Limits...
Isolation and characterization of a novel alphanodavirus.

Bai H, Wang Y, Li X, Mao H, Li Y, Han S, Shi Z, Chen X - Virol. J. (2011)

Bottom Line: The host susceptibility test demonstrated that HzNV was able to infect various cell lines ranging from insect cells to mammalian cells.However, only Hz-AM1 appeared to be fully permissive for HzNV, as the mature viral coat protein essential for HzNV particle formation was limited to Hz-AM1 cells.A novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in Hz-AM1 cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Lab of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

ABSTRACT

Background: Nodaviridae is a family of non-enveloped isometric viruses with bipartite positive-sense RNA genomes. The Nodaviridae family consists of two genera: alpha- and beta-nodavirus. Alphanodaviruses usually infect insect cells. Some commercially available insect cell lines have been latently infected by Alphanodaviruses.

Results: A non-enveloped small virus of approximately 30 nm in diameter was discovered co-existing with a recombinant Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) in Hz-AM1 cells. Genome sequencing and phylogenetic assays indicate that this novel virus belongs to the genus of alphanodavirus in the family Nodaviridae and was designated HzNV. HzNV possesses a RNA genome that contains two segments. RNA1 is 3038 nt long and encodes a 110 kDa viral protein termed protein A. The 1404 nt long RNA2 encodes a 44 kDa protein, which exhibits a high homology with coat protein precursors of other alphanodaviruses. HzNV virions were located in the cytoplasm, in association with cytoplasmic membrane structures. The host susceptibility test demonstrated that HzNV was able to infect various cell lines ranging from insect cells to mammalian cells. However, only Hz-AM1 appeared to be fully permissive for HzNV, as the mature viral coat protein essential for HzNV particle formation was limited to Hz-AM1 cells.

Conclusion: A novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in Hz-AM1 cells.

Show MeSH

Related in: MedlinePlus

Phylogenetic tree of HzNV based on the sequence of the coat protein precursor. The predicted amino acids of the coat protein precursor were used to generate a phylogenetic tree by MEGA 4 software with the neighbor-joining method. The bootstrap equals 1,000 replications. The relevant GenBank accession numbers are indicated in the graph. FHV: flock house virus; BBV: Black beetle virus; BoV: Boolarra virus; NoV: Nodamura virus; PaV: Pariacoto virus; TNCL: Tn-5 drived nodavirus; SJNNV: Striped jack nervous necrosis virus; RGNNV: Red-spotted Grouper Nervous Necrosis Virus; ETNNV: Epinephelus tauvina nervous necrosis virus; SGNNV: sevenband grouper nervous necrosis virus; MrNV: Macrobrachium rosenbergii nodavirus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3141682&req=5

Figure 3: Phylogenetic tree of HzNV based on the sequence of the coat protein precursor. The predicted amino acids of the coat protein precursor were used to generate a phylogenetic tree by MEGA 4 software with the neighbor-joining method. The bootstrap equals 1,000 replications. The relevant GenBank accession numbers are indicated in the graph. FHV: flock house virus; BBV: Black beetle virus; BoV: Boolarra virus; NoV: Nodamura virus; PaV: Pariacoto virus; TNCL: Tn-5 drived nodavirus; SJNNV: Striped jack nervous necrosis virus; RGNNV: Red-spotted Grouper Nervous Necrosis Virus; ETNNV: Epinephelus tauvina nervous necrosis virus; SGNNV: sevenband grouper nervous necrosis virus; MrNV: Macrobrachium rosenbergii nodavirus.

Mentions: To determine the relationship between HzNV and other nodaviruses, with respect to evolutionary distance, a phylogenetic tree was generated by comparing protein α of HzNV with other nodaviruses. The results indicate that HzNV is more closely related to alphanodavirus than to betanodavirus (Figure 3). Within the alphanodavirus clade, HzNV is the closest to the black beetle virus in the alphanodavirus genera.


