and 1000 μg/mL nanoparticles of WEG (nWEG) for 24, 48, and 72 hours did not affect morphologies, viability, or apoptosis of osteoblasts.In addition, nWEG stimulated greater osteoblast maturation than did WEG.Therefore, this study shows that WEG nanoparticles are safer to primary osteoblasts than are normal-sized products, and may promote better bone healing by protecting osteoblasts from apoptotic insults, and by promoting osteogenic maturation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Materials Science and Engineering, National Taipei University of Technology, Taipei, Taiwan.

ABSTRACT
Our previous study showed that Gusuibu (Drynaria fortunei J. Sm.) can stimulate osteoblast maturation. This study was further designed to evaluate the effects of nanoparticles prepared from the water extract of Gusuibu (WEG) on osteoblast survival and maturation. Primary osteoblasts were exposed to 1, 10, 100, and 1000 μg/mL nanoparticles of WEG (nWEG) for 24, 48, and 72 hours did not affect morphologies, viability, or apoptosis of osteoblasts. In comparison, treatment of osteoblasts with 1000 μg/mL WEG for 72 hours decreased cell viability and induced DNA fragmentation and cell apoptosis. nWEG had better antioxidant bioactivity in protecting osteoblasts from oxidative and nitrosative stress-induced apoptosis than WEG. In addition, nWEG stimulated greater osteoblast maturation than did WEG. Therefore, this study shows that WEG nanoparticles are safer to primary osteoblasts than are normal-sized products, and may promote better bone healing by protecting osteoblasts from apoptotic insults, and by promoting osteogenic maturation.

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Effects of the water extract of Gusuibu (WEG) and nanoproducts of the WEG (nWEG) on cell viability. Primary rat osteoblasts isolated from neonatal calvarias were exposed to 1000 μg/mL of WEG (A) and nWEG (B) for 24, 48, and 72 hours. Cell viability was assayed according to a colorimetric method.Notes: Each value represents the mean ± SEM for n = 6. *Indicates that a value significantly differs from the respective control, P < 0.05.
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f3-ijn-6-1405: Effects of the water extract of Gusuibu (WEG) and nanoproducts of the WEG (nWEG) on cell viability. Primary rat osteoblasts isolated from neonatal calvarias were exposed to 1000 μg/mL of WEG (A) and nWEG (B) for 24, 48, and 72 hours. Cell viability was assayed according to a colorimetric method.Notes: Each value represents the mean ± SEM for n = 6. *Indicates that a value significantly differs from the respective control, P < 0.05.

Mentions: Cell viability was analyzed carried out to compare the toxicities of WEG and nWEG to primary rat osteoblasts (Figure 3). Exposure of rat osteoblasts to 1000 μg/mL WEG for 24 and 48 hours did not influence cell viability (Figure 3A). Meanwhile, when the treatment time intervals reached 72 hours, WEG caused a significant 31% decrease in osteoblast viability. In comparison, exposure to 1000 μg/mL nWEG for 24, 48, and 72 hours did not affect the viability of rat osteoblasts (Figure 3B).


Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation.

Hsu CK, Liao MH, Tai YT, Liu SH, Ou KL, Fang HW, Lee IJ, Chen RM - Int J Nanomedicine (2011)

Effects of the water extract of Gusuibu (WEG) and nanoproducts of the WEG (nWEG) on cell viability. Primary rat osteoblasts isolated from neonatal calvarias were exposed to 1000 μg/mL of WEG (A) and nWEG (B) for 24, 48, and 72 hours. Cell viability was assayed according to a colorimetric method.Notes: Each value represents the mean ± SEM for n = 6. *Indicates that a value significantly differs from the respective control, P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3141868&req=5

f3-ijn-6-1405: Effects of the water extract of Gusuibu (WEG) and nanoproducts of the WEG (nWEG) on cell viability. Primary rat osteoblasts isolated from neonatal calvarias were exposed to 1000 μg/mL of WEG (A) and nWEG (B) for 24, 48, and 72 hours. Cell viability was assayed according to a colorimetric method.Notes: Each value represents the mean ± SEM for n = 6. *Indicates that a value significantly differs from the respective control, P < 0.05.
Mentions: Cell viability was analyzed carried out to compare the toxicities of WEG and nWEG to primary rat osteoblasts (Figure 3). Exposure of rat osteoblasts to 1000 μg/mL WEG for 24 and 48 hours did not influence cell viability (Figure 3A). Meanwhile, when the treatment time intervals reached 72 hours, WEG caused a significant 31% decrease in osteoblast viability. In comparison, exposure to 1000 μg/mL nWEG for 24, 48, and 72 hours did not affect the viability of rat osteoblasts (Figure 3B).

Bottom Line: Primary osteoblasts were exposed to 1, 10, 100, and 1000 μg/mL nanoparticles of WEG (nWEG) for 24, 48, and 72 hours did not affect morphologies, viability, or apoptosis of osteoblasts.In addition, nWEG stimulated greater osteoblast maturation than did WEG.Therefore, this study shows that WEG nanoparticles are safer to primary osteoblasts than are normal-sized products, and may promote better bone healing by protecting osteoblasts from apoptotic insults, and by promoting osteogenic maturation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Materials Science and Engineering, National Taipei University of Technology, Taipei, Taiwan.

ABSTRACT
Our previous study showed that Gusuibu (Drynaria fortunei J. Sm.) can stimulate osteoblast maturation. This study was further designed to evaluate the effects of nanoparticles prepared from the water extract of Gusuibu (WEG) on osteoblast survival and maturation. Primary osteoblasts were exposed to 1, 10, 100, and 1000 μg/mL nanoparticles of WEG (nWEG) for 24, 48, and 72 hours did not affect morphologies, viability, or apoptosis of osteoblasts. In comparison, treatment of osteoblasts with 1000 μg/mL WEG for 72 hours decreased cell viability and induced DNA fragmentation and cell apoptosis. nWEG had better antioxidant bioactivity in protecting osteoblasts from oxidative and nitrosative stress-induced apoptosis than WEG. In addition, nWEG stimulated greater osteoblast maturation than did WEG. Therefore, this study shows that WEG nanoparticles are safer to primary osteoblasts than are normal-sized products, and may promote better bone healing by protecting osteoblasts from apoptotic insults, and by promoting osteogenic maturation.

Show MeSH
Related in: MedlinePlus