Isolation and characterization of a novel alphanodavirus.

Bai H, Wang Y, Li X, Mao H, Li Y, Han S, Shi Z, Chen X - Virol. J. (2011)

Phylogenetic tree of HzNV based on the sequence of the coat protein precursor. The predicted amino acids of the coat protein precursor were used to generate a phylogenetic tree by MEGA 4 software with the neighbor-joining method. The bootstrap equals 1,000 replications. The relevant GenBank accession numbers are indicated in the graph. FHV: flock house virus; BBV: Black beetle virus; BoV: Boolarra virus; NoV: Nodamura virus; PaV: Pariacoto virus; TNCL: Tn-5 drived nodavirus; SJNNV: Striped jack nervous necrosis virus; RGNNV: Red-spotted Grouper Nervous Necrosis Virus; ETNNV: Epinephelus tauvina nervous necrosis virus; SGNNV: sevenband grouper nervous necrosis virus; MrNV: Macrobrachium rosenbergii nodavirus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3141682&req=5

Figure 3: Phylogenetic tree of HzNV based on the sequence of the coat protein precursor. The predicted amino acids of the coat protein precursor were used to generate a phylogenetic tree by MEGA 4 software with the neighbor-joining method. The bootstrap equals 1,000 replications. The relevant GenBank accession numbers are indicated in the graph. FHV: flock house virus; BBV: Black beetle virus; BoV: Boolarra virus; NoV: Nodamura virus; PaV: Pariacoto virus; TNCL: Tn-5 drived nodavirus; SJNNV: Striped jack nervous necrosis virus; RGNNV: Red-spotted Grouper Nervous Necrosis Virus; ETNNV: Epinephelus tauvina nervous necrosis virus; SGNNV: sevenband grouper nervous necrosis virus; MrNV: Macrobrachium rosenbergii nodavirus.
Mentions: To determine the relationship between HzNV and other nodaviruses, with respect to evolutionary distance, a phylogenetic tree was generated by comparing protein α of HzNV with other nodaviruses. The results indicate that HzNV is more closely related to alphanodavirus than to betanodavirus (Figure 3). Within the alphanodavirus clade, HzNV is the closest to the black beetle virus in the alphanodavirus genera.

Bottom Line: The host susceptibility test demonstrated that HzNV was able to infect various cell lines ranging from insect cells to mammalian cells.However, only Hz-AM1 appeared to be fully permissive for HzNV, as the mature viral coat protein essential for HzNV particle formation was limited to Hz-AM1 cells.A novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in Hz-AM1 cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Lab of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

ABSTRACT

Background: Nodaviridae is a family of non-enveloped isometric viruses with bipartite positive-sense RNA genomes. The Nodaviridae family consists of two genera: alpha- and beta-nodavirus. Alphanodaviruses usually infect insect cells. Some commercially available insect cell lines have been latently infected by Alphanodaviruses.

Results: A non-enveloped small virus of approximately 30 nm in diameter was discovered co-existing with a recombinant Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) in Hz-AM1 cells. Genome sequencing and phylogenetic assays indicate that this novel virus belongs to the genus of alphanodavirus in the family Nodaviridae and was designated HzNV. HzNV possesses a RNA genome that contains two segments. RNA1 is 3038 nt long and encodes a 110 kDa viral protein termed protein A. The 1404 nt long RNA2 encodes a 44 kDa protein, which exhibits a high homology with coat protein precursors of other alphanodaviruses. HzNV virions were located in the cytoplasm, in association with cytoplasmic membrane structures. The host susceptibility test demonstrated that HzNV was able to infect various cell lines ranging from insect cells to mammalian cells. However, only Hz-AM1 appeared to be fully permissive for HzNV, as the mature viral coat protein essential for HzNV particle formation was limited to Hz-AM1 cells.

Conclusion: A novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in Hz-AM1 cells.

Show MeSH
Related in: MedlinePlus